Extraction, fractionation and assessment of antioxidant activities of active components of Aframomum sceptrum seeds

Okwuchi, Nwankwo Patience

doi:10.5897/ajbr2015.0846

Cited by 4 publications

(2 citation statements)

References 23 publications

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“…Fresh leaves of P. biglobosa were collected from a farm in Mkpor, Nsukka LGA of Enugu State, Nigeria and authenticated by John Nwachukwu, a taxonomist at the University of Nigeria, Nsukka and voucher specimen was submitted at the herbarium of Department of Plant Science and Biotechnology (UNH/147). Air-dried and powdered sample was defatted using n-hexane and then extracted by maceration in methanol for 48 h. The filtrate was dried at 40ºC to obtain the crude methanol extract which was fractionated by vacuum-liquid chromatography as described by Nwankwo [13] using silica gel as stationary phase. The solvent fractions were collected and evaporated to dryness and stored at 2-8 o C. Pilot study showed that ethyl acetate fraction (EAFPB) was more active and was then chosen for intervention study.…”

Section: Plant Samplementioning

confidence: 99%

Attenuation of glycation and biochemical aberrations in fructose‐loaded rats by polyphenol‐rich ethyl acetate fraction of Parkia biglobosa (jacq.) Benth. (Mimosaceae) leaves

et al. 2021

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Background Different parts of the Parkia biglobosa plant are employed in traditional medicine in different African communities. However, information ratifying its use and biochemical influence on health is still scanty in literature. Thus, the present study assessed the influence of the ethyl acetate fraction of Parkia biglobosa leaves (EAFPB) on some biochemical parameters of sub-chronic fructose-loaded rats. Methodology Twenty-five Wistar rats were randomized into five groups (n = 5). The normal control group was maintained on normal diet only while the high fructose solution (HFS) control (placebo), reference and treatment groups received high fructose solution (3 g/kg/d b.w of fructose) for 30 days before treatment. Based on pilot study, two doses (100 and 200 mg/kg/d b.w) of EAFPB were selected and were administered to two groups of test animals while the reference group received 300 mg/kg/d b.w. of metformin for 14 days. Thereafter, blood was collected from fasted animals for biochemical analyses for the examination of level of glycated hemoglobin (HbA1c), liver status (alanine and aspartate aminotransferases (ALT and AST) and alkaline phosphatase (ALP) activities, and bilirubin level), lipid profile (total cholesterol, triglyceride, and low- and high-density lipoproteins levels) and lipid peroxidation (malondialdehyde – MDA level). Results EAFPB was shown to have a good DPPH radical scavenging activity (EC50 = 0.395 mg/ml). Chromatographic analysis of EAFPB revealed 28 known flavonoids (mainly kaempferol (21.31 mg/100 g), quercetin (12.84 mg/100 g), and luteolin (6.75 mg/100 g)), four hydrocinnamic acids derivatives (mainly P-coumaric acid (6.73 mg/100 g)), and 11 phenolic acids derivatives (mainly chlorogenic acid (48.18 mg/100 g) and protocatechuic acid (21.58 mg/100 g)). Relative to normal control, it was observed that fructose overload significantly increased serum activities of ALP, ALT, and AST, and levels of MDA, total cholesterol, low density lipoprotein and triglyceride in placebo. However, EAFPB significantly tapered the elevated serum activities of ALP, ALT, and AST. In addition, relative to placebo, the increased levels of HbA1c, MDA, and lipid health markers were also rebated by EAFPB. Conclusions Ethyl acetate fraction of Parkia biglobosa leaves attenuates biochemical aberrations in fructose-loaded rats, an effect attributable to the rich store of polyphenolic compounds in the fraction. Graphical abstract

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Section: Plant Samplementioning

confidence: 99%

Attenuation of glycation and biochemical aberrations in fructose‐loaded rats by polyphenol‐rich ethyl acetate fraction of Parkia biglobosa (jacq.) Benth. (Mimosaceae) leaves

et al. 2021

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“…It has been widely researched and reported to be rich in phytochemicals and possess several ethnopharmocological properties; thus, it is reported for its efficacy in the traditional herbal management of malaria [14], wound healing, management of postnatal malignancies after delivery [15] and the treatment of several models of diabetes mellitus [16,17]. The efficacy of A. sceptrum extracts has been explored in the preservation of food products like meat and the oxidative deterioration of palm oil [18,19]. Its efficacy in the prevention of cyanide toxicity in Wistar rats has been reported by Atinaya, D.U et al, [20].…”

Section: Problem Statement and Analysis Of The Latest Researchmentioning

confidence: 99%

Possible Mechanism of Aframomum Sceptrum Extracts Mediated Modulation of Renal Function after Monosodium Glutamate Exposure

2019

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The objective of the research was to explain the possible mechanism of an earlier reported role of Aframomum sceptrum extract in the modulation of renal function parameters in monosodium glutamate-induced toxicity. Materials and Methods. Similar experimental methods previously reported by us in Ogbeke et al., (2016) were maintained. Results. Monosodium glutamate administration led to a significant elevation of levels of serum and kidney lipid peroxidation due to decrease in the levels of serum and kidney antioxidant enzyme, super oxide dismutase, catalase, gluthathione peroxidase and gluthathione. There was observed increase in oxidative enzyme, aldehyde oxidase, sulphite oxidase, xanthine oxidase and monoamine oxidase activities in serum and kidney after monosodium glutamate consumption. Aframomum sceptrum treatment significantly regulated all altered indices. Conclusions. The study concluded that the ability of Aframomum sceptrum extract to modulate renal function parameters in monosodium glutamate-induced toxicity is dependent on its efficacy in the induction and mobilization of antioxidant defense armory via the increased synthesis of tissue and serum enzymatic and non-enzymatic antioxidants, as well as improved oxidative enzyme activities that mediates the quenching of rising aldehydes and sulfoxides, N-oxides and aromatic oxides within the kidney.

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Biotoxicity of Colocasia esculenta [Linn.]: Involvement of Colocasia esculenta leaf in preservation of staple from Sitophilus zeamais [Motschulsky] attack

Oriyomi

Fagbohun

Oyedeji

et al. 2022

Industrial Crops and Products

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Extraction, fractionation and assessment of antioxidant activities of active components of Aframomum sceptrum seeds

Cited by 4 publications

References 23 publications

Attenuation of glycation and biochemical aberrations in fructose‐loaded rats by polyphenol‐rich ethyl acetate fraction of Parkia biglobosa (jacq.) Benth. (Mimosaceae) leaves

Attenuation of glycation and biochemical aberrations in fructose‐loaded rats by polyphenol‐rich ethyl acetate fraction of Parkia biglobosa (jacq.) Benth. (Mimosaceae) leaves

Possible Mechanism of Aframomum Sceptrum Extracts Mediated Modulation of Renal Function after Monosodium Glutamate Exposure

Biotoxicity of Colocasia esculenta [Linn.]: Involvement of Colocasia esculenta leaf in preservation of staple from Sitophilus zeamais [Motschulsky] attack

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