Extraction and purification of a new compound containing selenium and mercury accumulated in dolphin liver

Ping, Liu; Nagasawa, Hiromichi; Matsumoto, Kazuko; Suzuki, Akira; Fuwa, Keiichirō

doi:10.1007/bf02795534

Cited by 11 publications

(3 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A literature review by Cuvin-Aralar and Furness (1991) presented several possible mechanisms of protection, including a redistribution or excretion of Hg in the presence of Se, a competition for binding sites between both elements, the formation of Hg-Se complexes, the conversion of toxic forms to less toxic forms of Hg, and the prevention of oxidative damage from Hg by Se through an increased activity of glutathione peroxidase. Among those mechanisms, the formation of an inorganic and (or) protein Hg-Se complex is proposed and frequently supported by analytical evidences derived by traditional chromatographic techniques (Burk et al 1974;Iwata et al 1981;Ping et al 1986), high-performance liquid chromatography (HPLC) combined with inductively coupled plasma-mass spectrometry (ICP-MS; Suzuki 1997a, 1997b;Sasakura and Suzuki 1998), microscopy (Christensen et al 1989), or X-ray techniques (Carmichael and Fowler 1980;Lindh and Johansson 1987;Gailer et al 2000). These complexes are generally found in storage organs such as liver and kidney and even in hair for humans (Soares de Campos et al 2002).…”

Section: Introductionmentioning

confidence: 92%

The effect of selenium on mercury assimilation by freshwater organisms

Belzile¹,

Chen²,

Gunn³

et al. 2006

Can. J. Fish. Aquat. Sci.

126

View full text Add to dashboard Cite

In this study, we showed that selenium (Se) deposition from metal smelters in Sudbury, Ontario, greatly reduces the bioassimilation of mercury (Hg) by aquatic biota throughout the food web. Concentrations of total and methyl mercury in tissues of zooplankton, mayflies (Stenonema femoratum), amphipods (Hyalella azteca), and youngof-the-year perch (Perca flavescens) were positively correlated with increasing distance from Sudbury smelters and inversely correlated with Se concentrations in lake water. Although concentrations of total Hg and total Se in tissues showed weak or no correlation in most of the studied aquatic species, methyl mercury and total Se showed much clearer inverse trends. Similar to the results of our previous study of muscle tissue from adult perch and walleye (Sander vitreus), these findings suggest that Se plays an important role in limiting the whole-body assimilation of Hg at lower levels of the aquatic food chain. High Se concentrations may force a preferential assimilation of the element over Hg through a competitive adsorption on binding sites. They may also restrict the solubility and availability of Hg to aquatic organisms or reduce the methylation of this metal in lakes.Résumé : Nous montrons dans cette étude que la déposition de sélénium (Se) provenant des hauts fourneaux de Sudbury en Ontario réduit grandement l'assimilation du mercure (Hg) par le biote aquatique tout au long de la chaîne alimentaire. Les concentrations en Hg total et méthylmercure dans les tissus de zooplancton, éphémères (Stenonema femoratum), amphipodes (Hyalella azteca) et jeunes perchaudes (Perca flavescens) de l'année ont pu être directement corrélées avec la distance qui les sépare des hauts fourneaux de Sudbury et inversement corrélées avec les concentrations de Se dans l'eau de lac. Alors que les concentrations en Hg total et Se total dans les tissus ne montrent que des corrélations faibles ou nulles entre elles chez la plupart des organismes étudiés, le méthylmercure et le Se total montrent des relations inverses beaucoup plus claires. De façon similaire aux résultats de notre étude précédente sur les tissus musculaires de perchaudes et de dorés (Sander vitreus) adultes, ces données laissent croire que le Se joue un rôle important en limitant l'assimilation intégrale du Hg aux niveaux inférieurs de la chaîne alimentaire aquatique. Les fortes concentrations de Se pourraient conduire à une assimilation préférentielle de cet élément sur celle du Hg à travers une adsorption compétitive sur des sites de liaison. Elles pourraient aussi restreindre la solubilité et la disponibilité du mercure chez les organismes aquatiques ou réduire la méthylation de ce métal dans les lacs.Belzile et al. 10

show abstract

Section: Introductionmentioning

confidence: 92%

The effect of selenium on mercury assimilation by freshwater organisms

Belzile¹,

Chen²,

Gunn³

et al. 2006

Can. J. Fish. Aquat. Sci.

