Extraction and purification of phosphatidylcholine from soyabean lecithin

Patil, Vilas Venunath; Galge, Revanappa V.; Thorat, Bhaskar N.

doi:10.1016/j.seppur.2010.08.006

Cited by 28 publications

(20 citation statements)

References 14 publications

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“…Commercial soybean lecithin is comprised of phospholipids (PLs) bearing phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA), and phosphatidylinositol (PI), as well as sphingolipids, triglycerides, free fatty acids, and glycolipids . Soybean originated phospholipids are widely used as natural emulsifiers, wetting agents, and baking improvers , as well as dietetics, cosmetics and pharmaceuticals . With the growth of their value‐added applications, the demand for high purity PLs, specifically PC, is increasing rapidly.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous extraction of phosphatidylcholine and phosphatidylethanolamine from soybean lecithin

John

Park

Lee

et al. 2015

Euro J Lipid Sci & Tech

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A high composition of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) was simultaneously extracted from soybean lecithin by using a mixed solvent consisting of ethanol and chloroform. The addition of chloroform in ethanol improved the total extraction yield to give high a composition of PC and PE. Thus, the best extraction yield (52%) of PC and PE was achieved at an ethanol/chloroform ratio of 6/4 (v/v). Molecular dynamics calculations of the amorphous cell bearing PC, PE, and solvent molecules were performed in order to understand the distribution of PC and PE molecules in specific solvent conditions. The extraction yield was also sensitive to extraction temperature, time and liquid to solid ratio. The extracted PC and PE mixture was successfully purified by flash chromatography using chloroform/methanol (8/2 v/v) as eluting solvent. The purified yields of PE and PC from lecithin extract were 16 and 35%, respectively. The structure of the extracted products was characterized by thin-layer chromatography and 1 H, 13 C, 31 P NMR, FTIR and UV/vis spectroscopies, and elemental analysis. The purity of PE and PC was higher than 95%.Practical applications: Lecithin composed of phosphoric acid, choline, fatty acids, glycerol, glycolipids, triglycerides, and phospholipids is widely used in human food, animal feed, pharmaceuticals, paints, and other industrial applications. Lecithin can easily be extracted chemically using various solvents in order to obtain pure components. Due to the importance of phospholipids there have been a number of reports on the extraction of PC from lecithin; however, there have been few reports on the extraction of PE or the simultaneous extraction of PC and PE. Thus the simultaneous extraction of PC (35%) and PE (16%) from soybean lecithin by using a mixed solvent consisting of ethanol and chloroform can be a good example to obtain such components in high yields. The protocol employed here is quite straightforward so that pure PC and PE can be obtained economically and may attract interest on mixed solvent systems for the simultaneous extraction of lecithin components.

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Section: Introductionmentioning

confidence: 99%

Simultaneous extraction of phosphatidylcholine and phosphatidylethanolamine from soybean lecithin

John

Park

Lee

et al. 2015

Euro J Lipid Sci & Tech

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“…Lecithin is usually produced as a by-product during the refining of plant oils. The increased demand for lecithin and the considerable growth of the oil processing industry have currently made plant oils a viable source for lecithin production (Cabezas et al 2009;Patil et al 2010). Moreover, with increasing advances in biotechnology, oleaginous microorganisms have received increasing attention in the synthesis of oils, in which the PL biosynthesis pathway competes with triacylglycerol formation (Liang and Jiang 2013).…”

Section: Discussionmentioning

confidence: 99%

Enzymatic synthesis and modification of structured phospholipids: recent advances in enzyme preparation and biocatalytic processes

Hama¹,

Ogino

Kondo

2015

Appl Microbiol Biotechnol

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Phospholipids (PLs) containing specific polar head groups and fatty acids, artificially synthesized from a complex mixture of natural PLs, have considerable industrial applications. The biocatalytic approaches to synthesizing structured PLs are of great interest because the enzymes used show high selectivity and performance under mild conditions, leading to the generation of products that cannot easily be obtained by chemical catalysis. Although the limited supply of phospholipases (e.g., phospholipase D) has thus far been an obstacle to the widespread use of enzymatic processing, recent advances in enzyme preparation have opened up various applications for PL modification. In this review, attempts to increase the productivity and utility of microbial phospholipases and lipases are presented. We also summarize recent developments in enzyme-catalyzed modification of PLs, focusing particularly on the relevant reactions, bioreactor design, and novel proof-of-concept experiments.

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“…The concentration of LPC in ethanol soluble fraction was 53.2 %, which was almost three times as high as that in the original canola lysolecithin. Ethanol is the most effective solvent for enrichment of PC from lecithin produced by water degumming [13]. It is not surprising that this alcohol also shows excellent selectivity for LPC, which has only one less fatty acid than PC in its chemical structure.…”

Section: Effect Of Solvent Type On Lpc Enrichmentmentioning

confidence: 99%

“…Based on the minimum amount of solvent required to suspend the lysolecithin sample, the 2:1 ethanol/lysolecithin ratio was chosen as the lowest level of this factor. The choice for the highest level of ethanol/lysolecithin ratio (10:1) was based on the result for PC enrichment from lecithin by ethanol [13]. The range for temperature was chosen according to the capacity of the equipment available to this study and the literature [14].…”

Section: Optimization Of Lpc Enrichmentmentioning

confidence: 99%

Enrichment of Lysophosphatidylcholine in Canola Lysolecithin

Xie

Dunford

2015

J Americ Oil Chem Soc

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Lysophosphatidylcholine (LPC) possesses excellent oil‐in‐water emulsifying properties and health benefits. The objective of this study was to produce an LPC‐enriched fraction from lysolecithin generated during enzymatic degumming of crude canola oil. Three alcohols (methanol, ethanol and isopropanol) were evaluated for their effectiveness at enriching LPC. A 3 × 3 full factorial design was employed to study the effects of two processing parameters (temperature and alcohol/lysolecithin ratio) on three responses (yield and LPC concentration of alcohol soluble fraction, and LPC recovery) with the most effective alcohol. Ethanol was found to be the best solvent to enrich LPC in lysolecithin. An ethanol soluble fraction with more than 50 % LPC was produced. Quadratic models with R2 > 0.9 were developed to describe the relationship between the processing parameters and the responses in the 3 × 3 full factorial experiment. Both ethanol soluble fraction yield and LPC recovery increased with increasing temperature and ethanol/lysolecithin ratio. LPC concentration in the ethanol soluble fraction was enhanced with decreasing temperature and ethanol/lysolecithin ratio. According to the analysis, ethanol soluble fractions with LPC concentration higher than 66 % could be obtained at temperatures of 0–40 °C and an ethanol/lysolecithin ratio of 2:1 (v/w).

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Extraction and purification of phosphatidylcholine from soyabean lecithin

Cited by 28 publications

References 14 publications

Simultaneous extraction of phosphatidylcholine and phosphatidylethanolamine from soybean lecithin

Simultaneous extraction of phosphatidylcholine and phosphatidylethanolamine from soybean lecithin

Enzymatic synthesis and modification of structured phospholipids: recent advances in enzyme preparation and biocatalytic processes

Enrichment of Lysophosphatidylcholine in Canola Lysolecithin

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