Extraction and in vitro screening of potential acetylcholinesterase inhibitors from the leaves of Panax japonicus

Li, Sainan; Liu, Chengyu; Liu, Chunming; Zhang, Yuchi

doi:10.1016/j.jchromb.2017.07.019

Cited by 19 publications

(18 citation statements)

References 25 publications

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“…Five new ginsenosides that are derivatives of ursolic acid have been isolated from Panax japonicus (37). All five were identified as AChE inhibitors in an evaluation of AChE activity in a PC12 cell model (rat adrenal pheochromocytoma) that was treated with Aβ 25-35 .…”

Section: Ginsenosides and The Acetylcholine Levelmentioning

confidence: 99%

Panax ginseng components and the pathogenesis of Alzheimer's disease (Review)

et al. 2019

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Ginseng is one of the main representatives of traditional Chinese medicine and presents a wide range of pharmacological actions. Ginsenosides are the main class of active compounds found in ginseng. They demonstrate unique biological activity and medicinal value, namely antitumour, anti-inflammatory and antioxidant properties, as well as anti-apoptotic properties. Increasing levels of stress in life are responsible for the increased incidence of nervous system diseases. Neurological diseases create a huge burden on the lives and health of individuals. In recent years, studies have indicated that ginsenosides play a pronounced positive role in the prevention and treatment of neurological diseases. Nevertheless, research is still at an early stage of development, and the complex mechanisms of action involved remain largely unknown. This review aimed to shed light into what is currently known about the mechanisms of action of ginsenosides in relation to Alzheimer's disease. Scientific material and theoretical bases for the treatment of nervous system diseases with purified Panax ginseng extracts are also discussed.

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Section: Ginsenosides and The Acetylcholine Levelmentioning

confidence: 99%

Panax ginseng components and the pathogenesis of Alzheimer's disease (Review)

et al. 2019

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“…According to sapogenin structure, these saponins can be divided into four types, which were protopanaxadiol (PPD), protopanaxatriol (PPT), oleanolic acid (OA) and ocotillone (OC) and mostly exhibited fragmentation ways corresponding to losses of H 2 O, CO 2 , sugars and other substituents until the formation of characteristic product ions. PPD type of saponins (3,5,11,19,21,22,26,30,35,38,41,42,43,46,47,48, 50 and 52 in Table 2) possessed a characteristic product ion at 459, for example, ginsenoside Rg 3 (20R) (Fig. 4A), the fragment ion 459.3814 of which can be considered as the losses of 324 Da (Glc+Glc) (Glc: β-D-glucose).…”

Section: Resultsmentioning

confidence: 99%

Qualitative and Quantitative Analysis of the Saponins in Panacis Japonici Rhizoma Using Ultra-Fast Liquid Chromatography Coupled with Triple Quadrupole-Time of Flight Tandem Mass Spectrometry and Ultra-Fast Liquid Chromatography Coupled with Triple Quadrupole-Linear Ion Trap Tandem Mass Spectrometry

et al. 2019

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Panacis Japonici Rhizoma (PJR) contains various kinds of saponins, which possesses extensive pharmacological activities, but studies of comprehensive analysis of its saponins were limited. Thus, ultra-fast liquid chromatography coupled with triple quadrupole-time of flight tandem mass spectrometry (UFLC-Triple TOF-MS/MS) and ultra-fast liquid chromatography coupled with triple quadrupole-linear ion trap tandem mass spectrometry (UFLC-QTRAP-MS/MS) methods were established for the qualitative and quantitative analysis of the saponins in PJR, separately. Fifty three saponins in PJR were identified by UFLC-Triple TOF-MS/MS method, 23 saponins of which were unequivocally identified by reference substances. In addition, fragmentation pathways of different types of saponins were preliminarily deduced by fragmentation behavior of 53 saponins. Furthermore, the simultaneous determination of the contents of 13 saponins in PJR samples harvested at different times were analyzed by UFLC-QTRAP-MS/MS method. Furthermore, the quality of the samples was evaluated by grey relational analysis. This study might be beneficial to the quality assessment and control of PJR. Meanwhile, it might provide the basic information for confirming its optimal harvested period. Key words Panacis Japonici Rhizoma; saponin; different harvesting time; ultra-fast liquid chromatography coupled with triple quadrupole-time of flight tandem mass spectrometry (UFLC-Triple TOF-MS/MS); UFLC-triple quadrupole-linear ion trap tandem (QTRAP)-MS/MS

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“…Ginseng is one of the most revered medicinal plant in traditional oriental medicine [ 1 ]. Different medicinal applications of ginseng, including anti-Alzheimer disease activity [ 2 ], anticancer properties [ 3 ] and treatment of the skin diseases, e.g. atopic dermatitis [ 4 ], are often associated with its active components – ginsenosides.…”

Section: Introductionmentioning

confidence: 99%

Quantitative aspects of the hydrolysis of ginseng saponins: Application in HPLC-MS analysis of herbal products

Abashev

Stekolshchikova

Ставрианиди

2021

Journal of Ginseng Research

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Background Ginseng is one of the most valuable herbal supplements. It is challenging to perform quality control of ginseng products due to the diversity of bioactive saponins in their composition. Acid or alkaline hydrolysis is often used for the structural elucidation of these saponins and sugars in their side chains. Complete transformation of the original ginsenosides into their aglycones during the hydrolysis is one of the ways to determine a total saponin group content. The main hurdle of this approach is the formation of various by-products that was reported by many authors. Methods Separate HPLC assessment of the total protopanaxadiol, protopanaxatriol and ocotillol ginsenoside contents is a viable alternative to the determination of characteristic biomarkers of these saponin groups, such as ginsenoside Rf and pseudoginsenoside F 11 , which are commonly used for authentication of P. ginseng Meyer and P. quinquefolius L. samples respectively. Moreover, total ginsenoside content is an ideal aggregated parameter for standardization and quality control of ginseng-based medicines, because it can be directly applied for saponin dosage calculation. Results Different hydrolysis conditions were tested to develop accurate quantification method for the elucidation of total ginsenoside contents in herbal products. Linearity, limits of quantification, limits of detection, accuracy and precision were evaluated for the developed HPLC-MS method. Conclusion Alkaline hydrolysis results in fewer by-products than sugar elimination in acidic conditions. An equimolar response, as a key parameter for quantification, was established for several major ginsenosides. The developed approach has shown acceptable results in the analysis of several different herbal products.

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Extraction and in vitro screening of potential acetylcholinesterase inhibitors from the leaves of Panax japonicus

Cited by 19 publications

References 25 publications

Panax ginseng components and the pathogenesis of Alzheimer's disease (Review)

Panax ginseng components and the pathogenesis of Alzheimer's disease (Review)

Qualitative and Quantitative Analysis of the Saponins in Panacis Japonici Rhizoma Using Ultra-Fast Liquid Chromatography Coupled with Triple Quadrupole-Time of Flight Tandem Mass Spectrometry and Ultra-Fast Liquid Chromatography Coupled with Triple Quadrupole-Linear Ion Trap Tandem Mass Spectrometry

Quantitative aspects of the hydrolysis of ginseng saponins: Application in HPLC-MS analysis of herbal products

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