Extraction, and Purification of Peroxidase Enzyme from Peganum harmala Seeds

Abstract

The aim of this study was to extract peroxides enzyme from Peganum harmala seeds; peroxides was extracted by using different extraction buffer solutions, then it was purified by three steps of purification includes precipitation with ammonium sulfate in a saturation ratio of 70 %, ion exchange chromatography through DEAE-Cellulose, and gel filtration chromatography throughout Sephadex G-100, and determine the optimum condition for extraction. This was performed by controlling the type and concentration of buffer, pH of the buffer used, and the ratio of extraction. The Sodium acetate buffer with 0.2mM and pH 5.0 was found to be the best buffer for the extraction of peroxidase. By using the extraction ratio for a plant of 1:3 (W/V), the specific activity was 195 U/mg protein. These three purification steps raised the specific activity to 235U/mg protein in the precipitation step with purification fold 2.3 and enzyme recovery 69%; the specific activity was increased to 243U/mg protein in Ion exchange step with purification fold 2.4 and enzyme recovery 23%, also the specific activity doubled after gel filtration step to 447U/mg protein with purification fold 4.4 and enzyme recovery 15%.

Effect of phytosterols on the performance, lipid metabolism, and antioxidation capacity of Sows

“…A proximate analysis of the diets was conducted by Huazhong Agricultural University (Wuhan, China). Metabolisable energy, crude protein, calcium, total phosphorus, and lysine in the experimental diets were analysed according to the guidelines of the Association of Official Analytical Chemists [27]. The nutrient compositions are dry matter basis.…”

Extraction, Purification and Characterization of Peroxidase from Okra (Abelmoschus esculentus)

“…The specific activity, enzyme activity and the yield after DEAE chromatography, were 87.5 U/mg, 14 U/ml and 33.3% respectively (Table 1). [14],Peroxidase activity was isolated from Peganum harmala seeds using the DEAE -Cellulose column, which had been pre-equilibrated with 0.2 M phosphate buffer pH 5. The enzyme's specific activity was 343 U/mg, the purification fold was 2.4, and the yield was 23%.…”

Application of Peleg model in osmotic dehydration of Smallanthus sonchifolius

“…Durante el proceso de impregnación (osmosis) a cada cierto tiempo (10, 20, 40, 60, 80, 100, 120, 150 minutos) se tomaron muestras, las cuales fueron secadas con papel absorbente para eliminar la mayo r cantidad de la solución de … adherido a la superficie de los cubos. (c) Posteriormente, se procedió a realizar el pesado de las muestra en una balanza analítica marca Sartorius y se procedió a determinar l su humedad mediante el método AOAC [12], pérdida de agua y el contenido de solidos solubles (°Brix) con un refractómetro digital marca Hanna.…”