Extracellular vesicles released by fibroblasts undergoing H-Ras induced senescence show changes in lipid profile

Buratta, Sandra; Urbanelli, Lorena; Sagini, Krizia; Giovagnoli, Stefano; Caponi, Silvia; Fioretto, D.; Mitro, Nico; Caruso, Donatella; Emiliani, Carla

doi:10.1371/journal.pone.0188840

Cited by 60 publications

(68 citation statements)

References 50 publications

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“…This finding suggests that EVs may generally express a different lipid composition with respect to their originating cells. These results are in agreement with previous studies on lipid composition of EVs, demonstrating that vesicles from different cell types are enriched in CHO, PS and glycosphingolipids with respect to parental cells [22,29,30].…”

Section: Characterization Lipid Distribution and Fatty Acid Profilessupporting

confidence: 93%

The n-10 Fatty Acids Family in the Lipidome of Human Prostatic Adenocarcinoma Cell Membranes and Extracellular Vesicles

Ferreri

Sansone

Buratta

et al. 2020

Cancers

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A new pathway leading to the n-10 fatty acid series has been recently evidenced, starting from sapienic acid, a monounsaturated fatty acid (MUFA) resulting from the transformation of palmitic acid by delta-6 desaturase. Sapienic acid has attracted attention as a novel marker of cancer cell plasticity. Here, we analyzed fatty acids, including the n-10 fatty acid contents, and for the first time, compared cell membranes and the corresponding extracellular vesicles (EV) of two human prostatic adenocarcinoma cell lines of different aggressiveness (PC3 and LNCaP). The n-10 components were 9–13% of the total fatty acids in both cancer cell lines and EVs, with total MUFA levels significantly higher in EVs of the most aggressive cell type (PC3). High sapienic/palmitoleic ratios indicated the preference for delta-6 versus delta-9 desaturase enzymatic activity in these cell lines. The expressions analysis of enzymes involved in desaturation and elongation by qRT-PCR showed a higher desaturase activity in PC3 and a higher elongase activity toward polyunsaturated fatty acids than toward saturated fatty acids, compared to LNCaP cells. Our results improve the present knowledge in cancer fatty acid metabolism and lipid phenotypes, highlighting EV lipidomics to monitor positional fatty acid isomer profiles and MUFA levels in cancer.

show abstract

Section: Characterization Lipid Distribution and Fatty Acid Profilessupporting

confidence: 93%

The n-10 Fatty Acids Family in the Lipidome of Human Prostatic Adenocarcinoma Cell Membranes and Extracellular Vesicles

Ferreri

Sansone

Buratta

et al. 2020

Cancers

Self Cite

View full text Add to dashboard Cite

show abstract

“…This finding suggests that EVs may generally express a different lipid composition with respect to their originating cells. These results are in agreement with previous studies on lipid composition of EVs demonstrating that vesicles from different cell types are enriched in CHO, PS and glycosphingolipids with respect to parental cells [21,28,29]. The EVs fatty acid composition of PS, PE, PC was determined as described in Materials and methods, and Table 2 shows the % relative quantities (% rel.…”

Section: Characterization Lipid Distribution and Fatty Acid Profilessupporting

confidence: 89%

The n-10 Fatty Acids Family in the Lipidome of Human Prostatic Adenocarcinoma Cell Membranes and Extracellular Vesicles

Ferreri¹,

Sansone²,

Buratta³

et al. 2020

Preprint

Self Cite

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A new pathway leading to the n-10 fatty acid series has been recently evidenced, starting from sapienic acid - a monounsaturated fatty acid (MUFA) resulting from the transformation of palmitic acid by delta-6 desaturase. Sapienic acid attracts attention as novel marker of cancer cell plasticity. Here, we analyzed fatty acids including the n-10 fatty acid contents, and compared for the first time cell membranes and the corresponding extracellular vesicles (EV) of two human prostatic adenocarcinoma cell lines of different aggressiveness (PC3 and LNCaP). The n-10 components were 9-13% of the total fatty acids in both cancer cell lines and EVs, with total MUFA levels significantly higher in EVs of the most aggressive cell type (PC3). High sapienic/palmitoleic ratios indicated the preference for delta-6 vs. delta-9 desaturase enzymatic activity in these cell lines. The expressions analysis of enzymes involved in desaturation and elongation by qRT-PCR showed a higher desaturase activity in PC3 and a higher elongase activity toward polyunsaturated fatty acids than toward saturated fatty acids, compared to LNCaP cells. Our results improve the present knowledge in cancer fatty acid metabolism and lipid phenotypes, highlighting EV lipidomics to monitor positional fatty acid isomer profiles and MUFA levels in cancer.

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“…Less is known about Cer1P, a phosphorylated ceramide only present in minute quantities in whole cells, and ST, a sphingolipid present in most cell membranes and enriched in myelin sheaths. Both however have been observed to be secreted by cells into culture media (54), with ST being preferentially secreted in extracellular vesicles from senescent fibroblasts (55). ST also induces inflammation in microglia (56), and predisposes to seizures if accumulated in neurons (57).…”

Section: Discussionmentioning

confidence: 99%

Adiponectin and related C1q/TNF-related proteins bind selectively to anionic phospholipids and sphingolipids

Bian

Lim

et al. 2019

Preprint

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Adiponectin (also known as Acrp30) is a well-known adipokine associated with protection from cardiovascular disease, insulin resistance, and inflammation. Though multiple studies have investigated the mechanism of action of adiponectin and its relationship with tissue ceramide levels, several aspects of adiponectin biology remain unexplained, including its high circulating levels, tendency to oligomerize, and marked structural similarity to the opsonin C1q. Given the connection between adiponectin and ceramide metabolism, and the lipid-binding properties of C1q, we hypothesized that adiponectin may function as a lipid binding protein. Indeed, we found that recombinant adiponectin bound to various anionic phospholipids and sphingolipids, including phosphatidylserine, ceramide-1-phosphate, and sulfatide. The globular head-domain of adiponectin was necessary and sufficient for lipid binding. Adiponectin oligomerization was also observed to be critical for efficient lipid binding. In addition to lipids in liposomes, adiponectin bound LDL in an oligomerization-dependent manner. Other C1qTNF-related protein (CTRP) family members Cbln1, CTRP1, CTRP5, and CTRP13 also bound similar target lipids in 2 liposomes. These findings suggest that adiponectin and other CTRP family members may not only function as classical hormones, but also as lipid binding opsonins or carrier proteins.

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Extracellular vesicles released by fibroblasts undergoing H-Ras induced senescence show changes in lipid profile

Cited by 60 publications

References 50 publications

The n-10 Fatty Acids Family in the Lipidome of Human Prostatic Adenocarcinoma Cell Membranes and Extracellular Vesicles

The n-10 Fatty Acids Family in the Lipidome of Human Prostatic Adenocarcinoma Cell Membranes and Extracellular Vesicles

The n-10 Fatty Acids Family in the Lipidome of Human Prostatic Adenocarcinoma Cell Membranes and Extracellular Vesicles

Adiponectin and related C1q/TNF-related proteins bind selectively to anionic phospholipids and sphingolipids

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