Extractable latex allergens in airborne glove powder and in cut glove pieces

Kujala, V.; Alenius, Harri; Palosuo, Timo; Karvonen, J.; Pfäffli, Pirkko; Reijula, Kari

doi:10.1046/j.1365-2222.2002.01413.x

Cited by 23 publications

(15 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For the first experiment, protein extracts were prepared according to the methods of Kujala et al (2002) and Bjorksten et al (1980), with or without the inclusion of a mix of protease inhibitors as described in the second protocol. Analysis of these extracts by IgE-immunoblot shows a high level of staining in the low-and highmolecular weight region when no protease inhibitors were included.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Comparison and Functional Evaluation of the Allergenicity of Different Hazelnut (Corylus avellana) Protein Extracts

et al. 2010

View full text Add to dashboard Cite

This paper investigates two methodological issues of hazelnut protein extraction: the use of protease inhibitors during protein extraction and the effect of defatting hazelnuts before protein extraction. Different protein extracts from hazelnuts were analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and IgE-immunoblotting to evaluate the presence of allergens in the extract. The allergy-provoking potential of these extracts was assessed by the Basophil Activation Test. This functional test relies on in vitro stimulation of human basophils from hazelnut-allergic patients with the different extracts. SDS-PAGE and IgE-immunoblot analysis of hazelnut extracts prepared without protease inhibitors revealed partial degradation of the proteins in the extracts. This observation was confirmed by the Basophil Activation Test, as the percentage of activated basophils was lower as compared to extracts prepared with protease inhibitors. Biochemical and functional evaluation of a defatted hazelnut extract did not reveal differences in comparison with non-defatted extracts. This study is the first to report on the use of the Basophil Activation Test applying human basophils for functional evaluation of protein extracts from hazelnuts. The results demonstrate that the use of protease inhibitors during extraction is essential for the stability and functionality of a protein extract, whereas defatting does not affect the quality of the protein extract.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Hazelnut proteins were extracted using the methods according to Kujala et al (2002) and Bjorksten et al (1980), with (+) or without (−) the inclusion of a mix of protease inhibitors, respectively. The two extraction methods are called A and B, respectively.…”

Section: Protein Extractionmentioning

confidence: 99%

Comparison and Functional Evaluation of the Allergenicity of Different Hazelnut (Corylus avellana) Protein Extracts

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Instead, they were sensitized to rHev b 5 and/or rHev b 6.01. This could be explained by the physical properties of the allergens: Hev b 6.01 attaches more easily to powder to become aerosolized (16), and is able to sensitize through the inhalatory route; in contrast, Hev b 1 takes much harder to aerosolize (16), and requires direct contact of latex with the blood or mucosas, in operations, to sensitize. IgE to rHev b 3 appeared only in children with SB, and IgE to rHev b 1 in 15 of 20 of these children, while it was found in only two of six non‐SB multioperated children.…”

Section: Discussionmentioning

confidence: 99%

Patterns of latex allergen recognition in children sensitized to natural rubber latex

Pamies¹,

Oliver²,

Raulf‐Heimsoth³

et al. 2005

Pediatric Allergy Immunology

View full text Add to dashboard Cite

Single recombinant latex allergens permit the study of the pattern of sensitization to individual allergens. We aimed to quantify the IgE-response to individual latex allergens in children sensitized to latex. The study group included 31 latex-sensitized children: 26 operated at least twice, 20 of them with spina bifida; two children with one operation and three atopic non-operated children. IgE antibodies to rHev b 1, rHev b 3, rHev b 5, rHev b 6.01, rHev b 7.02 and rHev b 8, coupled to ImmunoCAPs, were measured in each serum. IgE responses to rHev b 1, rHev b 5 and rHev b 6.01 were found in 17 children each, and their mean +/- s.d. levels were 5 +/- 7.4, 16.8 +/- 14 and 10 +/- 18 kU/l, respectively. IgE responses to rHev b 3 (4 +/- 5.4 kU/l) were found in eight children. Two children had IgE to rHev b 7 (1.7 and 3.2 kU/l), and none to rHev b 8. Four sera were negative to all tested recombinant allergens. We divided the patients in three groups: sensitized only to rHev b 1, sensitized only to rHev b 5 and/or rHev b 6.01, and sensitized to both rHev b 1 and to rHev b 5 and/or rHev b 6.01. The three groups had the same profile of clinical features. Hev b 5 induces the quantitatively higher IgE responses in children with multiple surgeries sensitized to latex. Responses to Hev b 6.01 equal those of Hev b 1.

show abstract

“…This results in an unstable, uncharacterized product of variable potency. The lack of characterization of the hevea allergen in such products has resulted in false‐negative results due to low allergen content or systemic reaction due to high levels of hevea allergen 3,59–61…”

Section: Diagnosismentioning

confidence: 99%

Latex Allergy

Gawchik

2011

Mount Sinai J Medicine

View full text Add to dashboard Cite

Allergy to natural rubber latex is an important clinical condition that occurred after the institution of universal precautions to protect healthcare workers. A rapid increase and production of both examination and surgical gloves resulted in an epidemic of allergy to latex protein. Healthcare workers in both the medical and dental environments, as well as specific groups of individuals including those with spina bifida, myelodysplasia, and food allergies (banana, kiwi, avocado, and others), were at increased risk of sensitization. Clinical symptoms in the latex allergic individual ranged from type I hypersensitivity reaction including rhinoconjunctivitis, asthma, and systemic reaction to type IV hypersensitivity reaction, which occur from the chemicals added during the manufacturing process. Diagnosis of latex allergy is based on a clinical history that correlates the development of symptoms in relationship to exposure. In the United States there are no skin tests approved by the Food and Drug Administration. Therefore a combination of clinical judgment and serologic testing such as ImmunoCAP and Immulite is helpful. The primary treatment of latex allergy is avoidance of exposure to the latex protein.

show abstract

Extractable latex allergens in airborne glove powder and in cut glove pieces

Abstract: An elevated NRL aeroallergen level is rather related to a high level of airborne glove powder than to a high concentration of extractable NRL allergen in medical gloves.

Cited by 23 publications

References 24 publications

Comparison and Functional Evaluation of the Allergenicity of Different Hazelnut (Corylus avellana) Protein Extracts

Comparison and Functional Evaluation of the Allergenicity of Different Hazelnut (Corylus avellana) Protein Extracts

Patterns of latex allergen recognition in children sensitized to natural rubber latex

Latex Allergy

Contact Info

Product

Resources

About