Extraction and characterization of lipopolysaccharide from Pseudomonas pseudomallei

Kawahara, K

doi:10.1016/0378-1097(92)90392-2

Cited by 22 publications

(39 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The LPS isolated from B. pseudomallei has characteristics different from those of LPSs from other gram-negative bacteria. For instance, B. pseudomallei LPS has been reported to possess weaker pyrogenic activity in rodents than enterobacterial LPS but stronger mitogenic activity in murine splenocytes (21,34). A previous study by our group showed that B. pseudomallei LPS exhibits weaker and slower activation kinetics for mouse macrophage cell lines (31).…”

Section: Discussionmentioning

confidence: 89%

See 1 more Smart Citation

Fate of a Burkholderia pseudomallei Lipopolysaccharide Mutant in the Mouse Macrophage Cell Line RAW 264.7: Possible Role for the O-Antigenic Polysaccharide Moiety of Lipopolysaccharide in Internalization and Intracellular Survival

et al. 2007

View full text Add to dashboard Cite

Burkholderia pseudomallei is a facultative intracellular gram-negative bacterium that can survive and multiply inside macrophages. One of the mechanisms by which B. pseudomallei escapes macrophage killing is by interfering with the expression of inducible nitric oxide synthase (iNOS). However, the bacterial components that modulate antimicrobial activity of the macrophage have not been fully elucidated. In the present study, we demonstrated that B. pseudomallei strain SRM117, a lipopolysaccharide (LPS) mutant that lacks the Oantigenic polysaccharide moiety, was more susceptible to macrophage killing during the early phase of infection than the parental wild-type strain (1026b). Unlike the wild type, the LPS mutant could readily stimulate Y701-STAT-1 phosphorylation (pY701-STAT-1) and interferon-regulatory factor 1 (IRF-1) expression, both of which are essential transcription factors of iNOS. Neutralizing antibody against beta interferon was able to inhibit the phosphorylation of Y701-STAT-1 and the expression of IRF-1 and iNOS, all of which resulted in an increased rate of intracellular replication. These data suggest that the O-antigenic polysaccharide moiety of B. pseudomallei modulates the host cell response, which in turn controls the intracellular fate of B. pseudomallei inside macrophages.

show abstract

Section: Discussionmentioning

confidence: 89%

“…The results showed cytochalasin D to have no effect on the macrophage response. lipid A moiety, which may influence its macrophage activation activity (21). It is also possible that its O-antigen side chain might be interfering with these biological activities.…”

Section: Discussionmentioning

confidence: 99%

Fate of a Burkholderia pseudomallei Lipopolysaccharide Mutant in the Mouse Macrophage Cell Line RAW 264.7: Possible Role for the O-Antigenic Polysaccharide Moiety of Lipopolysaccharide in Internalization and Intracellular Survival

et al. 2007

View full text Add to dashboard Cite

show abstract

“…The inability of this gram-negative bacterium to stimulate IFN-␤ may be due to the unique structure of B. pseudomallei LPS. It was reported that LPS isolated from this bacterium exhibited an unusual chemical structure in the acidstable inner core region attached to the lipid A moiety which might lead to its weak macrophage activation activity (15,26). Whether B. pseudomallei LPS is an agonist for TLR2 or TLR4 remains to be investigated.…”

Section: Discussionmentioning

confidence: 99%

“…B. pseudomallei strain 844 (arabinose-negative strain) was originally isolated from a patient admitted to Srinagarind Hospital in the Khon Kaen province of Thailand, in which melioidosis is endemic. The bacterium was originally identified as B. pseudomallei based on its biochemical characteristics, colonial morphology on selective media, antibiotic sensitivity profiles, and reaction with polyclonal antibody (1,15,29) and was used in previous studies (16,27). Salmonella enterica serovar Typhi, used for comparison throughout these exper-iments, was maintained at Ramathibodi Hospital (Mahidol University, Bangkok, Thailand) and kept as a stock culture in our laboratory.…”

