Extraction, characterization, and in vivo antitumor activity of a novel polysaccharide fromCoriandrum sativum L.

Abstract

A novel polysaccharide was extracted from Coriandrum sativum L. at a yield of 4.56 ± 0.17% (n = 3). The extraction was optimized using response surface methodology: powder-to-liquid ratio 1:21 g/ml, extraction time 188 min, temperature 81°C, and three replicate extractions. The purified polysaccharide had an average molecular weight of 1.30 × 10 6 Da and was composed of rhamnose, arabinose, galactose, glucose, and galacturonic acid in molar ratios of 1.52: 8.14: 20.85:In vivo tests demonstrated that the polysaccharide suppressed H22 tumor growth in mice and protected the immune organs. Annexin V-FITC/PI, PI, and JC-1 staining showed that the primary mechanism of tumor inhibition was the induction of apoptosis and S-phase arrest with apoptosis achieved via a mitochondrial pathway. Practical applicationsCoriandrum sativum L. is used as a culinary spice but its medicinal value has also been widely recognized. A novel polysaccharide was extracted from this herbaceous plant and its structure and bioactivity were investigated. This high-molecular-weight polysaccharide exhibited antitumor effects against H22 cells in mice and had potential to be developed as an anti-liver cancer medicine and functional food supplement.

Structure Elucidation of a Novel Polysaccharide Isolated from Euonymus fortunei and Establishing Its Antioxidant and Anticancer Properties

“…In this research, multiple experiments were performed to understand the monosaccharide composition and antioxidant and anticancer efects of EFPs. As we know, polysaccharides are among the most versatile natural active substances due to their wide variety of pharmacological activities such as antiaging [20][21][22], liver injury protection [23][24][25], blood glucose lowering [26][27][28][29], antifatigue [30,31], immunoregulatory activity [32][33][34][35], and antitumor activity [36][37][38][39]. Presently, frequently used extraction methods for polysaccharides mainly include hot water extraction [40], alcohol extraction [40], ultrasonic-assisted extraction [41], microwave-assisted extraction [42], and infrared-assisted extraction [43].…”

Fingerprinting Evaluation and Gut Microbiota Regulation of Polysaccharides from Jujube (Ziziphus jujuba Mill.) Fruit

“…Solvent (chloroform:n-butyl alcohol = 5:1, v / v ) was added into an aqueous solution of polysaccharides to remove protein three times. After the shock and centrifugation, the water layer was dried to acquire polysaccharide powders by freeze-drying [ 38 ] (Chang et al, 2022).…”

Research Progress of Polysaccharide-Gold Nanocomplexes in Drug Delivery

“…For example, Dendrobium polysaccharides, Astragalus polysaccharides, Lycium barbarum polysaccharides, and panax notoginseng polysaccharides have shown this effect [ 44 , 45 , 46 , 47 ]. Polysaccharides from food sources, such as coriander polysaccharides, pomegranate polysaccharides, chestnut polysaccharides, and papaya polysaccharides, also demonstrate antitumor effects [ 48 , 49 , 50 , 51 ].…”

“…For example, Dendrobium polysaccharides, Astragalus polysaccharides, Lycium barbarum polysaccharides, and panax notoginseng polysaccharides have shown this effect [44][45][46][47]. Polysaccharides from food sources, such as coriander polysaccharides, pomegranate polysaccharides, chestnut polysaccharides, and papaya polysaccharides, also demonstrate antitumor effects [48][49][50][51]. The relative molecular mass distribution of polysaccharides, the composition of sugar groups, the type of glycosidic bonding and the way adjacent glycosidic bonds are connected, the presence or absence of branched chains as well as their position and length, and the non-sugar modifications may have a significant effect on their activity, as shown in Figure 2.…”