Extraction and characterization of xylan from sugarcane tops as a potential commercial substrate

Khaire, Kaustubh Chandrakant; Sharma, Kananbala; Thakur, Abhijeet; Moholkar, Vijayanand S.; Goyal, Arun

doi:10.1016/j.jbiosc.2021.01.009

Cited by 21 publications

(7 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The hemicellulose of SB contains 55.2 (%, w/w) xylose and 10.7 (%, w/w) arabinose of the total hemicellulose content . The enzyme xylanase (endolytically) and xylosidase (exolytically) act on the main chain of xylan polysaccharides. − The use of accessory enzymes, such as α- l -arabinofuranosidase, to hydrolyse side chains synergistically acts and enhances the rate of overall hydrolysis and increases the production of monosaccharides . The monosaccharides, arabinose, and xylose act as antiglycemic agents and are of immense importance to the human health.…”

Section: Introductionmentioning

confidence: 99%

Two-Step Saccharification of the Xylan Portion of Sugarcane Waste by Recombinant Xylanolytic Enzymes for Enhanced Xylose Production

et al. 2021

Self Cite

View full text Add to dashboard Cite

Sugarcane bagasse (SB) and sugarcane trash (SCT) containing 30% hemicellulose content are the waste from the sugarcane industry. Hemicellulose being heterogeneous, more complex, and less abundant than cellulose remains less explored. The optimized conditions for the pretreatment of SB and SCT for maximizing the delignification are soaking in aqueous ammonia (SAA), 18.5 wt %, followed by heating at 70 °C for 14 h. The optimization of hydrolysis of SAA pretreated (ptd) SB and SCT by the Box–Behnken design in the first step of saccharification by xylanase ( Ct Xyn11A) and α- l -arabinofuranosidase ( Ps GH43_12) resulted in the total reducing sugar (TRS) yield of xylooligosaccharides (TRS (XOS) ) of 93.2 mg/g ptd SB and 85.1 mg/g ptd SCT, respectively. The second step of saccharification by xylosidase ( Bo GH43) gave the TRS yield of 164.7 mg/g ptd SB and 147.2 mg/g ptd SCT. The high-performance liquid chromatography analysis of hydrolysate obtained after the second step of saccharification showed 69.6% xylan-to-xylose conversion for SB and 64.1% for SCT. This study demonstrated the optimization of the pretreatment method and of the enzymatic saccharification by recombinant xylanolytic enzymes, resulting in the efficient saccharification of ptd hemicellulose to TRS by giving 73.5% conversion for SB and 71.1% for SCT. These optimized conditions for the pretreatment and saccharification of sugarcane waste can also be used at a large scale.

show abstract

Section: Introductionmentioning

confidence: 99%

Two-Step Saccharification of the Xylan Portion of Sugarcane Waste by Recombinant Xylanolytic Enzymes for Enhanced Xylose Production

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…95 The appearance of this signal is contrary to reports where the lack of this is reported due to the saponification of the ester compounds when using alkaline pretreatments. 96 Additionally, the water bending mode (1600 cm À1 ), 97 CH 2 bending related to the xylan ring 98 and symmetric stretching vibrations of glucuronic acid groups 99 (1465 and 1413 cm À1 ), CH vibrations of carbohydrates on the rings (1370 cm À1 ), 39 CO bending (1335 cm À1 ), OH bending + carboxylic acid vibrations of glucuronic acid side group (1242 cm À1 ), 31 C-O-C stretching of glycosidic linkages of xylans (1105, 1081, 1015 cm À1 ), 100 pyranose rings of glucose, galactose, and mannose of galactoglucomannans (890 cm À1 ), 101 C-C stretching of pyranose ring (780 cm À1 ) at the anomeric region (700-950 cm À1 ), 102 were identified in the spectrum. These main functional groups suggest that the removed fraction from liquors consists of the hemicellulose biopolymer.…”

Section: Identification Of Functional Groups In Valorized Hemicellulo...mentioning

confidence: 99%

Valorization of sawdust biomass for biopolymer extraction via green method: Comparison with conventional process

