Extraction and quantification of melatonin in cornelian cherry (Cornus mas L.) by ultra-fast liquid chromatography coupled to fluorescence detector (UFLC-FD)

Abstract

Wild edible plants (WEPs) can be widely found in the world and defined as native species that grow naturally in their natural habitat. They have become part of the traditional food as human diet and used in folk medicine to treat diseases. They are very rich in terms of nutraceuticals. Melatonin is a natural hormone providing several benefits for human health. It has functions such as regulating growth and development and increasing tolerance to environmental stress factors in plants. It is stated that the serum melatonin level in humans increases after intake of foods containing melatonin. This study examined the presence of melatonin in wild grown cornelian cherry fruits by UFLC-FD and determined suitable extraction and chromatographic conditions. The optimum mobile phase, excitation/emission wavelength, and extraction solvent were determined as methanol: water: acetic acid, 275/345 nm, and methanol: water: HCl, respectively. Melatonin content in fruits ranged from 130.82 to 201.84 ng g−1 in fresh fruit.

Changes in the Phenolic, Melatonin, Sugar Contents and Antioxidant Capacity, Depending on Ripening Stage in Different Cornelian Cherry (Cornus mas L.) Fruits

“…It is a natural hormone providing several benefits for human health, a potent antioxidant scavenging free radical species and stimulating antioxidant enzymes. In plants, it has functions such as flowering, photoperiodicity, growth, development, and increasing tolerance to environmental stress factors [3,19,20] . In present study, the change in melatonin content was examined at three stages of CC fruit growth.…”

“…In plants, it has functions such as flowering, photoperiodicity, growth, development, and increasing tolerance to environmental stress factors. [3,19,20] In present study, the change in melatonin content was examined at three stages of CC fruit growth. At the beginning of fruit growth (unripe stage), melatonin content was pretty high.…”

“…The solvent mixture of methanol: water: acetic acid (55 : 44.9:0.1 v/v/v) was used as the mobile phase. [3] Limit of detection (LOD) and limit of quantification (LOQ) was calculated by multiplying 3.3 and 10 by the standard deviation of the blank solution(s), respectively. For recovery, the sample was spiked with a known amount of phenolic compound standard, and then both the spiked and unspiked samples were measured to determine the amount of phenolic compounds and melatonin by UFLC.…”

“…These plants have recently received more attention due to their health benefits and have been the subject of many studies. [1][2][3] The cornelian cherry (CC) is a wild edible plant that is considered one of those medicinal plants with important nutritional and therapeutic properties.…”

Relationship Between Phenolic Content Determined by LC/MS/MS and Antioxidant Capacity and Enzyme Inhibition of Cyclotrichium niveum L.

“…where X is the mean concentration of the blank and SD is standard deviation of the blank. [24] DPPH * Free Radical Scavenging Activity DPPH * free radical scavenging activity of C. niveum extracts was carried out with the method of Blois. [25] Different volumes of extracts (10, 20, 40 μL) were taken from stock solutions (1 mg/mL) and 1 mL of DPPH * (0.135 mM) solution prepared in ethanol was added to them, and ethanol was added to the solution in the tube until the volume was 4 mL.…”