Extraction, Isolation and Identification of a Luteolytic Substance from Bovine Endometrium1

Hansel, William; Shemesh, Mordechai; Hixon, J. E.; Lukaszewska, J

doi:10.1095/biolreprod13.1.30

Cited by 32 publications

(11 citation statements)

References 14 publications

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“…It has been demonstrated that exogenous AA can increase PGF2α production in bovine endometrial epithelial and stromal cells [5,7]. Moreover, the bovine endometrium contains relatively large amounts of AA [20]. The passages and freezing process may cause the epithelial cells to lose of the most of the AA that is stored in the tissue.…”

Section: Discussionmentioning

confidence: 99%

A Passage and Storage System for Isolated Bovine Endometrial Epithelial and Stromal Cells

Murakami

Shibaya

Takeuchi

et al. 2003

Journal of Reproduction and Development

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Abstract. To establish a storage system for isolated endometrial cells, we investigated the basal, oxytocin (OT)-and tumor necrosis factor (TNF) α-stimulated production of prostaglandin (PG) F2α in bovine-passaged and frozen-thawed endometrial cells. Stromal and epithelial cells obtained from cows in the early stage of the estrous cycle (Days 2-5) were frozen at -80 C or further cultured and/or passaged until passage 4 in DMEM/Ham's F-12 supplemented with 10% calf serum. A freshunfrozen primary culture and one-time passaged fresh-unfrozen cells were used as the control. When both unfrozen and frozen cells reached confluence, the culture medium was replaced with fresh medium with 0.1% BSA and the cells were stimulated with OT (100 ng/ml) or TNFα (1 ng/ml) for 4 h.

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Section: Discussionmentioning

confidence: 99%

A Passage and Storage System for Isolated Bovine Endometrial Epithelial and Stromal Cells

Murakami

Shibaya

Takeuchi

et al. 2003

Journal of Reproduction and Development

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“…In another study, showed elevated concentrations of PGF-2a in endometrial tissue and utero-ovarian plasma but no corresponding change in ovarian arterial plasma of cows around the time of expected luteolysis. Hansel et al (1975) concluded from these data that PGF-2a may not be the uterine luteolysin in the cow.…”

Section: Introductionmentioning

confidence: 85%

“…In addition, physiological infusions of PGF-2ct into the uterine vein of cows during the mid-luteal phase of the cycle result in a rapid and precipitous decline in plasma progesterone concentrations (Goding et al, 1971-72). In an attempt to obtain further evidence that PGF-2a is the luteolytic factor in the cow Hansel, Shemesh, Hixon & Lukaszewska (1975) searched for luteolytic activity in endometrium samples of cows around oestrus but found little activity in the fraction containing PGF-2a. Arachidonic acid, on the other hand, was active in the bioassay used to locate the luteolysin and it was present in high concentrations in endometrial tissue.…”

Section: Introductionmentioning

confidence: 99%

Prolongation of the oestrous cycle in cows and ewes after passive immunization with PGF antibodies

Fairclough¹,

Smith²,

McGowan³

1981

Reproduction

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Three cows and 2 sheep were passively immunized against prostaglandin (PG) F on Day 16 and Days 13-15 of the oestrous cycle respectively. The PGF antiplasma was raised in ovariectomized ewes against a PGF-2 alpha-bovine serum albumin complex and showed 100%, 12.5%, 0.3%, less than 0.05% and less than 0.01% cross-reactivity with PGF-2 alpha, PGE-2, PGA-2, PGB-2 and arachidonic acid, respectively. Control animals were given an equivalent amount of ovariectomized ewe plasma. In all passively immunized animals there was evidence of a persistent corpus luteum as indicated by plasma progesterone concentration and the failure of the animals to return to oestrus until at least 29 days after treatment. These data are consistent with previous proposals that PGF-2 alpha is the uterine luteolytic factor in sheep and cattle.

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“…Early studies aimed at identification of the bovine endometrial luteolytic factor(s) resulted in the isolation and identification of arachidonic acid as a luteolytic substance capable of regressing the corpora lutea and decreasing plasma progesterone concentrations in pseudopregnant hysterectomized hamsters (Hansel et al, 1975). Soon thereafter it was demonstrated in in-vivo studies that arachidonic acid injected directly into the bovine corpus luteum is rapidly converted to PGF-2a, resulting in immediate increases in ovarian venous PGF-2a and jugular oestrogen concentrations, followed by declines in jugular plasma progesterone (Shemesh & Hansel, 1975a).…”

Section: Role Of Arachidonic Acid In Bovine Luteal Cellsmentioning

confidence: 99%

Differential origin and control mechanisms in small and large bovine luteal cells

Hansel¹,

Hw²,

Jp³

et al. 2019

Proc

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Extraction, Isolation and Identification of a Luteolytic Substance from Bovine Endometrium1

Cited by 32 publications

References 14 publications

A Passage and Storage System for Isolated Bovine Endometrial Epithelial and Stromal Cells

A Passage and Storage System for Isolated Bovine Endometrial Epithelial and Stromal Cells

Prolongation of the oestrous cycle in cows and ewes after passive immunization with PGF antibodies

Differential origin and control mechanisms in small and large bovine luteal cells

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