Extraction and determination of papaverin in pericarpium papaveris using aqueous two-phase system of poly(ethylene glycol)–(NH4)2SO4 coupled with high-performance liquid chromatography

Cao, Qing; Li, Shehong; He, Chiyang; Li, Kèan; Liu, Feng

doi:10.1016/j.aca.2007.03.050

Cited by 32 publications

(26 citation statements)

References 21 publications

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“…Several tens nanometer size and PEG shell layer might prevent internalization in healthy blood cells. PEG, which is the hydrophilic segment of PEG- b -PMOT, is one of the most widely used polymers as remarkable biocompatible materials, (30) which possesses the ability to repel biomolecules, including cell surfaces, due to its strong hydration ability, (31) conformational ﬂexibility, (32) and repulsive force of the PEG tethered chain. (33) In addition, size of several nanometers reduces the permeability of RNP O , which is in sharp contrast to the LMW compound.…”

Section: Resultsmentioning

confidence: 99%

The behavior of ROS-scavenging nanoparticles in blood

Shimizu

Yoshitomi

Nagasaki

2014

J. Clin. Biochem. Nutr.

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Here, we report an interaction between blood and redox nanoparticles, prepared by self-assembly of amphiphilic block copolymers possessing 2,2,6,6-tetramethylpiperidine-N-oxyls as a side chain of hydrophobic segment. When 4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl was added to rat whole blood, its electron spin resonance signal disappeared rapidly. In contrast, the signal from redox nanoparticles remained for a long period of time, indicating that nitroxide radicals were protected in the blood by their compartmentalization in the core of nanoparticle. Although most 2,2,6,6-tetramethylpiperidine-N-oxyls were located in the nanoparticle core, reactive oxygen species-scavenging activity was found outside of blood cells. For example, redox nanoparticles suppressed superoxide anion-induced hemolysis effectively, while 4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl did not. It was revealed that redox nanoparticles were not internalized into the healthy blood cells, which was in sharp contrast to 4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl. Due to its internalization into healthy platelets, 4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl induced mitochondrial dysfunction, while redox nanoparticles did not. Redox nanoparticles suppressed platelet adhesion and extended blood coagulation time, in contrast to 4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl. These results indicate that redox nanoparticles scavenge reactive oxygen species outside of cells, but do not interfere with normal redox reactions inside of the cell. Based on these results, we determine that an anti-oxidative strategy based on nanotechnology is a rational and safe therapeutic approach.

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Section: Resultsmentioning

confidence: 99%

The behavior of ROS-scavenging nanoparticles in blood

Shimizu

Yoshitomi

Nagasaki

2014

J. Clin. Biochem. Nutr.

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“…It has recently been suggested that an optimized ATPS can serve as a pre‐sorting stage in proteomic studies since they can be customized for the selective extraction and/or partition of large fractions of proteins from crude extracts 67. Coupling ATPSs to traditional chromatographic techniques has proved to be a powerful technique for the purification of proteins from plants 11, 68. Combined with a high‐performance liquid chromatography (HPLC), a primary extraction using an ATPS was successfully applied to the determination of bioactive alkaloids from Pericarpium papaveris , a Chinese herb, reaching detection limits of 2 ng mL −1 .…”

Section: Coupling Aqueous Two‐phase Systems To Traditional Operationsmentioning

confidence: 99%

“…Combined with a high‐performance liquid chromatography (HPLC), a primary extraction using an ATPS was successfully applied to the determination of bioactive alkaloids from Pericarpium papaveris , a Chinese herb, reaching detection limits of 2 ng mL −1 . Particularly for this application, the main advantage of this method when compared with traditional liquid–liquid or solid‐phase extraction was simplicity due to the direct injection of the upper PEG‐rich phase into the HPLC system 68. For most of the cases, dialysis of the recovered phase is the common method prior to a chromatographic separation for removal of the phase‐forming components, a feature that may hamper the coupling of chromatography and ATPS for large‐scale operations.…”

Section: Coupling Aqueous Two‐phase Systems To Traditional Operationsmentioning

confidence: 99%

“…67 Coupling ATPSs to traditional chromatographic techniques has proved to be a powerful technique for the purification of proteins from plants. 11,68 Combined with a highperformance liquid chromatography (HPLC), a primary extraction using an ATPS was successfully applied to the determination of bioactive alkaloids from Pericarpium papaveris, a Chinese herb, reaching detection limits of 2 ng mL −1 . Particularly for this application, the main advantage of this method when compared with traditional liquid-liquid or solid-phase extraction was simplicity due to the direct injection of the upper PEGrich phase into the HPLC system.…”

Section: Coupling Aqueous Two-phase Systems To Traditional Operationsmentioning

confidence: 99%

See 1 more Smart Citation

Aqueous two-phase systems strategies for the recovery and characterization of biological products from plants

Aguilar

Rito‐Palomares

2010

J. Sci. Food Agric.

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The increasing interest of the biopharmaceutical industry to exploit plants as economically viable production systems is demanding the development of new downstream strategies to maximize product recovery. Aqueous two-phase systems (ATPSs) are a primary recovery technique that has shown great potential for the efficient extraction and purification of biological compounds. The present paper gives an overview of the efficient use of ATPS-based strategies for the isolation and partial purification of bioparticles from plant origin. Selected examples highlight the main advantages of this technique, i.e. scaling-up feasibility, process integration capability and biocompatibility. An overview of the recent approach of coupling ATPSs with traditional techniques to increase bioseparation process performance is discussed. A novel approach to characterization protein from plants combining ATPSs and two-dimensional electrophoresis (2-DE) is introduced as a tool for process development. In the particular case of products from plant origin, early success has demonstrated the potential application of ATPS-based strategies to address the major disadvantages of the traditional recovery and purification techniques. This literature review discloses the relevant contribution of ATPSs to facilitate the establishment of bioprocesses in the growing field of high-value products from plants.

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“…PEG is often used in aqueous two-phase partitioning studies [22,[25][26][27][28], and there are extensive data in literature reporting the use of this polymer. Poly(propylene glycol) 400 (PPG) is a polymer that is structurally closely related to PEG.…”

Section: Introductionmentioning

confidence: 99%