Extraction and purification of recombinant human serum albumin from Pichia pastoris broths using aqueous two-phase system combined with hydrophobic interaction chromatography

Three-liquid-phase salting-out extraction of effective components from waste liquor of processing sea cucumber

“…1,3-propanediol, 2,3-butanediol, biobutanol, lactic acid and succinic acid Jiang et al, 2009;Sun et al, 2009Sun et al, , 2014Li et al, 2009Li et al, , 2010Li et al, , 2011Dai et al, 2011), natural products, e.g. ␣-galactosidase, oil and alginates and recombinant human albumin (Dhananjay and Mulimani, 2009;Dong et al, 2012). The three-liquid-phase salting-out extraction has been applied in the isolation of complex natural products e.g.…”

Phase separation in a salting-out extraction system of ethanol–ammonium sulfate

“…In order to obtain some Table 4 The phase continuity for three different tie lines with phase volume ratio near the inversion point at high vortex frequency (6). …”

“…The application of salting-out extraction for the bio-based chemicals has been recently reviewed in detail [2]. SOESs have also been used in the recovery of proteins [6,7], enzymes [4,8] and natural products [9][10][11]. However, even though a great amount of reports have been published on the separation of diverse target products with high partition coefficient and recovery on the laboratory scale, this technology has not been used in industrial scale, partially due to the limited engineering knowledge and understanding of this system.…”

“…However, even though a great amount of reports have been published on the separation of diverse target products with high partition coefficient and recovery on the laboratory scale, this technology has not been used in industrial scale, partially due to the limited engineering knowledge and understanding of this system. Almost all of the batch extraction experiments were conducted following an empirical procedure (centrifugation, settling overnight or achieve complete phase equilibrium) without considering the time of the phase separation process [5][6][7][8][10][11][12]. However, these data are necessary for industrial application because the rate of phase separation determines the equipment design and thus the operation costs.…”

An environment‐friendly approach to isolate and purify glucan from spent cells of recombinant Pichia pastoris and the bioactivity characterization of the purified glucan

“…With proper culture medium and growth conditions, P. pastoris can grow to a cell density that is comparable to an OD 600 value of 500, with the yeast cells occupying about 40% of the volume of the culture suspension . Most of the recombinant proteins in P. pastoris are designed to be secreted into the culture medium and harvested by filtration, centrifugation, or aqueous two‐phase extraction . The spent cell bodies consequently become a waste by‐product.…”