1994
DOI: 10.1007/978-1-59259-513-6_14
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Extracting Thermodynamic Data From Equilibrium Melting Curves for Oligonucleotide Order-Disorder Transitions

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Cited by 94 publications
(86 citation statements)
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“…The optical and calorimetric melting profiles were analyzed as previously described 36,37 to extract melting temperatures, T,,, and T,,, (from uv and DSC) as well as direct, model-independent transition enthalpies, AH,,, (from DSC), and model-dependent, van't Hoff enthalpies, AHvH (from uv and DSC). We also have combined the calorimetrically determined AH value with the optically derived concentration dependence of T,,, data to evaluate the effective molecularity of the duplex melting process, as well as to evaluate the free energy change, AG, associated with duplex disruption.…”
Section: Duplex Meltingmentioning
confidence: 99%
“…The optical and calorimetric melting profiles were analyzed as previously described 36,37 to extract melting temperatures, T,,, and T,,, (from uv and DSC) as well as direct, model-independent transition enthalpies, AH,,, (from DSC), and model-dependent, van't Hoff enthalpies, AHvH (from uv and DSC). We also have combined the calorimetrically determined AH value with the optically derived concentration dependence of T,,, data to evaluate the effective molecularity of the duplex melting process, as well as to evaluate the free energy change, AG, associated with duplex disruption.…”
Section: Duplex Meltingmentioning
confidence: 99%
“…The effects of these mutations on the stability of the TAR RNA-aptamer complex were analyzed by thermal denaturation monitored by UV-absorption spectroscopy (26,27). The binding kinetics were determined by using surface plasmon resonance (SPR).…”
mentioning
confidence: 99%
“…6 The transitions are accompanied by large energy change ( 8-10 kcal/mol for the sequences being discussed) and a clear melting point, characteristic of single-stranded self-transition for hairpin formation, as opposed to loose interstrand aggregation or self-helix formation which exhibit small energy variation and broad melting. 26 Overall it is observed that of the two families, the (GACX) motif can direct the formation of structures stabilized under acidic conditions provided that the CG base stack is absent from the repeat motif. These structures have the potential to differ from classical W-C DNA duplexes, due to the abundance of non-canonical pairings between the protonated bases.…”
Section: Structural Properties Of Dna Oligomers 163mentioning
confidence: 96%
“…24 The Marquardt nonlinear least squares method, implemented within the program was used to fit the absorbance versus temperature curves. The melting temperature, T m , was also calculated using a two-state analysis method for non self-complementary associations, 25,26 (See Supporting Information).…”
Section: Determination Of Thermodynamic Parameters Using Meltwin 35mentioning
confidence: 99%