Extraction and Purification of R-Phycoerythrin Alpha Subunit from the Marine Red Algae Pyropia Yezoensis and Its Biological Activities

Abstract

Phycoerythrin is a major light-harvesting pigment of red algae and cyanobacteria that is widely used as a fluorescent probe or as a colorant in the food and cosmetic industries. In this study, phycoerythrin was extracted from the red algae Pyropia yezoensis and purified by ammonium sulfate precipitation and various chromatography methods. The purified phycoerythrin was analyzed by UV-visible and fluorescence spectroscopy. The isolated pigment had the typical spectrum of R-phycoerythrin, with a trimmer state with absorbance maxima at 497, 536, and 565 nm. It was further purified and identified by LC-MS/MS and Mascot search. It showed a 100% sequence similarity with the R-phycoerythrin alpha subunit of Pyropia yezoensis. The molecular mass was 17.97 kDa. The antioxidant activity of the purified R-phycoerythrin alpha subunit was analyzed. It showed significant antioxidant activity in ABTS and FRAP assays and had significant cytotoxicity against HepG2 cells.

Phycobiliproteins: Structural aspects, functional characteristics, and biotechnological perspectives

“… [130] PBP Biological activity Source of PBP / Biological Effect Proposed Application Reference PE Antioxidant Pyropia yezoensis / PE had in vitro antioxidant activity and cytotoxicity against HepG2 cells (hepatocellular carcinoma cells) Prevention of the hepatocellular carcinoma cells. [131] Phormidium persicinum / C-PE reduce expression of nephrin and podocin in a mouse model of HgCl 2 -induced acute kidney injury. Reduced activation of IRE1α pathway and avoids caspase-mediated cell death.…”

Antioxidant and chemoprotective peptides from simulated gastrointestinal digested (SGID) protein hydrolysate of Pyropia yezoensis against acetaminophen-induced HepG2 cells

“…The relatively intense bands at 29-33 and 18-22 kDa were likely to correspond to phycobiliproteins (e.g., phycoerythrin), which are typical pigment proteins related to photosynthesis in red seaweed species. Phycobiliprotein from P. yezoensis has previously been reported [23]. Figure 1b shows the glycoproteins present in P. yezoensis.…”

“…Phycobiliprotein from P . yezoensis has previously been reported [ 23 ]. Figure 1 b shows the glycoproteins present in P .…”

“…Dialysis was performed with distilled water using a 2 kDa dialysis membrane to remove the ammonium sulfate from the precipitated pellet. The soluble protein was collected, freeze-dried, and stored at 4 °C [ 23 ].…”

“…Previous study with the recombinant protein isolated from Pyropia yezoensis has also increased the cell viability in the APAP-induced HepG2 cells in a dose-dependent manner [ 48 ]. Additionally, a study by [ 23 ] discovered the prominent cell viability in APAP-induced HepG2 cells when treated with Py. (pep) in a dose-dependent manner.…”