Cárdenas‐Zapata

Palma‐Ramírez

Flores‐Vela

et al. 2022

Intl J of Energy Research

View full text Add to dashboard Cite

Summary The valorization of sawdust (SW) biomass by‐product, to extract cellulose through alkali and bleaching processes, including side‐streams to recover hemicellulose and lignin, is performed with the intention of evaluating differences with a proposed green method (GM). The study is developed with the intention of having a process at laboratory scale without leading to the complicated nanoscale processing. This study contemplates a process with scalable characteristics for the future, which contributes to wood processing waste around the world. Particularly, agroindustrial wastes from Durango (SWT1) and Hidalgo (SWT2), local communities of Mexico, were studied. SWT1 and SWT2 samples were processed with ethanol, sodium hydroxide followed by bleaching with sodium chlorite/acetic acid to remove, extractives (E), hemicellulose (H) and lignin (L), respectively. Selected‐SWT2 was processed through a proposed GM using hydrogen peroxide/sodium hydroxide. On the basis of results including Fourier transform infrared spectroscopy, thermogravimetric/differential analysis (TGA/DTG), X‐ray diffraction (XRD), dynamic light scattering, high heating value, scanning electron microscopy techniques and chemical composition, GM was capable of producing a biomass rich in α‐cellulose (58.0%: SWT2‐C), whereas processed SWT1 and SWT2 through conventional method led to a more percentage of H, β‐ and γ‐C mixtures than α‐polymorphous; 41.0% and 51.0%, respectively. This new method could impact of an important manner to the development of biodegradable thermoplastics. The approximation, from deconvolution of TGA/DTA, led to determine adequately the composition of hemicellulose (SWT1‐C: 51.0%, SWT2‐C: 36.0–0.00%), lignin (SWT1‐C: 17.0%, SWT2‐C: 20.0%) and cellulose (SWT1‐C: 32.0%, SWT2‐C: 44.0%). XRD analysis also indicated that samples contain the following percentage of polymorphous cellulose compounds: SWT1‐C (Type II: 71.3%), SWT2‐C (Type I α: 44.6% and II: 41.4%) and SWT2‐C‐GM (Type II: 38.8%), which confirms that the combination of the type of biomass and the applied pretreatment plays a fundamental role for final applications; in this case, it can be appropriate for elastomers and thermoplastic uses. As a final point, the cellulose composition and thermal stability of both sources is comparable with the commonly used raw material for commercial products.

show abstract

“…Xylan, the second most abundant heteropolysaccharide in nature, was the main component of hemicellulose (Schell et al, 2016). The xylan from lignocellulosic biomass was connected by β-1,4-xylosidic bonds to form a backbone, and was further covered by 4-O-methyl-D-glucuronic acid groups, acetyl groups, and α-arabinofuranose side chain group modification (Sha et al, 2020;Khaire et al, 2021). At present, xylan was mainly used to degrade to produce xylose, which was then chemically or biologically converted into biofuels, furfural, xylitol, etc.…”

Section: Xylanmentioning

confidence: 99%

Isolation of Xylanase Producing Strains, Optimization of Fermentation Conditions and Research on Enzymatic Properties

Huilong¹,

Gao²,

Wenxuan³

et al. 2021

JBLS

View full text Add to dashboard Cite

In this study, we successfully isolated a strain of Aspergillus oryzae TR08, which produced xylanase secreted to the outside of the cell productively. The enzyme activity and specific activity in the fermentation broth of this strain reached peak values of 451 IU/mL and 1963 IU/mg after 156 h of fermentation. A single factor experiment was designed, and it was found that the strain was adjusted to the initial pH of the fermentation broth to 7.5 in a shaker at 180 rpm and 32 °C. After 156 h of fermentation, the enzyme activity reached a maximum of 1264 IU/mL. The optimal reaction temperature and pH value of the xylanase were 55 °C and 7.5, respectively, and it had excellent acid and alkali resistance and a wide pH activity range. The xylanase was increased the catalytic activity by 15% in 0.25 mM Fe3+, and the biological activity of the enzyme was not affected in the sodium dodecyl sulfate environment.

show abstract

Extraction and characterization of xylan from sugarcane tops as a potential commercial substrate

Cited by 21 publications

References 44 publications

Two-Step Saccharification of the Xylan Portion of Sugarcane Waste by Recombinant Xylanolytic Enzymes for Enhanced Xylose Production

Two-Step Saccharification of the Xylan Portion of Sugarcane Waste by Recombinant Xylanolytic Enzymes for Enhanced Xylose Production

Valorization of sawdust biomass for biopolymer extraction via green method: Comparison with conventional process

Isolation of Xylanase Producing Strains, Optimization of Fermentation Conditions and Research on Enzymatic Properties

Contact Info

Product

Resources

About