Extracting Water-Soluble Proteins from the Red Macroalgae <i>Gracilaria</i> sp. with Pulsed Electric Field in a Continuous Process

Kashyap, Mrinal; Ghosh, Supratim; Steinbruch, Efraim; Levkov, Klimentiy; Israel, Álvaro; Kiran, Boggavarapu; Golberg, Alexander

doi:10.1021/acsfoodscitech.2c00247

Cited by 6 publications

(2 citation statements)

References 70 publications

(119 reference statements)

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“…We recently reported the use of a continuous PEF device to extract watersoluble proteins from Gracilaria sp. biomass (Kashyap et al 2022). The current study aims to further enhance protein extraction by combining PEF with pH shift.…”

Section: )mentioning

confidence: 99%

“…PH shift is a promising technology for seaweed protein extraction, used previously on Porphyra umbilicalis, Ulva lactuca, and Saccharina latissimi (Harrysson et al 2018), and some other species like Ulva fenestrate (Juul et pretreatment methods except our previous study on the Gracilaria sp. using a continuous PEF device (Kashyap et al 2022). The present study represents the rst report of combining PEF and pH shift to improve total solute and protein release from Gracilaria sp.…”

Section: )mentioning

confidence: 99%

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Protein and solute release from the red seaweed, Gracilaria sp., using a high voltage pulsed electric field and pH shift

Kashyap,

Ghosh,

Levkov

et al. 2024

Preprint

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This study explores the potential of Gracilaria sp., a red seaweed species, as a source of protein ingredients. We investigated the effects of high voltage pulsed electric field (PEF) pre-treatment, followed by either water-mediated extraction or pH shift, on the total-solute (“dry matter”) and protein release, and the content of essential and branched-chain amino acids (BCAA) in the extracts. The PEF was administered using a custom-made generator within a specially designed electroporation cell with sliding electrodes. The treatment parameters comprised 1000 V, a 50 µs pulse duration, 200 pulses at a 3 Hz frequency, and an inter-electrode gap ranging from 3.9 to 4.4 mm. The pH shift extraction was executed through combination of fractions extracted sequentially at pH-shift steps spanning the pH range of 1 to 12. Both PEF and pH played pivotal roles in the fractionation of Gracilariasp., enhancing protein release compared to water-mediated extraction, PEF pre-treatment, or pH shift alone. The combination of PEF and sequential pH washes (pH 1 to 12) resulted in a total solute release of 9.61±0.04% and protein release of 28.02±0.2% with essential amino acid (EAA) content of 63.6±5.3%, and BCAA content of 3.47±0.06%. It is noteworthy that PEF significantly increased the EAA and BCAA content of the protein extracts, irrespective of pH shifts. These findings underscore the promising potential of PEF pre-treatment in producing functional food ingredients derived from macroalgal sources.

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Section: )mentioning

confidence: 99%

Section: )mentioning

confidence: 99%

Protein and solute release from the red seaweed, Gracilaria sp., using a high voltage pulsed electric field and pH shift

Kashyap,

Ghosh,

Levkov

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

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High voltage pulsed electric field and electroporation technologies for algal biomass processing

Kashyap,

Ghosh,

Bala

et al. 2023

J Appl Phycol

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High-Voltage Pulsed Electric Fields and pH Shift Process for Protein and Solute Release from Gracilaria sp., Red Edible Seaweed

Kashyap,

Ghosh,

Levkov

et al. 2024

Food Bioprocess Technol

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This study proposes a process based on the application of high-voltage pulsed electric field (PEF) in combination with pH shift for protein and solute release from fresh edible seaweed from Gracilaria sp.. The PEF was administered using a custom-made generator within and electroporation cell with sliding electrodes. The applied PEF parameters comprised 1000 V applied voltage, 50 µs pulse duration, 200 pulses delivered at 3 Hz frequency, and an inter-electrode gap ranging from 3.9 to 4.4 mm. The pH shift extraction was performed through a combination of fractions extracted sequentially at pH-shift steps spanning the pH range of 1 to 12. Both PEF and pH played pivotal roles in the fractionation of Gracilaria sp., enhancing protein release compared to water extraction, PEF pre-treatment, or pH shift alone. The combination of PEF and sequential pH washes (pH 1 to 12) resulted in a total solute release of 9.61 ± 0.04% and protein release of 28.02 ± 0.2% with essential amino acid (EAA) content of 63.6 ± 5.3%, and branched amino acids (BCAA) content of 3.47 ± 0.06%. It is noteworthy that PEF significantly increased the EAA and BCAA content of the protein extracts, irrespective of pH shifts. These findings underscore the promising potential of PEF pre-treatment in extracting proteins from edible seaweeds.

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Extracting Water-Soluble Proteins from the Red Macroalgae Gracilaria sp. with Pulsed Electric Field in a Continuous Process

Cited by 6 publications

References 70 publications

Protein and solute release from the red seaweed, Gracilaria sp., using a high voltage pulsed electric field and pH shift

Protein and solute release from the red seaweed, Gracilaria sp., using a high voltage pulsed electric field and pH shift

High voltage pulsed electric field and electroporation technologies for algal biomass processing

High-Voltage Pulsed Electric Fields and pH Shift Process for Protein and Solute Release from Gracilaria sp., Red Edible Seaweed

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