Extract from the fermented soybean product Natto inhibits Vibrio biofilm formation and reduces shrimp mortality from Vibrio harveyi infection

Yatip, Pattanan; Teja, D. Nitin Chandra; Flegel, Timothy W.; Soowannayan, Chumporn

doi:10.1016/j.fsi.2017.11.008

Cited by 30 publications

(19 citation statements)

References 33 publications

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“…However, in the current study, strain TOA5001 did not affect the mRNA levels of immune-related genes, suggesting that strain TOA5001 prevented AHPND by some means other than immune stimulation. Extracts from B. subtilis -fermented soybean were reported to inhibit Vibrio biofilm formation and to reduce the mortality of shrimp challenged by a pathogenic strain of Vibrio harveyi , but they had no bactericidal effect 46 . Similarly, the substance(s) produced by strain TOA5001 did not kill strain D6, but it did moderately inhibit its growth.…”

Section: Discussionmentioning

confidence: 99%

Analysis of microbiota in the stomach and midgut of two penaeid shrimps during probiotic feeding

Imaizumi

Tinwongger

Kondo

et al. 2021

Sci Rep

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In mammals, the intestine harbors numerous bacteria that play an important role in health. Intestinal microbiota have also been thought to be an important factor in the health of shrimp. However, the barrier systems of the digestive tracts of shrimp seem to be different from those of mammals. In this study, we analyzed the bacterial composition in the stomach and midgut of two species of shrimp during administration of a probiotic, Bacillus amyloliquefaciens strain TOA5001 by analysis of 16S rRNA genes with Illumina sequencing technology. Whiteleg shrimp Litopenaeus vannamei were observed under laboratory conditions and kuruma shrimp Marsupenaeus japonicus were observed in an aquaculture farm. The diversities of bacteria in the stomachs of both shrimps were significantly higher than those in the midgut. Also, the microbiota changed during probiotic feeding. Feeding whiteleg shrimp the probiotic after being challenged with an acute hepatopancreatic necrosis disease (AHPND)-causing strain of Vibrio parahaemolyticus increased their survival compared to the control group, which suggested that the probiotic prevented AHPND. These results appear to show that a probiotic can affect the microbiota throughout digestive tract of penaeid shrimps and that probiotic can have a role in preventing disease.

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Section: Discussionmentioning

confidence: 99%

Analysis of microbiota in the stomach and midgut of two penaeid shrimps during probiotic feeding

Imaizumi

Tinwongger

Kondo

et al. 2021

Sci Rep

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“…Biofilm formation could enhance the resistance to antibiotics (Khandekar et al., ; Yatip, Teja, Flegel, & Soowannayan, ) and host immune killing (Abdallah, Mahmoud, & Abdel‐Rahim, ; Chenia & Duma, ; Lange et al., ), while biofilm formation of Vibrio depends on complex regulatory processes (Cai et al., ). rpoS is involved in biofilm production in several bacteria, such as E. coli , Pseudomonas aeruginosa and Edwardsiella tarda (Xiao et al., ), although no such research about V. alginolyticus has yet been reported.…”

Section: Discussionmentioning

confidence: 99%

“…Biofilm formation could enhance the resistance to antibiotics (Khandekar et al, 2018;Yatip, Teja, Flegel, & Soowannayan, 2018) and rpoS is involved in biofilm production in several bacteria, such as E. coli, Pseudomonas aeruginosa and Edwardsiella tarda (Xiao et al, 2009), although no such research about V. alginolyticus has yet been reported. In the present study, results showed that the rpoS stably silenced strain can substantially reduce biofilm production compared with the wild-type strain.…”

Section: Discussionmentioning

confidence: 99%

The role of rpoS in the regulation of Vibrio alginolyticus virulence and the response to diverse stresses

Huang

Guo

et al. 2019

Journal of Fish Diseases

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Vibrio alginolyticus is a leading aquatic pathogen, causing huge losses to aquaculture. rpoS has been proven to play a variety of important roles in stress response and virulence in several bacteria. In our previous study, upon treatment with Cu2+, Pb2+, Hg2+ and low pH, the expression levels of rpoS were downregulated as assessed by RNA‐seq, while impaired adhesion ability was observed, indicating that rpoS might play roles in the regulation of adhesion. In the present study, the RNAi technology was used to knockdown rpoS in V. alginolyticus. In comparison with wild‐type V. alginolyticus, RNAi‐treated bacteria showed significantly impaired abilities of adhesion, growth, haemolytic, biofilm production, movement and virulence. Meanwhile, alterations of temperature, salinity, pH and starvation starkly affected rpoS expression. The present data suggested that rpoS is a critical regulator of virulence in V. alginolyticus; in addition, rpoS regulates bacterial adhesion in response to temperature, pH and nutrient content changes. These are helpful to explore its pathogenic mechanism and provide reference for disease control.

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“…In this study, a significantly higher survival rate following V. mimicus challenge was observed in marron fed FPBM than the marron offered the control diet. Similarly, fermented soybean product in the diet of white leg shrimp, Penaeus vannamei significantly increased the survival rate against Vibrio harveyi when compared with control shrimp given diet without these product (Yatip et al, 2018). However, mortality of freshwater prawn, Macrobrachium nipponense against Aeromonas hydrophila was not significantly different between the control and diets with up to 50% of fermented soybean meal although it increased significantly in 75 and 100% FSM treated groups (Ding et al, 2015).…”

Section: Discussionmentioning

confidence: 87%

Fermented Animal Source Protein as Substitution of Fishmeal on Intestinal Microbiota, Immune-Related Cytokines and Resistance to Vibrio mimicus in Freshwater Crayfish (Cherax cainii)

et al. 2020

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A feeding trial was carried out to evaluate the effects of substitution of fishmeal (FM) by dietary poultry by-product meal, fermented by Lactobacillus casei and Saccharomyces cerevisiae on growth, intestinal health, microbial composition, immune related cytokines and disease resistance of freshwater crayfish, marron (Cherax cainii) against Vibrio mimicus. Two isonitrogenous and isocaloric diets were formulated by replacing FM protein with fermented poultry by-product meal (FPBM) protein at 0% (Control) and 75% (FPBM), and fed marron for 70 days. The results indicated no significant difference (P > 0.05) in final body weights between two groups of marron, whilst intestinal microvilli number per fold was increased in marron fed FPBM than the control. The 16S rRNA sequences revealed an increased number of Lactobacillus and Streptococcus, and decreased number of Aeromonas at genus level in the distal intestine of marron fed FPBM. Marron fed FPBM showed up-regulated expression of IL-8, IL-10, and IL-17F genes in the distal intestine. Significantly (P < 0.05) increased lysozyme and phagocytic activity, and higher survival was found in marron fed FPBM following a bacterial challenge with Vibrio mimicus. Therefore, it is concluded that FPBM is beneficial to marron in terms of microbial community, immune-related cytokines and disease resistance against V. mimicus.

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Extract from the fermented soybean product Natto inhibits Vibrio biofilm formation and reduces shrimp mortality from Vibrio harveyi infection

Cited by 30 publications

References 33 publications

Analysis of microbiota in the stomach and midgut of two penaeid shrimps during probiotic feeding

Analysis of microbiota in the stomach and midgut of two penaeid shrimps during probiotic feeding

The role of rpoS in the regulation of Vibrio alginolyticus virulence and the response to diverse stresses

Fermented Animal Source Protein as Substitution of Fishmeal on Intestinal Microbiota, Immune-Related Cytokines and Resistance to Vibrio mimicus in Freshwater Crayfish (Cherax cainii)

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