Extraction and isolation of Pistacia lentiscus L. essential oil by supercritical CO₂

Abstract

Supercritical CO 2 extraction coupled to a fractional separation technique (SFE) was used to isolate essential oil from the leaves and berries of Pistacia lentiscus L. The technique adopted allowed us to separate the volatile oil from waxes. In the case of the leaf essential oil, the yield was 0.45% by weight of the charged material. The chemical composition of the oil, determined by GC-MS analysis, revealed the presence ofˇ-caryophyllene (31.38%), germacrene D (12.05%) and -cadinene (6.48%). Remarkable differences were noticed in the corresponding hydrodistilled oil, composed chiefly byˇ-pinene (18.71%),ˇ-phellandrene (12.83%) anď -caryophyllene (13.22%). The yield of volatile oil obtained or treating Pistacia lentiscus berries was only 0.2%.-myrcene,˛-pinene and˛-phellandrene are the compounds characteristic of the oil obtained with both methods, SFE and hydrodistillation.

Chemical Composition and Antioxidant Potential of <i>Pistacia lentiscus</i> L. Essential Oil from Oran (Algeria)

“…In our study terpinene-4-ol was by far the major component (41.24%) accompanied [9] and 31.5% Italy [20].…”

“…In other countries of the Mediterranean region, several studies have been studied the chemical composition of P. lentiscus L. oil [6]- [20] and several compositions were observed. Myrcene (39.2%), which is the major compounds of our essential oil, has also the abundant compound in the samples from Morocco (38%) [4], France (76.9%) [6], Spain (27%) [7], Italy (25.2%) [8] and Algeria (23.0% -33.1%) [9].…”

“…Some works reported the chemical composition of the essential oil from aerial parts of P. lentiscus L. of diverse countries of the Mediterranean region [6]- [20]. The chemical composition of the essential oil derived from the aerial parts is not clear; it is greatly influenced by both geographical origin and isolation technique.…”

Extraction, separation and isolation of essential oils from natural matrices by supercritical CO2

“…On the basis of previous studies [22][23] we chose the following extraction conditions: 90 bar and 50°C (density of carbon dioxide, ρCO 2 = 0.287 g/cm 3 ). These conditions allowed the extraction of volatile oil without undesired compounds, except waxes, for both treated materials.…”

Chemical composition of the essential oil of Pistacia lentiscus L. from Morocco—a seasonal variation

“…An essential oil has been isolated from P. lentiscus by supercritical CO 2 . 7 Many authors have found that the P. lentiscus essential oil has interesting antibacterial and antimicrobial activity. 8,9 Marone et al, 10 evaluating the antibacterial activity of P. lentiscus mastic gum against Helicobacter pylori, have found that the mastic gum has a very interesting activity.…”