Extraction and Quantification of Lipids from Plant or Algae

Abstract

In plants and algae, the glycerolipidome changes in response to environmental modifications. For instance, in phosphate starvation, phospholipids are degraded and replaced by nonphosphorus lipids and in nitrogen starvation, storage lipids accumulate. In addition to the well-known applications of oil crops for food, algae lipids are becoming a model for potential applications in health, biofuel, and green chemistry and are used as a platform for genetic engineering. It is therefore important to measure accurately and quickly the glycerolipid content in plants and algae. Here we describe the methods to extract the lipid, quantify the fatty acid amount of the lipid extract and to quantify the different lipid classes that are present in these samples.

The Vps13-like protein BLTP2 is pro-survival and regulates phosphatidylethanolamine levels in the plasma membrane to maintain its fluidity and function

“…Total fatty-acid quantification was performed using gas-chromatography with Flame Ionization Detector (GC-FID) as described in 45 . For LC-MS/MS analyses, the lipid extracts corresponding to 25 nmole of total fatty acids were dissolved in 100 µL of chloroform/methanol [2:1, (v/v)] containing 125 pmole of each internal standard.…”