Extractability and chromatographic separation of rice endosperm proteins

Borght, Anne Van Der; Vandeputte, GE; Derycke, Veerle; Brijs, Kristof; Daenen, Geert; Delcour, Jan A.

doi:10.1016/j.jcs.2006.03.005

Cited by 75 publications

(36 citation statements)

References 20 publications

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“…Van Der Borght et al (2006) found prolamin between 16 and 10 kDa, which were similar to the values observed in 12 O. glumaepatula genotypes. Chandi & Sogi (2007) found slightly larger prolamin, with molecular masses of 28-24 kDa.…”

Section: Resultssupporting

confidence: 87%

Storage protein profile and amino acid content in wild rice Oryza glumaepatula

Santos

Silveira

Martin-Didonet

et al. 2013

Pesq. agropec. bras.

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-The objective of this work was to determine the total protein profile and the contents of the four major protein fractions (albumin, globulin, prolamin and glutelin) and of the amino acids in the endosperm of the rice wild species Oryza glumaepatula. The experiment was performed with 29 accessions of this species, collected from 13 Brazilian locations, and two commercial cultivars. Protein samples were prepared using dried, polished, and ground grains to obtain homogeneous, dry flour used in the preparation of extracts. Oryza glumaepatula accessions were identified with the highest levels of total protein, albumin and glutelin protein fractions, and amino acids (with the exception of tryptophan) in comparison to the two analized rice cultivars. The albumin and glutelin profiles in SDS-Page were distinct between rice cultivars and O. glumaepatula. This wild species has the potential to increase the nutritional quality of rice storage protein through interspecific crosses.Index terms: Oryza sativa, genetic resources, nutritional quality, plant breeding, SDS-Page. Perfil da proteína de reserva e conteúdo de aminoácidos no arroz silvestre Oryza glumaepatulaResumo -O objetivo deste trabalho foi determinar os perfis de proteína total e o conteúdo das quatro principais frações proteicas (albumina, globulina, prolamina e glutelina) e de aminoácidos no endosperma da espécie de arroz silvestre Oryza glumaepatula. O experimento foi realizado com 29 acessos dessa espécie, coletados em 13 locais no Brasil, e duas cultivares comerciais. Amostras de proteínas foram preparadas com grãos secos, polidos e moídos, para obtenção de uma farinha seca e homogênea, usada no preparo dos estratos. Acessos de O. glumaepatula foram identificados com os maiores níveis de proteína total, frações proteicas de albumina e glutelina, e aminoácidos (com exceção do triptofano), em comparação às duas cultivares de arroz avaliadas. Os perfis de albumina e glutelina em SDS-PAGE foram distintos entre as cultivares de arroz e O. glumaepatula. Essa espécie silvestre tem o potencial de aumentar a qualidade nutricional da proteína de reserva do arroz por meio de cruzamentos interespecíficos.Termos para indexação: Oryza sativa, recursos genéticos, qualidade nutricional, melhoramento de plantas, SDS-Page.

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Section: Resultssupporting

confidence: 87%

Storage protein profile and amino acid content in wild rice Oryza glumaepatula

Santos

Silveira

Martin-Didonet

et al. 2013

Pesq. agropec. bras.

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“…As mentioned earlier, it is assumed that 100% of the proteins were extracted from the native rice flour with 2.0% SDS/1.5% DTT/ 6.0 M urea (Van Der Borght et al, 2006). The level of extractable protein is calculated from the peak areas under the complete protein profile and expressed as percentage of the native flour extracted with 2.0% SDS/1.5% DTT/6.0 M urea (similar to Van Der Borght et al, 2006).…”

Section: Resultsmentioning

confidence: 99%

“…Solubilised proteins were recovered by centrifugation (2800 g, 15 min, ambient temperature), filtered (0.45 mm PP filter) and further injected on the separative system. These experimental conditions were chosen in order to achieve maximum extractability (Van Der Borght et al, 2006).…”

Section: Determination Of the Protein Molecular Weight Profile By Higmentioning

confidence: 99%

Impact of extrusion parameters on the properties of rice products: A physicochemical and X-ray tomography study

et al. 2015

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“…Rwandan cultivars exhibited a high proportion of low molecular weight proteins which is attributable to the evaluation of whole rice grain in this study. According to Van der Borght et al [65], the fast eluting fraction contains α-and β-glutelin subunits, which are contributing to the mixing properties of the rice flour.…”

Section: 2mentioning

confidence: 99%

Quality and Grain Yield Attributes of Rwandan Rice (Oryza sativaL.) Cultivars Grown in a Biotron Applying Two NPK Levels

Mukamuhirwa

Hovmalm

Ortíz

et al. 2018

Journal of Food Quality

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High-yielding rice cultivars with good processing quality and rich in nutrition suitable to a changing climate are of particular importance for future rice-based food production. Here, seven Rwandan rice cultivars were grown in a climate chamber of the biotron facility at the Swedish University of Agricultural Sciences, to be evaluated for their grain yield, nutritional composition, and dough mixing properties. Two di erent levels of inorganic fertilizer were applied weekly from the seedling stage until owering. Signi cant di erences for grain yield and quality attributes were found between cultivars. Jyambere showed signi cantly the highest yield while Ingwizabukungu, Nemeyubutaka, and Jyambere were high in mineral elements content. Ndamirabahinzi and Mpembuke had the highest levels of TPC and TAC. Generally, the lower fertilizer dose resulted in a better performance of the cultivars for both yield and quality attributes. Signi cantly higher content of Fe, Ca, and Ba was found in grains from the moderate fertilizer dose, whereas K, Na, P, S, Zn, Cd, and Pb increased in grains from the higher fertilizer dose. e cultivar Ndamirabahinzi showed less variability of evaluated characters across fertilizer doses. e results from this study may be used for rice breeding of cultivars with high yield and good grain quality.

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Extractability and chromatographic separation of rice endosperm proteins

Cited by 75 publications

References 20 publications

Storage protein profile and amino acid content in wild rice Oryza glumaepatula

Storage protein profile and amino acid content in wild rice Oryza glumaepatula

Impact of extrusion parameters on the properties of rice products: A physicochemical and X-ray tomography study

Quality and Grain Yield Attributes of Rwandan Rice (Oryza sativaL.) Cultivars Grown in a Biotron Applying Two NPK Levels

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