Extraction and characterization of a natural rubber from <i>Euphorbia characias</i> latex

Spanò, Delia; Pintus, Francesca; Mascia, C; Scorciapino, Mariano Andrea; Casu, Mariano; Floris, Giovanni; Medda, Rosaria

doi:10.1002/bip.22044

Cited by 28 publications

(26 citation statements)

References 34 publications

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“…E. characias latex has been studied with regard to its antioxidant and non-protein components ( Pintus et al, 2013 ) but several proteins have been isolated and deeply characterized ( Mura et al, 2008 ; Pintus et al, 2009 ; Pintus et al, 2011 ; Dainese et al, 2014 ; Spanò et al, in press ). It has also been identified in E. characias , a cis -prenyl transferase ( Spanò et al, 2015 ) which is the enzyme responsible of the synthesis of the natural rubber occurring in the plant latex ( Spanò et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Antityrosinase activity ofEuphorbia characiasextracts

Pintus

Spanò

Corona

et al. 2015

PeerJ

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Tyrosinase is a well-known key enzyme in melanin biosynthesis and its inhibitors have become increasingly important because of their potential use as hypopigmenting agents. In the present study, the anti-melanogenic effect of aqueous and ethanolic extracts from Euphorbia characias leaves, stems, and flowers in cell-free and cellular systems was examined. All the extracts showed inhibitory effects against mushroom tyrosinase with leaf extracts exhibiting the lowest IC50 values of 24 and 97 µg/mL for aqueous and ethanolic extracts respectively. Enzyme kinetic analysis indicated that leaf aqueous extract acts as a mixed type inhibitor, while ethanolic extract shows a competitive inhibition effect on mushroom tyrosinase using L-DOPA as substrate. In addition, the inhibitory effect of leaf extracts on tyrosinase activity and melanin production was examined in murine melanoma B16F10 cells. Cellular tyrosinase activity as well as levels of melanin synthesis are reduced in a dose-dependent manner by extracts in cells treated with α-melanocyte stimulating hormone (α-MSH). The effects are comparable, and sometimes even better, than that of kojic acid, a well known tyrosinase inhibitor used for reference. All these results suggest that E. characias could be a great source of the natural inhibitors from tyrosinase and has the potential to be used as a whitening agent in therapeutic fields.

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Section: Introductionmentioning

confidence: 99%

Antityrosinase activity ofEuphorbia characiasextracts

Pintus

Spanò

Corona

et al. 2015

PeerJ

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“…В недавнем исследовании (Rzymski, 2014) для выделения каучука из кустарника гваюлы (Parthenium argentatum L.) использовали ацетон (полярный растворитель), в котором каучук не растворяется, ацетонитрил (среднеполярный растворитель) и циклогексан (неполярный растворитель), в которых каучук растворяется. Похожий подход был использован также для выделения каучука из молочая Euphorbia characias L. (Spano et al, 2012). Учитывая общую схожесть химических составов клеток растений, можно предположить, что эти методы подойдут и для кок-сагыза, к тому же в гваюле каучук, как и часть каучука в кок-сагызе, накапливается в паренхиме.…”

Section: технологии экстракции каучука из кок-сагыза для промышленностиunclassified

Russian dandelion (<i>Taraxacum kok-saghyz</i> Rodin): rubber extraction methods and prospects for biotechnological methods application

Kuluev

Minchenkov²,

Gumerova

2019

Biotehnologiâ i selekciâ rastenij

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Natural rubber is a strategic natural raw material, which is used in the production of tires and military equipment, in medicine and other industries. An alternative to Hevea brasiliensis (Willd. ex A. Juss.) Müll. Arg. and the most promising rubber plant is the Russian dandelion (Taraxacum kok-saghyz Rodin). The rubber that accumulates in the roots of this dandelion is not inferior in quality to the natural rubber from H. brasiliensis, and its content reaches 27% of the dry weight of roots. The purpose of this paper is to describe the economically important components of T. kok-saghyz roots, the main methods for extracting natural rubber from the roots, as well as the approaches to micropropagation and genetic transformation of kok-saghyz and related species. In the middle of the 20th century, the industrial method of isolating rubber from Russian dandelion in the USSR was based on preliminary treatment of the roots with a 2% solution of alkali, which could negatively affect rubber quality. Therefore, it is important to develop new, rapid, but at the same time, inexpensive methods of rubber extraction from T. koksaghyz. Some of them are considered in this paper. The breeding of Russian dandelion should be aimed at both increasing the root size and the content of rubber. In this regard, the development of laboratory express methods for rubber extraction is also important. The authors have developed and optimized a method for extracting rubber from dry plant tissue using polar solvents (water and acetone), with the final extraction with a non‑polar solvent (hexane). The developed rubber extraction protocol showed results comparable to the literature data. In order to create more productive plant forms, experiments are also being conducted on T. kok-saghyz micropropagation and genetic transformation. However, the number of such works is still very small, probably due to the low regenerative abilities of this dandelion species.

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“…In addition, several papers report on the presence, in E. characias latex, of proteins and enzymatic activities (Barbieri et al, 1983;Palocci et al, 2003;Pintus et al, 2010;Medda et al, 2011). Moreover, antioxidants, acetylcholinesterase inhibitory activities (Pintus et al, 2013), and a natural rubber (Span o et al, 2012) have been well identified and characterized.…”

Section: Introductionmentioning

confidence: 96%