Extracorporeal shock wave treatment protects skin flaps against ischemia–reperfusion injury

Reichenberger, M; Heimer, Sina; Schaefer, Amelia; Laß, Ulrike; Gebhard, Martha Maria; Germann, Günter; Engel, Holger; Köllensperger, Eva; Leimer, Uwe; Mueller, Wolf

doi:10.1016/j.injury.2011.11.019

Cited by 30 publications

(16 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…16 However, ischemia during surgery is unavoidable, and restoration of blood flow leads to tissue damage referred to as ischemiareperfusion injury. 17 In the present study, we found that muscone enhances random skin flap survival, associated with neovascularization, remission of tissue edema, and attenuated ischemia-reperfusion injury.…”

Section: Discussionsupporting

confidence: 58%

Effects of Muscone on Random Skin Flap Survival in Rats

Kailiang

Zhang

Lin

et al. 2015

J reconstr Microsurg

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 58%

Effects of Muscone on Random Skin Flap Survival in Rats

Kailiang

Zhang

Lin

et al. 2015

J reconstr Microsurg

View full text Add to dashboard Cite

show abstract

“…Postischemic PACE conditioning significantly downregulated expression of all assessed cytokines and chemokines (TNFα, IL‐6, IL‐1α, IL‐1β, GM‐CSF, CCL3, CCL4, and CXCL4) compared to both the ischemic and nonischemic controls. This observation is consistent with the results of previously published studies 3, 5–7. Davis et al presented the suppressive effect of ESWT on the early inflammatory response in a burn wound murine model.…”

Section: Discussionsupporting

confidence: 93%

“…Furthermore, as shown in our previous studies, shock waves stimulate angiogenesis by increasing expression of VEGF and eNOS in the nonischemic and ischemic cremaster muscle models 8, 9. Our observations are in line with the findings of other authors 5–7. Although reperfusion after ischemia is mandatory to prevent tissue necrosis, it elicits a microvascular response similar to inflammation.…”

supporting

confidence: 92%

“…ESWT mechanism of action can be described as a microstress applied to the treated tissue, inducing the release of growth factors and cytokines, ultimately leading to an accelerated healing process. There is a growing body of evidence within the literature, suggesting that ESWT can be successfully applied to protect various skin flaps including free flaps, which are commonly used in plastic and reconstructive surgery 5–7. This protection against ischemia/reperfusion injury (IRI) occurs through the suppression of inducible nitric oxide synthase (iNOS) and 8‐hydroxyguanosine (8‐OG), which are byproducts of ischemia 7.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Pre‐ and postischemic pulsed acoustic cellular expression conditioning modulates expression of inflammation factors in cremaster ischemia/reperfusion injury model

et al. 2012

View full text Add to dashboard Cite

Pulsed acoustic cellular expression (PACE) is a treatment that applies focused acoustic shock waves to promote tissue healing. The aim of this study was to assess the effect of PACE treatment on inflammatory responses in a cremaster muscle ischemia/reperfusion injury model. Seventeen cremaster muscle flaps were evaluated in four groups: nonischemic controls (n = 5), 5-hour ischemia controls (n = 4), preischemic (5-hour) PACE conditioning (n = 4), and postischemic (5-hour) PACE conditioning (n = 4). The expression of proinflammatory cytokines (TNFα, IL-6, IL-1α, IL-1β, GM-CSF) and chemokines (CCL3, CCL4, CXCL4) was assessed using TaqMan® real-time PCR. Expression of ELAM-1, VCAM-1, and ICAM-1 was assessed by immunostaining. Preischemic PACE conditioning upregulated expression of IL-6, CCL3, CCL4, and CXCL4, and downregulated expression of TNFα, GM-CSF, and IL-1α. Postischemic PACE conditioning significantly decreased expression of all evaluated genes. Pre- and postischemic PACE conditioning decreased expression of ELAM-1 and ICAM-1. Results of the study indicate that application of PACE conditioning may have a beneficial effect on the recovery of tissues subjected to the ischemia/reperfusion injury. Postischemic PACE conditioning revealed anti-inflammatory effect as confirmed by decreased expression of inflammatory cytokines, chemokines, and cell adhesion molecules (ELAM-1 and ICAM-1) that are responsible for leukocyte recruitment into ischemic tissues. Hence, PACE therapy may be used effectively in clinical practice as a convenient therapeutic strategy to protect tissues against ischemia/reperfusion related injury after microsurgical procedures of free tissue transfers.

