Extraction and Separation of Phycocyanin from &lt;i&gt;Spirulina&lt;/i&gt; using Aqueous Two-Phase Systems of Ionic Liquid and Salt

Zhang, Xifeng; Zhang, Fenqin; Luo, Guanghong; Shu, Yang; Wang, Danxia

doi:10.12691/jfnr-3-1-3

Cited by 23 publications

(17 citation statements)

References 23 publications

(29 reference statements)

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“…Under this condition, the high temperature led to the destruction in hydrogen bonding interaction between the surface water of protein and amino acid residue and thus reduced the purity ratio (Zhang et al . ).…”

Section: Resultsmentioning

confidence: 97%

The Optimized Concentration and Purity of Spirulina platensis C-Phycocyanin: A Comparative Study on Microwave-Assisted and Ultrasound-Assisted Extraction Methods

Aftari

Rezaei

Mortazavi

et al. 2015

Journal of Food Processing and Preservation

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Microwave-assisted (MAE) and ultrasound-assisted (UAE) extraction methods to efficiently extract the C-phycocyanin (C-PC) from the blue-green algae, Spirulina platensis, were compared. The results showed that the linear and interaction effects of two parameters of extraction time and buffer pH on C-PC concentration and purity ratio were significant. The C-PC concentration was enhanced by increasing extraction time up to 7 and 25 min for UAE (2.84 mg/mL) and MAE (4.54 mg/ mL) processes, respectively. The extraction time significantly had more effect than the buffer pH value on the purity ratio. The highest purity ratio of C-PC in UAE (0.65) and MAE (1.27) technologies was obtained in buffer pH values of 6.0 and 7.0, respectively. The C-PC extracted from the MAE system significantly had higher concentration and purity than that obtained using the UAE procedure. Thus, MAE as an efficient method can be applied to extract heat-sensitive C-PC from S. platensis in an industrial scale.

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Section: Resultsmentioning

confidence: 97%

The Optimized Concentration and Purity of Spirulina platensis C-Phycocyanin: A Comparative Study on Microwave-Assisted and Ultrasound-Assisted Extraction Methods

Aftari

Rezaei

Mortazavi

et al. 2015

Journal of Food Processing and Preservation

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“…They found that 82.7–100% of the protein, bovine serum albumin (BSA), migrated to the top phase, while the carbohydrates were preferentially concentrated in the bottom phase. These results demonstrated that IL-ABS were effective in the selective separation of proteins and carbohydrates, and in this sense, further studies were carried out. − Nonetheless, to the best of our knowledge, the fractionation of polysaccharides and proteins and the isolation of specific polysaccharide structures have never been reported in a single step and using IL-based ABS.…”

Section: Introductionmentioning

confidence: 86%

Fractionation of Isochrysis galbana Proteins, Arabinans, and Glucans Using Ionic-Liquid-Based Aqueous Biphasic Systems

Santos

Trigo

Maricato

et al. 2018

ACS Sustainable Chem. Eng.

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Isochrysis galbana (I. galbana) is a microalgae species rich in biomolecules of high commercial value and industrial relevance. Despite the numerous studies done on the extraction of different compounds from microalgae, the complete valorization of the biomass under the biorefinery concept is scarce or even inexistent. The mixture of different compounds found in the different extracts obtained from the microalgae biomass is one of the most important drawbacks in the field. Despite the large interest of academia and industry in the different classes of bioactive compounds composing microalgae, these are still poorly explored due to the low efficiency of the downstream processes used up to date. In this context, this work proposes the development of an efficient purification process by applying ionic liquid (IL)-based aqueous biphasic systems (ABS) to separate proteins from arabinose- and glucose-rich polysaccharides. For this purpose, the nature of the inorganic salt, the IL structural features (anion and alkyl chain length) and the extraction point (using different inorganic salt concentrations) were the conditions optimized. After the proper selection of the most performant IL-ABS (%EEcarb = 71.21 ± 5.21% to the bottom phase and %EEprot = 100% to the top phase), a complete downstream process was developed and implemented, in which the isolation of the target biomolecules (proteins, arabinose- and glucose-rich polysaccharides) and the reuse of the phase formers and main solvents applied was considered. In summary, the approach proposed using IL-ABS appears as a simple and efficient method of purification easily integrated into continuous flow processes, thus demonstrating its industrial potential.

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“…The ATPS system, formed by [Emim][EtSO 4 ] (IL), K 2 HPO 4 (salt), and water, was prepared in 25 mL centrifuge glass tubes that contained the appropriate amounts of all the three components above. Before adding the crude extract into the ATPS system, the pH was adjusted to 6.5 using HCl (ACS reagent, 37%, Sigma-Aldrich) because PC is stable within the pH range 6.0–7.0 and starts degradation at pH values above 7. , Then, the ATPS system and the crude extract were mixed in a vortex for 1 min. The blend was centrifuged at 4000 rpm for 5 min to complete the separation of both phases.…”

Section: Methodsmentioning

confidence: 99%

Novel Biorefinery Approach for Phycocyanin Extraction and Purification and Biocrude Production from Arthrospira platensis

Sánchez-Laso

Espada

Rodríguez

et al. 2023

Ind. Eng. Chem. Res.

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A new biorefinery from Arthrospira platensis was proposed to obtain phycocyanin (PC) and a biocrude by hydrothermal liquefaction (HTL). PC is a high-added-value phycobiliprotein widely used as a food colorant and in the nutraceutical and pharmaceutical industries. However, the use of conventional solvents in the extraction process and the purity grade of the extract are shortcomings in bioproduct production. PC was extracted using a reusable ionic liquid [EMIM][EtSO4], achieving a PC purity of the lowest commercial grade. Therefore, two downstream processes were applied: (1) dialysis + precipitation and (2) aqueous two-phase system (ATPS) + dialysis + precipitation. After the second purification process, the PC purity increased remarkably to reach the analytical grade for pharmaceutical and nutraceutical applications. The waste biomass (WB) obtained in the PC extraction was valorized by hydrothermal liquefaction (HTL) to produce a biocrude. The biocrude yield and composition remarkably enhanced using isopropanol at 350 °C as a cosolvent.

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Extraction and Separation of Phycocyanin from <i>Spirulina</i> using Aqueous Two-Phase Systems of Ionic Liquid and Salt

Cited by 23 publications

References 23 publications

The Optimized Concentration and Purity of Spirulina platensis C-Phycocyanin: A Comparative Study on Microwave-Assisted and Ultrasound-Assisted Extraction Methods

The Optimized Concentration and Purity of Spirulina platensis C-Phycocyanin: A Comparative Study on Microwave-Assisted and Ultrasound-Assisted Extraction Methods

Fractionation of Isochrysis galbana Proteins, Arabinans, and Glucans Using Ionic-Liquid-Based Aqueous Biphasic Systems

Novel Biorefinery Approach for Phycocyanin Extraction and Purification and Biocrude Production from Arthrospira platensis

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