Extraction and Antioxidant Activity of Flavones from Salvia miltiorrhiza

Abstract

1. Objective: Salvia miltiorrhiza is a traditional Chinese herb medicine and is widely used in clinic application. This study is intended to extract flavones from Salvia miltiorrhiza by ultrasonic method in order to evaluate its antioxidation activity. 2. Methods: (1) Spectrophotometry is used to determine content of flavones from Salvia miltiorrhiza; (2) Ultrasonic method was used to extract flavones from Salvia miltiorrhiza and 80% ethanol-water as the solution. The effect of the different volume of solvent, extraction time and frequency were conducted (3) The antioxidation of Salvia miltiorrhiza flavones is evaluated by pyrogallol autoxidation and deoxyribose degradation. 3. Results: (1) Phenol -sulfuric acid method was established to test the content of flavones from Salvia miltiorrhiza. The results were in good linear relationship, and y = 0.0119 x -0.0061, R 2 = 0.9996. (2) The extraction process of Salvia miltiorrhiza flavones by ultrasonic method was 5g raw material, 245mL 80% ethanol-water solution, twice extraction, 40 min for each. (3) Antioxidtion tests indicated that with the concentration 0.05mg/mL of flavones, the inhibition rate for superoxide anion radical could reach 76.89% and 58.54% for hydroxyl radical, respectively. And the reducing power was likely to increase with the increasing of flavones concentration. 4. Conclusion: Ultrasonic method could effectively extract flavones from Salvia miltiorrhiza. Antioxidation tests indicate that flavones from Salvia miltiorrhiza have a certain removal effect on superoxide anion radical and hydroxyl radical.