Extract of cactus (Opuntia ficus indica) cladodes scavenges reactive oxygen species in vitro and enhances plasma antioxidant capacity in humans

Ávila-Nava, Azalia; Calderón‐Oliver, Mariel; Medina-Campos, Omar Noel; Zou, Tao; Gu, Liwei; Torres, Nimbe; Tovar, Armando R.; Pedraza‐Chaverrí, José

doi:10.1016/j.jff.2014.05.009

Cited by 55 publications

(32 citation statements)

References 59 publications

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“…Several studies have shown improved antioxidant capacity of plasma after dietary supplementation [57][58][59][60] and especially in this study [61], the addition of catechins or tea extract to human plasma and tea consumption by humans were also reported to increase the total antioxidant capacity of plasma. According to Adefolaju et al it was shown that a diets supplemented with…”

Section: Plasma Antioxidant Capacitysupporting

confidence: 53%

Antioxidant Activity Assessment of Tamus Communis L. Roots

Zerargui

Baghiani

Khennouf

et al. 2016

Int J Pharm Pharm Sci

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Objective: The aim of this study was to evaluate the antioxidant activities of Tamus communis L. (T. communis) roots. This plant is used in folk medicine in the treatment of rheumatism, arthritis and lumbago. Methods:The extraction of polyphenols has been followed by the determination of total polyphenols and flavonoids. The methanol extract (MeOHE) was chromatographied on a silica gel column. The column was eluted with chloroform and then with chloroform/methanol mixtures of increasing polarity, 6 main fractions (FI--FVI)) were collected and grouped according to their TLC (Thin layer chromatography). Several methods were used to evaluate the in vitro antioxidant activity such as DPPH (2, 2'-diphenyl-1-picrylhydrazy), bleaching of β-carotene for 24 h, lipid peroxidation and anti-hemolytic activity. An in vivo approach was carried out on mice treated with CE (crud extract) at a dose of 100 mg/Kg/day for 21 consecutive days, and one group was treated with vitamin C (Vit C 50 mg/kg) as a standard drug. To determine the improvement of antioxidant potential, basic biochemical parameters were used in tissue (liver), plasma and whole blood. Results:The results showed that the highest level of polyphenols was recorded in FII (73.143±0.009 mg GA-Eq/g extract (Gallic Acid Equivalent))/g of extract) followed by MeOHE (69.786±0.10 mg GA-Eq/g extract). Total flavonoid contents of extracts/fractions showed that MeOHE contains the highest level. Inhibition of DPPH radical showed that MeOHE (0.1187±0.025 mg/ml) and CE (0.2363±0.019 mg/ml) have the most efficient antioxidant activity. All the extracts were capable of inhibiting the bleaching of β-carotene by scavenging linoleate derived free radicals, and all the studied extracts have a very important antihemolytic activity (p<0.001) and revealed an extension of hemolysis half time from 70.34±2.15 min of CTL (AAPH (2,2-azo-bis (2-amidinopropane) dihydrochloride)) and 207±5.65 min for FI. The in vivo assay was exhibited a significant decrease (54.09%) of the content of malondialdehyde (MDA) in the liver and increased glutathione (GSH) and catalase (47.30% and 46.87% respectively). Conclusion:These findings suggest that the extract obtained from T. communis have active substances contributing to the increase in natural antioxidant potential.

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Section: Plasma Antioxidant Capacitysupporting

confidence: 53%

Antioxidant Activity Assessment of Tamus Communis L. Roots

Zerargui

Baghiani

Khennouf

et al. 2016

Int J Pharm Pharm Sci

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“…Recently, many scientific papers have focused their attention on health promoting properties of several foods (Avila-Nava et al, 2014;Braithwaite et al, 2014;Tahergorabi, Matak, & Jaczynski, 2014). The role of functional foods and their components in disease risk reduction, treatment and promotion of human health has been demonstrated (Griffiths, Abernethy, Schuber, & Williams, 2009;Lobo, Patil, Phatak, & Chandra, 2010;Tenore, Novellino, & Basile, 2012;Vulíc et al, 2014;Zhuang, Tang, & Yuan, 2013).…”

Section: Introductionmentioning

confidence: 99%

Nutraceutical properties of phycocyanin

Fernández-Rojas

Hernández‐Juárez²,

Pedraza‐Chaverrí

2014

Journal of Functional Foods

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200

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“…Antioxidative flavonoids, quercetin and its derivatives were isolated from the stems of OFS [45]. Avila-Nava et al [46] reported the ROS scavenging activity of stem extract, and isolated several polyphenols such as quercetin, isorhamnetin and kaempferol. Several antioxidants have been reported to activate AMPK.…”

Section: Discussionmentioning

confidence: 99%

Hypoglycemic Effect of Opuntia ficus-indica var. saboten Is Due to Enhanced Peripheral Glucose Uptake through Activation of AMPK/p38 MAPK Pathway

et al. 2016

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Opuntia ficus-indica var. saboten (OFS) has been used in traditional medicine for centuries to treat several illnesses, including diabetes. However, detailed mechanisms underlying hypoglycemic effects remain unclear. In this study, the mechanism underlying the hypoglycemic activity of OFS was evaluated using in vitro and in vivo systems. OFS treatment inhibited α-glucosidase activity and intestinal glucose absorption assessed by Na+-dependent glucose uptake using brush border membrane vesicles. AMP-activated protein kinase (AMPK) is widely recognized as an important regulator of glucose transport in skeletal muscle, and p38 mitogen-activated protein kinase (MAPK) has been proposed to be a component of AMPK-mediated signaling. In the present study, OFS dose-dependently increased glucose uptake in L6 muscle cells. The AMPK and p38 MAPK phosphorylations were stimulated by OFS, and inhibitors of AMPK (compound C) and p38 MAPK (SB203580) abolished the effects of OFS. Furthermore, OFS increased glucose transporter 4 (GLUT4) translocation to the plasma membrane. OFS administration (1 g/kg and 2 g/kg body weight) in db/db mice dose-dependently ameliorated hyperglycemia, hyperinsulinemia, and glucose tolerance. Insulin resistance assessed by homeostasis model assessment of insulin resistance and quantitative insulin sensitivity check index were also dose-dependently improved with OFS treatment. OFS administration improved pancreatic function through increased β-cell mass in db/db mice. These findings suggest that OFS acts by inhibiting glucose absorption from the intestine and enhancing glucose uptake from insulin-sensitive muscle cells through the AMPK/p38 MAPK signaling pathway.

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Extract of cactus (Opuntia ficus indica) cladodes scavenges reactive oxygen species in vitro and enhances plasma antioxidant capacity in humans

Cited by 55 publications

References 59 publications

Antioxidant Activity Assessment of Tamus Communis L. Roots

Antioxidant Activity Assessment of Tamus Communis L. Roots

Nutraceutical properties of phycocyanin

Hypoglycemic Effect of Opuntia ficus-indica var. saboten Is Due to Enhanced Peripheral Glucose Uptake through Activation of AMPK/p38 MAPK Pathway

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