Extraction and Assembly of Tissue-Derived Gels for Cell Culture and Tissue Engineering

Uriel, Shiri; Labay, Edwardine; Francis-Sedlak, Megan; Moya, Monica L.; Weichselbaum, Ralph R.; Ervin, Natalia; Cankova, Zdravka; Brey, Eric M.

doi:10.1089/ten.tec.2008.0309

Cited by 108 publications

(98 citation statements)

References 53 publications

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“…This complex combination not only guides cellular attachment but provides survival, migration, proliferation and differentiation cues (35)(36)(37)(38)(39)(40) associated with significant changes in cell behaviour. (41,42) The gel has been shown to retain its gylcosaminoglycan (GAG) content and to allow migration of fibroblasts in vitro.…”

Section: Acellular Materials Based Scaffoldsmentioning

confidence: 99%

Biomaterial‐Enhanced Cell and Drug Delivery: Lessons Learned in the Cardiac Field and Future Perspectives

et al. 2016

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Heart failure is a significant clinical issue. It is the cause of enormous healthcare costs worldwide and results in significant morbidity and mortality. Cardiac regenerative therapy has progressed considerably from clinical and preclinical studies delivering simple suspensions of cells, macromolecule, and small molecules to more advanced delivery methods utilizing biomaterial scaffolds as depots for localized targeted delivery to the damaged and ischemic myocardium. Here, regenerative strategies for cardiac tissue engineering with a focus on advanced delivery strategies and the use of multimodal therapeutic strategies are reviewed.

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Section: Acellular Materials Based Scaffoldsmentioning

confidence: 99%

Biomaterial‐Enhanced Cell and Drug Delivery: Lessons Learned in the Cardiac Field and Future Perspectives

et al. 2016

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“…We have recently developed a novel method for generating ECM-rich, tissue-derived hydrogels with chemical and physical properties that vary depending on the tissue source. [5,6] Hydrogels developed from this technique have shown promise in tissue engineering applications due to their ability to stimulate vessel assembly in vitro and vascularized tissue formation in vivo. [5][6][7] Hydrogels assembled from dermal tissue contain BM proteins, including laminin β3, collagen IV, and collagen VII, which are essential for proper skin function.…”

Section: What This Study Adds To the Fieldmentioning

confidence: 99%

“…[5,6] Hydrogels developed from this technique have shown promise in tissue engineering applications due to their ability to stimulate vessel assembly in vitro and vascularized tissue formation in vivo. [5][6][7] Hydrogels assembled from dermal tissue contain BM proteins, including laminin β3, collagen IV, and collagen VII, which are essential for proper skin function. [5,6] Dermis-based hydrogels may provide benefit for applications in wound healing or tissue engineering of skin.…”

Section: What This Study Adds To the Fieldmentioning

confidence: 99%

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Investigation of Dermis-derived hydrogels for wound healing applications

Engel¹,

Kao²,

Larson³

et al. 2015

Biomed J

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ell interactions with the extracellular matrices (ECM) in general and specifically with the dermal basement membrane (BM) play an important role in normal skin physiology and wound healing.[1] The BM is a sheet of fibrous extracellular material upon which the basal surfaces of epithelial cells rest. The degradation of existing matrix and synthesis of new BM is a limiting step in wound closure. [2] For this reason, ECM-based natural and synthetic materials have been investigated for applications in the regeneration or reconstruction of skin. [3,4] However, the materials investigated often feature ECM properties derived from areas other than skin. Materials that contain ECM features Background: Wound healing and skin tissue engineering are mediated, in part, by interactions between cells and the extracellular matrix (ECM). A subset of the ECM, basement membranes (BM), plays a vital role in regulating proper skin healing and function. Methods:ECM-rich, tissue-specific hydrogels were extracted and assembled from dermis samples. These hydrogels contain BM proteins vital to skin regeneration, including laminin β3, collagen IV, and collagen VII. The extracts could be assembled to form hydrogels by either temperature or pH mechanism, with the mechanical properties and structure varying with the mechanism of assembly. A wound healing model was developed to investigate the ability of these hydrogels to enhance healing with a single application in vivo. Results:The pH, but not temperature gels were easily applied to the wounds. There were no signs of increased inflammation due to the application of the hydrogels. The width of granulation tissue at the first week was reduced (p = 0.064) relative to controls with the application of hydrogel. There were no changes in wound closure rates or vessel density. Conclusions: Dermis-derived hydrogels contain BM proteins important for skin regeneration. They can be easily applied, but their poor mechanical strength and rapid degradation may hinder their biological effects. (Biomed J 2015;38:58-64)

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“…[11,12] Applying this technique, they derived hydrogels from dermal extracts of rat skin and determined the functional properties of the resultant hydrogels by atomic force electroscopy. Satisfied with the results, the team then applied the hydrogels to the surface of wounds made to the back skin of rats and compared wound healing over the course of several weeks using rats receiving no treatment as a control.…”

Section: Spotlight On Original Articlescan Skin-based Hydrogels Help mentioning

confidence: 99%

Tudors, tolerance, and pregnancy loss

Walton¹

2015

Biomed J

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Staff Writer at the Biomedical Journal, Dronningens gate 56, Trondheim, NorwayRecurrent pregnancy loss is an unfortunate fact of life for many women. This special issue of the Biomedical Journal includes four review articles from renowned experts discussing the factors important for a successful pregnancy and, in particular, the complex immunological dialog between mother and fetus. We also focus on an animal study investigating the utility of skin-derived hydrogels for wound healing, unearth the details of a rare stomach cancer, and discover new applications for silver in dentistry. (Biomed J 2015;38:1-4)

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Extraction and Assembly of Tissue-Derived Gels for Cell Culture and Tissue Engineering

Cited by 108 publications

References 53 publications

Biomaterial‐Enhanced Cell and Drug Delivery: Lessons Learned in the Cardiac Field and Future Perspectives

Biomaterial‐Enhanced Cell and Drug Delivery: Lessons Learned in the Cardiac Field and Future Perspectives

Investigation of Dermis-derived hydrogels for wound healing applications

Tudors, tolerance, and pregnancy loss

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