Extracellular Vesicles Reflect the Efficacy of Wheatgrass Juice Supplement in Colon Cancer Patients During Adjuvant Chemotherapy

Avisar, Adva; Cohen, Miri; Brenner, Benjamin; Bronshtein, Tomer; Machluf, Marcelle; Bar-Sela, Gil; Aharon, Anat

doi:10.3389/fonc.2020.01659

Cited by 18 publications

(15 citation statements)

References 43 publications

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“…EVs were isolated as previously described [ 47 ]. In brief, blood samples were collected in tubes containing sodium citrate (3.2%) and EDTA; differential centrifugations were performed according to the current gold standard for EV isolation [ 48 ].…”

Section: Methodsmentioning

confidence: 99%

Extracellular Vesicle MicroRNA That Are Involved in β-Thalassemia Complications

Levin

Koren

Rebibo-Sabbah

et al. 2021

IJMS

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Beta thalassemia major (βT) is a hereditary anemia characterized by transfusion-dependency, lifelong requirement of chelation, and organ dysfunction. MicroRNA (miRNA) can be packed into extracellular vesicles (EVs) that carry them to target cells. We explored EV-miRNA in βT and their pathophysiologic role. Circulating EVs were isolated from 35 βT-patients and 15 controls. EV miRNA was evaluated by nano-string technology and real-time quantitative polymerase chain reaction (RT-qPCR). We explored effects of EVs on cell culture proliferation, apoptosis, and signal transduction. Higher amounts of small EV (exosomes) were found in patients than in controls. The expression of 21 miRNA was > two-fold higher, and of 17 miRNA < three-fold lower in βT-EVs than control-EVs. RT-qPCR confirmed differential expression of six miRNAs in βT, particularly miR-144-3p, a regulator of erythropoiesis. Exposure of endothelial, liver Huh7, and pancreatic 1.1B4 cells to βT-EVs significantly reduced cell viability and increased cell apoptosis. βT-EV-induced endothelial cell apoptosis involved the MAPK/JNK signal-transduction pathway. In contrast, splenectomized βT-EVs induced proliferation of bone marrow mesenchymal stem cells (BM-MSC). In summary, the miR-144-3p was strongly increased; βT-EVs induced apoptosis and decreased endothelial, pancreatic, and liver cell survival while supporting BM-MSC proliferation. These mechanisms may contribute to βT organ dysfunction and complications.

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Section: Methodsmentioning

confidence: 99%

Extracellular Vesicle MicroRNA That Are Involved in β-Thalassemia Complications

Levin

Koren

Rebibo-Sabbah

et al. 2021

IJMS

Self Cite

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“…In addition, other factors such as food compounds ( 183 , 184 ), temperature ( 185 , 186 ), radiotherapy ( 187 ) and chemotherapy ( 188 , 189 )affect intercellular communication mechanisms by mediating exosome release process, which allows exosomes to perform different functions and then contributes to the diagnosis and treatment of diseases.…”

Section: Exogenous Factors Modulate Exosome Releasementioning

confidence: 99%

Current perspectives on clinical use of exosomes as novel biomarkers for cancer diagnosis

Chen

Huang

et al. 2022

Front. Oncol.

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Exosomes are a heterogeneous subset of extracellular vesicles (EVs) that biogenesis from endosomes. Besides, exosomes contain a variety of molecular cargoes including proteins, lipids and nucleic acids, which play a key role in the mechanism of exosome formation. Meanwhile, exosomes are involved with physiological and pathological conditions. The molecular profile of exosomes reflects the type and pathophysiological status of the originating cells so could potentially be exploited for diagnostic of cancer. This review aims to describe important molecular cargoes involved in exosome biogenesis. In addition, we highlight exogenous factors, especially autophagy, hypoxia and pharmacology, that regulate the release of exosomes and their corresponding cargoes. Particularly, we also emphasize exosome molecular cargoes as potential biomarkers in liquid biopsy for diagnosis of cancer.

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“…Besides, some exosomal-derived miRNAs have been detected that play a role in regulating angiogenic transcription factors, promoting angiogenesis and MDR in CRC [ 4 ]. Studies on rich-nutrition wheatgrass juice (WGJ) that extracted from plant Triticum aestivum, revealed post-chemotherapy improvement and modifying tumor associated microvesicles including exosomes properties by daily consumption of WGJ in CRC patient undergoing chemotherapy [ 29 ]. Moreover, exosomes containing miR-934 can shift the macrophages into M2 phase and promote liver metastasis of colorectal cancer which is one of the deadliest consequences of CRC [ 30 ].…”

Section: Drug Resistance Mechanisms In Crcmentioning

confidence: 99%

Recent advances in targeted drug delivery systems for resistant colorectal cancer

et al. 2022

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Colorectal cancer (CRC) is one of the deadliest cancers in the world, the incidences and morality rate are rising and poses an important threat to the public health. It is known that multiple drug resistance (MDR) is one of the major obstacles in CRC treatment. Tumor microenvironment plus genomic instability, tumor derived exosomes (TDE), cancer stem cells (CSCs), circulating tumor cells (CTCs), cell-free DNA (cfDNA), as well as cellular signaling pathways are important issues regarding resistance. Since non-targeted therapy causes toxicity, diverse side effects, and undesired efficacy, targeted therapy with contribution of various carriers has been developed to address the mentioned shortcomings. In this paper the underlying causes of MDR and then various targeting strategies including exosomes, liposomes, hydrogels, cell-based carriers and theranostics which are utilized to overcome therapeutic resistance will be described. We also discuss implication of emerging approaches involving single cell approaches and computer-aided drug delivery with high potential for meeting CRC medical needs.

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Extracellular Vesicles Reflect the Efficacy of Wheatgrass Juice Supplement in Colon Cancer Patients During Adjuvant Chemotherapy

Cited by 18 publications

References 43 publications

Extracellular Vesicle MicroRNA That Are Involved in β-Thalassemia Complications

Extracellular Vesicle MicroRNA That Are Involved in β-Thalassemia Complications

Current perspectives on clinical use of exosomes as novel biomarkers for cancer diagnosis

Recent advances in targeted drug delivery systems for resistant colorectal cancer

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