Extraction and clean-up methods for organochlorine pesticides determination in milk

Martins, Joana; Amaya-Chávez, Araceli; Waliszewski, Stefan M.; Cruz, Alejandro Delgado; García-Fábila, María Magdalena

doi:10.1016/j.chemosphere.2013.04.008

Cited by 58 publications

(27 citation statements)

References 76 publications

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“…De acuerdo a las concentraciones de endosulfán en la última década (cuadro I) los productos lácteos que sobrepasaron el límite máximo de residuos (LMR) de 0.01 mg/kg de grasa de acuerdo al Codex Alimentarius (Fernícola 1985, Gomes et al 2013) fueron el yogurt, la leche bovina, la leche de cabra y la manteca de leche de vaca (Lorenzatti et al 2003, Prado et al 2007, Medina et al 2010y Castilla-Pinedo et al 2012; la ONU marca un LMR en leche de 0.004 mg/kg en base grasa con esa referencia la leche materna se incluye entre los alimentos que sobrepasan este límite (Campoy et al 2001, Cerrillo et al 2005, Rodas-Ortiz et al 2008, Freguglia et al 2011. La mayor presencia de sulfato de endosulfán indica una exposición anterior, mientras que una mayor concentración de los isómeros α y β, refiere una exposición reciente a este plaguicida (Campoy et al 2001, Cerrillo et al 2005.…”

Section: Productos Lácteosunclassified

“…Presentan características lipofílicas con alta persistencia y resistencia a la degradación, con una vida media en humanos cercana a los 10 años (Castilla et al 2012, Gomes et al 2013. Los POC son considerados disruptores endocrinos, precursores de carcinogénesis en roedores, causantes de linfoma de non-Hodgkin's; inducen hepatotoxicidad e inmunotoxicidad, además de efectos en el neurocomportamiento (Gomes et al 2013). Tienen, la capacidad de acumularse en el tejido adiposo de animales y de biomagnificarse a través de la cadena trófica.…”

Section: Introductionunclassified

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Métodos Cromatográficos Para La Determinación De Endosulfán en Alimentos

Ángeles-Hernández

Amaya-Chávez

Rivera

et al. 2018

Rev. Int. Contam. Ambie.

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Palabras clave: plaguicidas orgánicos persistentes, extracción y limpieza, metodología analítica RESUMEN Se revisaron las técnicas de extracción, limpieza y cuantificación de endosulfán por métodos cromatográficos en matrices alimentarias como productos lácteos, frutas y vegetales, así como pescados y mariscos, publicados en los últimos veinte años. Independientemente del tipo de matriz, su extracción se realiza principalmente con disolventes orgánicos como hexano, metanol, acetonitrilo y acetona. Para la limpieza, lo más comúnmente empleado fueron cartuchos de sílice, columnas de florisil, o C18. En cuanto a la cuantificación, el método más sensible resultó ser la cromatografía de gases acoplada a espectrofotometría de masas (CG-MS). Los alimentos en los que se reportó la presencia de α, β y/o sulfato de endosulfán fueron: productos lácteos y sus derivados con un intervalo de concentraciones de 23.99 a 52.2 ng/g; pescados y mariscos de 1.07 a 49.7 ng/g; y vegetales de 0.5 a 5460.0 ng/g. En leche materna se reportaron concentraciones de 0.4 a 10.7 ng/mL y en grasa hasta 277.39 ng/g. Keywords: persistent organic pesticides, extraction and cleaning, analytic methodology ABSTRACT Extraction and cleaning techniques plus chromatographic methods were reviewed for the quantification of endosulfan in food matrices such as dairy products, fruits and vegetables, fish and seafood, published in the last twenty years. Regardless of the type of matrix, the extraction of endosulfan is carried out mainly with organic solvents such as hexane, methanol, acetonitrile and acetone. For cleaning, the most common was the use silica cartridges, florisil columns, or C18. The most sensitive quantification method was gas chromatography coupled to mass spectrometry (GC-MS. Foods where it was reported the presence of α, β and/or endosulfan sulfate were dairy products and their derivatives in a range of 23.99 to 52.2 ng/g, in fish and shellfish from 1.07 to 49.7 ng/g, vegetables from 0.5 to 5460.0 ng/g. In breast milk were from 0.4 to 10.7 ng/mL and in fat until 277.39 ng/g. REVISIÓNRev. Int. Contam. Ambie. 34 (Especial sobre Contaminación y Toxicología por Plaguicidas (CTP) [81][82][83][84][85][86][87][88][89][90][91][92][93][94] 2018

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Section: Productos Lácteosunclassified

Section: Introductionunclassified

Métodos Cromatográficos Para La Determinación De Endosulfán en Alimentos

Ángeles-Hernández

Amaya-Chávez

Rivera

et al. 2018

Rev. Int. Contam. Ambie.

