Extraction, Identification and Antioxidative Properties of the Flavonoid-Rich Fractions from Leaves and Flowers of Cassia <i>angustifolia</i>

Abstract

Flavonoids identification, total flavonoids content and antioxidant activity of the extracts from the leaves as well as flowers of Cassia angustifolia were determined. Five different extraction techniques were used for the extraction of flavonoids from leaves and flowers. Total flavonoid contents (TFC) in the extracts were determined by UV-visible spectrophotometry. Individual flavonoids were identified and quantified by high-performance liquid chromatography coupled with photodiode array detection and electrospray ionization tandem mass spectrometry (HPLC-PDA-ESI-MS). It has been examined that aqueous ethanol (70%) fractions of C. angustifolia flowers and leaves are highly rich in flavonoids and microwave extraction is the best method for the extraction of individual flavanoid constituents (1 -9) as well as total flavonoid contents. It was also found that compound 8 was absent in the leaves and compounds 2 and 5 could not be identified. The extracts of C. angustifolia flowers and leaves show strong antioxidant activity.

In vitroantioxidant and antimicrobial activities of flavonoids fromPanax notoginsengflowers

“…The amount of total saponins in the PNF is higher than in any other parts of P. notoginseng; in addition, PNF also contain rich bioactive constituents such as flavonoids and polysaccharide (Yang et al 1983;Wang et al 2009;Zhang et al 2009). Flavonoids exhibit significant biological activities, such as antioxidant, anti-cancer, antimicrobial and so on (Middleton 1998;Cushnie & Lamb 2005;Laghari et al 2011;Wu et al 2012). Up to now, only few reports on flavonoids from PNF have been recorded: two flavanoids from buds of P. notoginseng were isolated and identified (Huang et al 2012).…”

Anxiolytic-like effect of Pistacia atlantica fruit in intact and gonadectomized rats subjected to chronic stress

“…Then, 400 gr of bane was washed with water, dried away from direct sunlight, and powdered using an electric mill. The resulting powder was dissolved in hydroalcoholic solvent (70 ethanol/30 water) for 48 hours and soaked and stirred using a magnetic stirrer, after which the resulting material was filtered and ethanol was evaporated in a rotary vacuum evaporator (15). Male rats (250-300 g) were obtained from the Animal Center of Shahid Bahonar University of Kerman and were adapted to the laboratory conditions for 2 weeks before the experiments.…”

Regulation Mechanism of Chlorogenic Acid Accumulation during the Floral Organ Development of Lonicera confusa

“…A standard curve was constructed using 10-50 µg mL Total flavonoid contents (TFC) was determined according to the methods of Laghari et al (2011) with minor modification: Diluted supernatant (1 mL) was mixed with 0.3 mL of sodium nitrite (5%, w/v) and 1 mL of 70% ethanol in a 10 mL volumetric flask and standing for 6 min. And then, 0.3 mL of aluminum chloride (10% w/v) was added, standing for 6 min again.…”