Section: Methodsmentioning

confidence: 99%

Involvement of Beta Interferon in Enhancing Inducible Nitric Oxide Synthase Production and Antimicrobial Activity of Burkholderia pseudomallei- Infected Macrophages

et al. 2003

View full text Add to dashboard Cite

Burkholderia pseudomallei is the causative agent of melioidosis, a life-threatening disease that affects both humans and animals. This bacterium is able to survive and multiply inside both phagocytic and nonphagocytic cells. We recently reported that mouse macrophages infected with B. pseudomallei fail to produce a significant level of inducible nitric oxide synthase (iNOS), a crucial enzyme needed for the cells to control the intracellular growth of this bacterium. In the present study, we extended our investigation to demonstrate that, unlike other gram-negative bacteria that have been investigated, B. pseudomallei only minimally activates beta interferon (IFN-␤) production; this minimal activation leads to a low level of interferon regulating factor 1 (IRF-1) in the macrophages, in parallel with poor iNOS expression. Adding exogenous IFN-␤ to the system could upregulate IRF-1 production, which in turn could enhance iNOS expression in the B. pseudomallei-infected macrophages and lead to suppression of the intracellular growth of this bacterium. Taken together, these results imply that the failure of macrophages to successfully control the growth and survival of intracellular B. pseudomallei is related, at least in part, to the defective production of IFN-␤, which modulates the ability of macrophages to synthesize iNOS.

show abstract

“…B. pseudomallei strain 844 (arabinose-negative strain) was isolated from a patient admitted to Srinakarind Hospital in the melioidosis endemic Khon Kaen province of Thailand. The bacterium was originally identified as B. pseudomallei based on its biochemical characteristics, colonial morphology on selective media, antibiotic sensitivity profiles and reaction to polyclonal antibody (1,9,22). Escherichia coli and Salmonella typhi used for comparison throughout these experiments was maintained at Ramathibodi Hospital (Mahidol University, Bangkok, Thailand) and kept as stock culture in our laboratory.…”

Section: Methodsmentioning

confidence: 99%

Burkholderia pseudomallei Interferes with Inducible Nitric Oxide Synthase (iNOS) Production: A Possible Mechanism of Evading Macrophage Killing

Utaisincharoen

Tangthawornchaikul

Kespichayawattana

et al. 2001

Microbiology and Immunology

114

View full text Add to dashboard Cite

Burkholderia pseudomallei is a causative agent of melioidosis, a life threatening disease which affects humans and animals in tropical and subtropical areas. This bacterium is known to survive and multiply inside cells such as macrophages. The mechanism of host defense against this bacterium is still unknown.In this study, we demonstrated that B. pseudomallei exhibited unique macrophage activation activity compared with Escherichia coli and Salmonella typhi, The mouse macrophage cell line (RAW 264.7) infected with B. pseudomallei at MOl of 0.1:1, 1:1 and 10:1 did not express a detectable level of inducible nitric oxide synthase (iNOS). Moreover, the B. pseudomallei infected cells released TNF-a only when they were infected with high MOl (10:1). Unlike the cells infected with B. pseudomallei, the cells infected with E. coli, and S. typhi expressed iNOS even at MOl of 0.1:1. These infected cells also released a significantly higher level of TNF-a at the low MOl ratio. The cells that were preactivated with IFN-oy prior to being infected with B. pseudomallei exhibited an enhanced production of iNOS and TNF-a release. The increased macrophage activation activity in the presence of IFN -oy also correlated with the restriction of the intracellular bacteria survival. Moreover, IFN-oy also prevented cell fusion and multinucleated cell formation induced by B. pseudomallei, a phenomenon recently described by our group. Altogether, these results indicate that internalization of B. pseudomallei failed to trigger substantial macrophage activation, a phenomenon which could prolong their survival inside the phagocytic cells and facilitate a direct cell to cell spreading of B. pseudomallei to neighboring cells.

show abstract

Extraction and characterization of lipopolysaccharide from Pseudomonas pseudomallei

Cited by 22 publications

References 5 publications

Fate of a Burkholderia pseudomallei Lipopolysaccharide Mutant in the Mouse Macrophage Cell Line RAW 264.7: Possible Role for the O-Antigenic Polysaccharide Moiety of Lipopolysaccharide in Internalization and Intracellular Survival

Fate of a Burkholderia pseudomallei Lipopolysaccharide Mutant in the Mouse Macrophage Cell Line RAW 264.7: Possible Role for the O-Antigenic Polysaccharide Moiety of Lipopolysaccharide in Internalization and Intracellular Survival

Involvement of Beta Interferon in Enhancing Inducible Nitric Oxide Synthase Production and Antimicrobial Activity of Burkholderia pseudomallei- Infected Macrophages

Burkholderia pseudomallei Interferes with Inducible Nitric Oxide Synthase (iNOS) Production: A Possible Mechanism of Evading Macrophage Killing

Contact Info

Product

Resources

About