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“…Clean-up is an analytical procedure involving series of steps in which the bulk of the potentially interfering compounds are removed by physical or chemical methods. Various clean-up approaches are involved to eliminate the matrix effect in milk samples (Tölgyesi et al, 2012;Xia et al, 2010, Martins et al, 2013. Especially, certain clean-up procedures were successfully employed for eliminating the matrix effect during the analysis of pesticide residues in milk samples (Spinks et al, 2001;Di Muccio et al, 1997;Di Muccio et al, 1996).…”

Section: Matrix Effect and Clean-up Proceduresmentioning

confidence: 99%

Development of ELISA kit for the assay of ichlorodiphenyltrichloroethane in milk and milk products

Kantharimuthu¹,

Balaraman²,

Sivanesan³

2016

Afr. J. Food Sci.

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Immunoassay of pesticide residues in foods is gaining importance in the past two decades. The objective of the present work is to develop an enzyme -linked immunosorbent assay (ELISA) kit for the assay of dichlorodiphenyltrichloroethane (DDT) in milk and milk products. Here, an ELISA method was developed using rabbit polyclonal antibodies against a hapten, DDT-OH for the detection of organochloride pesticide DDT in milk and milk products. The immunogen for raising the antiserum was prepared from BIS (4-chlorophenyl) ethanol and succinic anhydride coupled with bovine serum albumin (BSA) One ml of immunogen was inoculated subcutaneously in multiple sites of thigh region in rabbits following three boosters at weekly intervals and the antiserum was harvested after the second booster. The ELISA response of the anti DDT antiserum, diluted and undiluted was studied against the antigen (DDT) coated onto the immuno module. The homogenous response of the anti DDT anti serum was adequate to identify DDT. Fifteen samples each of milk, buttermilk, cheese, kalakhand and khoa were quantitatively analyzed for DDT using ELISA as well as GC methods. DDT was present only below detectable limit (BDL) (5 ng g -1 ) in milk and milk products obtained from organized dairies in and around Chennai city, India. The percentage recovery was above 70% ensuring that the ELISA method developed for DDT is dependable. The developed ELISA method can be helpful in detecting the residual amount of DDT present in milk and milk products. The analysis of pesticides in samples by ELISA and GC was comparable.

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“…HS mode was used for extraction of organochlorine pesticides in wine using dodecylsulfate-doped polypyrole (Ppy-DS) fiber (Korba et al, 2013). Selection of operating in HS or in DI mode depends on the volatility of the analytes and the matrix characteristics (Martins et al, 2013).…”

Section: Sampling Modementioning

confidence: 99%

Recent achievements and applications of solid – phase microextraction in pesticide residues analysis in food

Andraščíková

Hrouzková

2015

Acta Chimica Slovaca

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Abstract:The current trend in sample preparation methods is devoted to minimizing or eliminating the volume of extractive solvent. In this review, the focus on solid phase microextraction (SPME) as a solvent free sample preparation method for the isolation of pesticides residues in different food matrices is given. To achieve satisfactory extraction efficiency, selection of the fiber coating is an important step in the method development. Here, recent trends in new supporting materials and new fiber coatings development are discussed. Finally, applicability of SPME for the pesticide residues analysis in various food matrices using mainly chromatographic methods is also reviewed.

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Extraction and clean-up methods for organochlorine pesticides determination in milk

Cited by 58 publications

References 76 publications

Métodos Cromatográficos Para La Determinación De Endosulfán en Alimentos

Métodos Cromatográficos Para La Determinación De Endosulfán en Alimentos

Development of ELISA kit for the assay of ichlorodiphenyltrichloroethane in milk and milk products

Recent achievements and applications of solid – phase microextraction in pesticide residues analysis in food

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