Extraction and purification of ε-poly-<scp>l</scp>-lysine from fermentation broth using an ethanol/ammonium sulfate aqueous two-phase system combined with ultrafiltration

Chen, Xusheng; Diao, Wenjiao; Ma, Yu; Mao, Zhangming

doi:10.1039/d0ra04245e

Cited by 12 publications

(3 citation statements)

References 29 publications

(30 reference statements)

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“…An aqueous biphasic system can also be combined with ultra-filtration for large scale ε-PL purification. In this system, ε-PL from culture broth was subjected to triplicate extraction by ethanol/ammonium aqueous two-phase system, followed by desalting by ultra-filtration, which together provided new avenues for ε-PL purification ( Chen et al, 2020 ). Although it resulted in a high purity (92.39%) ε-PL product at an acceptable recovery ratio (87.72%), this extraction method may cause some the environmental and economic problems.…”

Section: Downstream Processesmentioning

confidence: 99%

Epsilon-poly-L-lysine: Recent Advances in Biomanufacturing and Applications

Wang

Zhang

et al. 2021

Front. Bioeng. Biotechnol.

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ε-poly-L-lysine (ε-PL) is a naturally occurring poly(amino acid) of varying polymerization degree, which possesses excellent antimicrobial activity and has been widely used in food and pharmaceutical industries. To provide new perspectives from recent advances, this review compares several conventional and advanced strategies for the discovery of wild strains and development of high-producing strains, including isolation and culture-based traditional methods as well as genome mining and directed evolution. We also summarize process engineering approaches for improving production, including optimization of environmental conditions and utilization of industrial waste. Then, efficient downstream purification methods are described, including their drawbacks, followed by the brief introductions of proposed antimicrobial mechanisms of ε-PL and its recent applications. Finally, we discuss persistent challenges and future perspectives for the commercialization of ε-PL.

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Section: Downstream Processesmentioning

confidence: 99%

Epsilon-poly-L-lysine: Recent Advances in Biomanufacturing and Applications

Wang

Zhang

et al. 2021

Front. Bioeng. Biotechnol.

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“…Under the optimal conditions for adsorption and desorption on Amberlite IRC-50 resin, a final ε-PL with a purity of 92.4% was obtained with a 75% recovery [38]. Furthermore, an alcohol/salt aqueous two-phase system (ATPS) was also developed, in which an ATPS, consisting of 20% (w/w) ethanol and 20% (w/w) ammonium sulfate at pH 9.5, was applied to purify ε-PL from fermentation medium with triplicate extractions, and a final ε-PL product with 92.4% purity and 87.7% recovery was obtained with the combination of desalting by ultrafiltration [39].…”

Section: Separation and Purification Of ε-Plmentioning

confidence: 99%

Recent advances in microbial ε-poly-L-lysine fermentation and its diverse applications

Mao

Zhang

et al. 2022

Biotechnol Biofuels

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The naturally occurring homo-polyamide biopolymer, ε-poly-L-lysine (ε-PL) consists of 25–35 L-lysine residues with amide linkages between α-carboxyl groups and ε-amino groups. ɛ-PL exhibits several useful properties because of its unusual structure, such as biodegradability, water solubility, no human toxicity, and broad-spectrum antibacterial activities; it is widely applied in the fields of food, medicine, clinical chemistry and electronics. However, current industrial production of ε-PL is only performed in a few countries. Based on an analysis of the physiological characteristics of ε-PL fermentation, current advances that enhance ε-PL fermentation, from strain improvement to product isolation are systematically reviewed, focusing on: (1) elucidating the metabolic pathway and regulatory mechanism of ε-PL synthesis; (2) enhancing biosynthetic performance through mutagenesis, fermentation optimization and metabolic engineering; and (3) understanding and improving the biological activity and functional properties of ε-PL. Finally, perspectives on engineering and exploiting ε-PL as a source material for the production of various advanced materials are also discussed, providing scientific guidelines for researchers to further improve the ε-PL fermentation process.

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“…Aqueous two-phase systems (ATPS) have been widely used in separations of biomolecules such as proteins, polypeptides, enzymes [1][2][3][4][5][6], bioactive compounds [7,8], and natural dyes [9][10][11][12][13][14][15], among others. Since both phases are mainly made up of water, they provide mild favorable conditions needed to preserve activities of biomolecules [16].…”

Section: Introductionmentioning

confidence: 99%

Anthocyanin Partition in Aqueous Two‐Phase Systems Based on Isopropanol and Sodium/Ammonium Sulfate

et al. 2022

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Partitioning of anthocyanins contained in the crude extract of Syzygium cumini fruit was investigated in aqueous two‐phase systems (ATPS) made up of isopropanol and Na2SO4/(NH4)2SO4. Na2SO4 induced ATPS formation more effectively than (NH4)2SO4. The increase in temperature enhanced phase separation in the (NH4)2SO4 ATPS, while the opposite occurred in the Na2SO4 ATPS. The higher the overall mixture concentration or temperature, the higher the values of tie‐line length and slope. In all systems and conditions, anthocyanins preferentially partitioned to the top phase. The partition coefficient and theoretical recovery yield in the top phase varied in the ranges 1.14–1.77 and 53.31–63.87 % in the (NH4)2SO4 ATPS, and in the ranges 1.94–21.50 and 65.90–95.55 % in the Na2SO4 ATPS.

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Extraction and purification of ε-poly-l-lysine from fermentation broth using an ethanol/ammonium sulfate aqueous two-phase system combined with ultrafiltration

Abstract: Natural antimicrobial agent ε-poly-l-lysine could be alternative prepared by ethanol/ammonium sulfate ATPS combined with ultrafiltration from the microbial feedstock.

Cited by 12 publications

References 29 publications

Epsilon-poly-L-lysine: Recent Advances in Biomanufacturing and Applications

Epsilon-poly-L-lysine: Recent Advances in Biomanufacturing and Applications

Recent advances in microbial ε-poly-L-lysine fermentation and its diverse applications

Anthocyanin Partition in Aqueous Two‐Phase Systems Based on Isopropanol and Sodium/Ammonium Sulfate

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