126

View full text Add to dashboard Cite

show abstract

“…Ping et al [69] isolated a LMW compound containing Hg and Se at an equimolar ratio in the liver of striped dolphin. Although both Hg and Se existed in LMW fraction in hepatic cytosol of the three species used in the present study (Fig.…”

Section: Sephadex G-75 Chromatography Of Hepatic Cytosol Fraction In mentioning

confidence: 99%

Association of heavy metals with metallothionein and other proteins in hepatic cytosol of marine mammals and seabirds

Ikemoto

Kunito

Anan

et al. 2004

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Distribution of Cu, Zn, Cd, Ag, Hg, and Se were determined in hepatocytosol of northern fur seals (Callorhinus ursinus), black-footed albatrosses (Diomedea nigripes), and Dall's porpoises (Phocoenoides dalli). Copper, Zn, and Cd were accumulated preferentially in metallothionein (MT) fraction and their contents in MT fraction increased with the amounts in the hepatocytosol. Silver was bound to both high-molecular-weight substances (HMWS) and MT in the hepatocytosol for all three species, whereas the distribution of Ag in the cytosol was different among the three species. In northern fur seals, Ag mainly was bound to MT, whereas it mainly was associated with HMWS in Dall's porpoises. In contrast, Ag was distributed almost equally in both HMWS and MT for black-footed albatrosses. Mercury content in HMWS and Se content in HMWS and low-molecular-weight substances (LMWS) increased with their contents in hepatocytosol for all the three species. A significant positive correlation was found between Se and Hg contents in high-molecular weight (HMW) fraction in cytosol. The molar ratio of Hg and Se was close to unity in HMW fraction of the specimens with high Hg concentration in cytosol, implying that the Hg-Se complex was bound to the HMWS. Analysis of metals in the hepatocytosol by high-performance liquid chromatography/inductively coupled plasma-mass spectrometry (HPLC/ICP-MS) suggests that multiple isoforms of MT are present in hepatocytosol of the three species and that the metal profiles in hepatocytosols are different among the species. To our knowledge, this is the first report on the association of Ag with HMWS and MT in hepatocytosol of marine mammals and seabirds. Also, distribution and interaction of Hg and Se were investigated for the first time in hepatocytosol of the higher trophic marine animals.

show abstract

“…Although less so than Hg, Se can also become a toxic substance. It is thought that Se has the capacity to reduce the toxicity of inorganic Hg, forming a complex with equal proportions of the two elements [52,55,56]. This mercuric selenide can be considered as a product of the detoxification process of marine mammals and birds.…”

Section: Discussionmentioning

confidence: 99%

Trace Metal Concentrations in the Hair of Zalophus californianus Pups and their Relation to Feeding Habits

Elorriaga‐Verplancken

Aurioles‐Gamboa

2008

Biol Trace Elem Res

View full text Add to dashboard Cite

The levels of six trace metals (Hg, Se, Pb, Cu, Fe, and Zn) were measured in the hair of California sea lion pups from eight rookeries in the Gulf of California, with the aim of relating these levels with the animals feeding habits, trophic level, and delta(15)N signatures at each locality. Trace metal levels were seen to vary significantly between sample sites as were tendencies associated with feeding habits. Mercury concentrations showed a clear positive correlation with trophic level (r = 0.73, p = 0.06) as well as the delta(15)N values of the rookeries (r = 0.79, p = 0.033). This was explainable via the process of biomagnification, although other elements did not follow the same tendency. High Cu concentrations in the hair (18 and 47 microg g(-1); ANOVA, p < 0.05) were observed at San Pedro Mártir and San Esteban, where the consumption of squid is higher than at other localities (approximately 17% and approximately 27% of the diet, respectively). Se and Hg concentrations correlated positively (r = 0.73, p = 0.038), probably the result of detoxification processes, a phenomenon previously reported in marine mammals. In light of the high degree of philopatry among sea lions in the Gulf and their pups' dependence exclusively upon their mother's milk during the first months of life, the analysis of trace metals in the easily sampled hair of the pups, offers an indirect way of monitoring the toxicology of the sea lion populations.

show abstract

Extraction and purification of a new compound containing selenium and mercury accumulated in dolphin liver

Cited by 11 publications

References 18 publications

The effect of selenium on mercury assimilation by freshwater organisms

The effect of selenium on mercury assimilation by freshwater organisms

Association of heavy metals with metallothionein and other proteins in hepatic cytosol of marine mammals and seabirds

Trace Metal Concentrations in the Hair of Zalophus californianus Pups and their Relation to Feeding Habits

Contact Info

Product

Resources

About