Extracorporeal circulation increases proliferation in the intestinal mucosa in a large animal model

Keschenau, Paula R.; Ribbe, Stefanie; Tamm, Miriam; Hanssen, Sebastiaan J.; Tolba, René; Jacobs, Michael J.; Kalder, Johannes

doi:10.1016/j.jvs.2015.05.043

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References 28 publications

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“…Studies are needed to elucidate the nonbiological and nonphysiological effects of ECLS. Although ECLS models in large animals, such as porcine models [6, 7], are well established because of the equipment, small animal models still have the potential for research in resuscitation studies. Rodent models are more economical when a high number of experiments must be performed.…”

Section: Introductionmentioning

confidence: 99%

Investigation of the pathophysiology of cardiopulmonary bypass using rodent extracorporeal life support model

Chang

Luo

et al. 2017

BMC Cardiovasc Disord

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BackgroundExtracorporeal life support (ECLS) systems are life-saving devices used for treating patients with severe cardiopulmonary failure. In this study, we implemented a rat model of ECLS without the administration of inotropes or vasopressors.MethodsThe rats underwent 5 min of untreated asphyxial cardiac arrest and were resuscitated by ECLS for 30 min. The right external jugular vein and right femoral artery were separately cannulated to the ECLS outflow and inflow, respectively. Thereafter, ECLS was terminated, wounds were closed, and mechanical ventilation was provided for another 90 min. Subsequently, blood gas and hemodynamic analyses were performed. The plasma levels of C-reactive protein (CRP), interleukin (IL)-6, IL-10, and tumor necrosis factor-alpha (TNF-α) were measured 120 min after reperfusion.ResultsThe metabolic rate of lactate in the group of asphyxial cardiac arrest rescued by ECLS was slow; therefore, the pH at 120 min after reperfusion was significantly lower in this group than that in the group of normal rats treated with ECLS. The hemodynamic data showed no between-group differences. The plasma levels of CRP, IL-6, IL-10, and TNF-α increased after ECLS treatment.ConclusionsWe successfully established a rodent ECLS model, which might be a useful approach for studying the pathophysiology induced by ECLS under clinical conditions.Electronic supplementary materialThe online version of this article (doi:10.1186/s12872-017-0558-6) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Investigation of the pathophysiology of cardiopulmonary bypass using rodent extracorporeal life support model

Chang

Luo

et al. 2017

BMC Cardiovasc Disord

View full text Add to dashboard Cite

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Extracorporeal circulation increases proliferation in the intestinal mucosa in a large animal model

Abstract: Mucosal proliferation is the early repair mechanism of the limited small intestinal ischemia/reperfusion injury after DAP. Furthermore, the extensive surgical trauma shifted the mucosal apoptosis into an advanced state.

Cited by 1 publication

References 28 publications

Investigation of the pathophysiology of cardiopulmonary bypass using rodent extracorporeal life support model

Investigation of the pathophysiology of cardiopulmonary bypass using rodent extracorporeal life support model

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