Extraction and Quantitation of Phytosterols from Edible Brown Seaweeds: Optimization, Validation, and Application

Chen, Zhen; Shen, Nianqiu; Wu, Xunzhi; Jia, Jiaping; Wu, Yue; Chiba, Hitoshi; Hui, Shu‐Ping

doi:10.3390/foods12020244

Cited by 6 publications

(3 citation statements)

References 45 publications

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“…MestReNova 9.0 (Mestrelab Research, S.L., Santiago de Compostela, Spain) was used to process all the acquired raw data, including phase correction, baseline adjustment, and integration, which were conducted manually, as previously described [ 18 , 23 ]. The average values from six times replicated processing were performed for all the phase correction and integration procedures to minimize analyst error [ 24 ].…”

Section: Methodsmentioning

confidence: 99%

Quantitation of L-cystine in Food Supplements and Additives Using 1H qNMR: Method Development and Application

Chen

Lian

Zhou

et al. 2023

Foods

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Cystine-enriched food supplements are increasingly popular due to their beneficial health effects. However, the lack of industry standards and market regulations resulted in quality issues with cystine food products, including cases of food adulteration and fraud. This study established a reliable and practical method for determining cystine in food supplements and additives using quantitative NMR (qNMR). With the optimized testing solvent, acquisition time, and relaxation delay, the method exhibited higher sensitivity, precision, and reproducibility than the conventional titrimetric method. Additionally, it was more straightforward and more economical than HPLC and LC-MS. Furthermore, the current qNMR method was applied to investigate different food supplements and additives regarding cystine quantity. As a result, four of eight food supplement samples were found to be inaccurately labeled or even with fake labeling, with the relative actual amount of cystine ranging from 0.3% to 107.2%. In comparison, all three food additive samples exhibited satisfactory quality (the relative actual amount of cystine: 97.0–99.9%). Notably, there was no obvious correlation between the quantifiable properties (price and labeled cystine amount) of the tested food supplement samples and their relative actual amount of cystine. The newly developed qNMR-based approach and the subsequent findings might help standardization and regulation of the cystine supplement market.

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Section: Methodsmentioning

confidence: 99%

Quantitation of L-cystine in Food Supplements and Additives Using 1H qNMR: Method Development and Application

Chen

Lian

Zhou

et al. 2023

Foods

Self Cite

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“…Additionally, the detection of phytosterols assumes a paramount role in scientific and industrial applications. Various methods, such as Gas Chromatography (GC) and Fourier Transform Infrared Spectroscopy (FTIR), are instrumental in detecting and analyzing phytosterols, advancing our understanding of these compounds [3].…”

Section: Figure 1 Chemical Structure Of Phytosterolmentioning

confidence: 99%

Advances in the source, preparation, and application of phytosterols

Xinyue

2024

TNS

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Phytosterols, also referred to as plant sterols, represent naturally occurring compounds exclusive to the plant kingdom. This comprehensive review delves into the multifaceted realm of phytosterols, encompassing their structural characteristics and physiological functions. A keen focus is placed on elucidating their sources, with an emphasis on their abundance in unprocessed materials. The paper meticulously explores the diverse methods employed in the preparation of phytosterols, concurrently scrutinizing the detection techniques applied in their analysis. Within the medical domain, phytosterols have gained recognition for their efficacy in reducing LDL cholesterol levels, thereby proving instrumental in averting cardiovascular diseases, addressing breast cancer, and managing metabolic disorders. In the cosmetic industry, plant-based oils, enriched with polyunsaturated fatty acids, function as potent antioxidants and moisturizers, counteracting the detrimental effects of free radicals. Furthermore, in the realm of the food industry, phytosterols play a pivotal role in the construction of Nanostructured Lipid Carriers (NLCs), preserving the bioavailability of various compounds. This review article stands out for its synthesis of existing research, offering succinct summaries of each section while adeptly bridging identified research gaps. Its intrinsic value lies in its potential to serve as a crucial resource for researchers, fostering deeper investigations and streamlining research endeavors in the medical, cosmetic, and food sectors.

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“…Response surface methodology (RSM) through Box-Behnken design (BBD) is a powerful tool to predict the best extraction conditions leading to optimal desired experimental responses. Recent published literatures have demonstrated this method combination for the efficient extraction of seaweed phytosterols [20], plant antioxidants [21] and mushroom peptides [22]. A minimum of three levels of each shortlisted parameter is required for the BBD to fit a second-order regression model (quadratic model) [21].…”

Section: Introductionmentioning

confidence: 99%

Optimization of an Innovative Hydrothermal Processing on Prebiotic Properties of Eucheuma denticulatum, a Tropical Red Seaweed

2023

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Seaweed is a sustainable source of marine oligosaccharides that potentially could be used as a prebiotic ingredient for functional food development. The study aims to optimize the oligosaccharide preparation through thermal hydrolysis of an under-utilized red seaweed, Eucheuma denticulatum. Response surface methodology (RSM) applying Box–Behnken design (BBD) was used on three parameters including temperature (105–135 °C), hydrolysis time (15–35 min) and sulfuric acid concentration (0.05–0.2 M). Optimized fractions with good prebiotic activity were characterized using high-performance size-exclusion chromatography (HP-SEC) and Fourier transform infrared spectroscopy (FT-IR). Eucheuma denticulatum oligosaccharides fraction 1 (ED-F1) was shown to promote the growth of beneficial gut microbiota including Lactobacillus plantarum, L. casei, L. acidophilus, Bifidobacterium animalis and B. longum with the highest prebiotic activity score of 1.64 ± 0.17. The optimization studies showed that hydrolysis time was the most significant parameter for the oligosaccharides yield. Optimal hydrolysis conditions for ED-F1 were 120 °C, 21 min, 0.12 M H2SO4 with the highest yield achieved (11.15 g/100 g of dry weight). The molecular weight of ED-F1 was determined at 1025 Da while FT-IR analysis revealed the presence of sulfated oligosaccharides with similar characteristics of i-carrageenan. These findings signify the innovative method for the efficient production of seaweed derived prebiotic oligosaccharides, which could be a promising source of functional food ingredients for the development of health foods and beverages.

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Extraction and Quantitation of Phytosterols from Edible Brown Seaweeds: Optimization, Validation, and Application

Cited by 6 publications

References 45 publications

Quantitation of L-cystine in Food Supplements and Additives Using 1H qNMR: Method Development and Application

Quantitation of L-cystine in Food Supplements and Additives Using 1H qNMR: Method Development and Application

Advances in the source, preparation, and application of phytosterols

Optimization of an Innovative Hydrothermal Processing on Prebiotic Properties of Eucheuma denticulatum, a Tropical Red Seaweed

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