show abstract

“…[1516] It can also promote the formation of new blood vessels, promote soft tissue repair and regeneration, and relieve pain. [17181920212223]…”

Section: Introductionmentioning

confidence: 99%

Effects of Roughly Focused Extracorporeal Shock Waves Therapy on the Expressions of Bone Morphogenetic Protein-2 and Osteoprotegerin in Osteoporotic Fracture in Rats

Huang

et al. 2016

Chinese Medical Journal

View full text Add to dashboard Cite

Background:Roughly focused extracorporeal shock waves therapy (ESWT) is characterized by a wide focal area, a large therapy zone, easy positioning, and less pain during treatment. The purpose of this study was to investigate the effects of roughly focused ESWT on the expression of osteoprotegerin (OPG) and bone morphogenetic protein-2 (BMP-2) in osteoporotic fractures in rats.Methods:Seventy-two female Sprague-Dawley (SD) rats, 3 months old, were divided into sham-operated group (n = 6) and an ovariectomized (OVX) group (n = 66). Sixty OVX SD rats were used as a model of double proximal tibial osteotomy and inner fixation. The osteotomy site in the left tibia was treated with roughly focused ESWT once at an energy density of 0.26 mJ/mm2, 60 doses/min, and 2000 pact quantities. The contralateral right tibia was left untreated and served as a control. Expression of OPG and BMP-2 in the callus of the osteoporotic fracture area was assessed using immunohistochemistry, real-time polymerase chain reaction (PCR), and Western blotting analysis.Results:Bone mineral density (BMD) at the proximal tibia, femur, and L5 spine was significantly reduced after ovariectomy. BMD of proximal tibia was 12.9% less in the OVX group than that in the sham-operated group. Meanwhile, bilateral oophorectomy resulted in a lower trabecular bone volume fraction (BV/TV) in the proximal tibia of the sham-OVX animals. Three months after bilateral oophorectomy, BV/TV was 14.29% of baseline BV/TV in OVX legs versus 45.91% in the sham-OVX legs (P < 0.001). These data showed that the SD rats became a suitable model of osteoporosis, 3 months after they were OVX. Immunohistochemical analysis showed higher levels of BMP-2 and OPG expression in the treatment group than those in the control group. Compared with the contralateral controls, decreased expression of OPG and BMP-2 at 3 days after roughly focused ESWT, followed by a later increase at 7 days, was indicated by real-time PCR and Western blotting analysis. The OPG messenger RNA (mRNA) expression levels peaked at 6 weeks after the shock wave treatment, paired with a much earlier (at 4 weeks) increase of BMP-2, and declined close to normal at 8 weeks.Conclusions:Roughly focused ESWT may promote the expression of OPG and BMP-2 in the osteoporotic fracture area in rats. BMP-2 and OPG may act synergistically and may lead to a significant enhancement of bone formation and remodeling.

show abstract

Extracorporeal shock wave treatment protects skin flaps against ischemia–reperfusion injury

Cited by 30 publications

References 34 publications

Effects of Muscone on Random Skin Flap Survival in Rats

Effects of Muscone on Random Skin Flap Survival in Rats

Pre‐ and postischemic pulsed acoustic cellular expression conditioning modulates expression of inflammation factors in cremaster ischemia/reperfusion injury model

Effects of Roughly Focused Extracorporeal Shock Waves Therapy on the Expressions of Bone Morphogenetic Protein-2 and Osteoprotegerin in Osteoporotic Fracture in Rats

Contact Info

Product

Resources

About