Extracellular vesicles of blood plasma: content, origin, and properties

Panteleev, Mikhail A.; Абаева, А. А.; Баландина, А. Н.; Belyaev, Aleksey V.; Nechipurenko, Dmitry; Obydennyi, Sergey I.; Sveshnikova, Anastasia N.; Shibeko, Alexey M.; Ataullakhanov, Fazoil I.

doi:10.1134/s1990747817030060

Cited by 10 publications

(10 citation statements)

References 45 publications

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“…However, apoH is a misnomer, also known as beta‐2‐glycoprotein 1, a protein that is associated with cardiolipin containing membranes rather than to lipoprotein particles. Plasma EV have diverse cellular origins and are thus heterogeneous in protein composition [67]. Among the 250 proteins that we detected in highly purified EV, many are associated with GO terms that are related to complement or immune regulation.…”

Section: Discussionmentioning

confidence: 99%

A novel three step protocol to isolate extracellular vesicles from plasma or cell culture medium with both high yield and purity

Zhang

Borg

Liaci

et al. 2020

J of Extracellular Vesicle

108

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Extracellular vesicles (EV) are membrane encapsulated nanoparticles that can function in intercellular communication, and their presence in biofluids can be indicative for (patho)physiological conditions. Studies aiming to resolve functionalities of EV or to discover EV-associated biomarkers for disease in liquid biopsies are hampered by limitations of current protocols to isolate EV from biofluids or cell culture medium. EV isolation is complicated by the >10 5-fold numerical excess of other types of particles, including lipoproteins and protein complexes. In addition to persisting contaminants, currently available EV isolation methods may suffer from inefficient EV recovery, bias for EV subtypes, interference with the integrity of EV membranes, and loss of EV functionality. In this study, we established a novel three-step non-selective method to isolate EV from blood or cell culture media with both high yield and purity, resulting in 71% recovery and near to complete elimination of unrelated (lipo)proteins. This EV isolation procedure is independent of illdefined commercial kits, and apart from an ultracentrifuge, does not require specialised expensive equipment.

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Section: Discussionmentioning

confidence: 99%

A novel three step protocol to isolate extracellular vesicles from plasma or cell culture medium with both high yield and purity

Zhang

Borg

Liaci

et al. 2020

J of Extracellular Vesicle

108

View full text Add to dashboard Cite

show abstract

“…It is unclear why these proteins are decreasing in plasma EVs of patients with more advanced tumors with a higher metastatic potential. It is important to note that plasma EVs consist not only of tumor-derived exosomes but also sEVs from different origins such as platelets ( 33 ), leukocytes, or adipose tissues ( 34 ). The high correlation of exosome number and BMI as shown in fig.…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of circulating extracellular vesicles identifies potential markers of breast cancer progression, recurrence, and response

Vinik

Ortega

Mills³

et al. 2020

Sci. Adv.

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Proteomic profiling of circulating small extracellular vesicles (sEVs) represents a promising, noninvasive approach for early detection and therapeutic monitoring of breast cancer (BC). We describe a relatively low-cost, fast, and reliable method to isolate sEVs from plasma of BC patients and analyze their protein content by semiquantitative proteomics. sEV-enriched fractions were isolated from plasma of healthy controls and BC patients at different disease stages before and after surgery. Proteomic analysis of sEV-enriched fractions using reverse phase protein array revealed a signature of seven proteins that differentiated BC patients from healthy individuals, of which FAK and fibronectin displayed high diagnostic accuracy. The size of sEVs was significantly reduced in advanced disease stage, concomitant with a stage-specific protein signature. Furthermore, we observed protein-based distinct clusters of healthy controls, chemotherapy-treated and untreated postsurgery samples, as well as a predictor of high risk of cancer relapse, suggesting that the applied methods warrant development for advanced diagnostics.

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“…The next step will be the validation of EV markers on plasma samples from patients with LC and CRC. In this case, putative LC and CRC biomarkers should be analyzed against the complex background of EVs originating from blood cells, i.e., platelets, erythrocytes, and leukocytes [ 52 ]. Previously, we studied EVs isolated from the blood plasma of healthy volunteers and used SRM analysis to determine the levels of the exosomal markers CD9, CD82, and HSPA8 [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

Proteomic Approach for Searching for Universal, Tissue-Specific, and Line-Specific Markers of Extracellular Vesicles in Lung and Colorectal Adenocarcinoma Cell Lines

Novikova

Shushkova

Farafonova

et al. 2020

IJMS

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Tumor-derived extracellular vesicles (EVs), including exosomes, contain proteins that mirror the molecular landscape of producer cells. Being potentially detectible in biological fluids, EVs are of great interest for the screening of cancer biomarkers. To reveal universal, tissue-specific, and line-specific markers, we performed label-free mass spectrometric profiling of EVs originating from the human colon cancer cell lines Caco-2, HT29, and HCT-116, as well as from the lung cancer cell lines NCI-H23 and A549. A total of 651 proteins was identified in the EV samples using at least two peptides. These proteins were highly enriched in exosome markers. We found 11 universal, eight tissue-specific, and 29 line-specific markers, the levels of which were increased in EVs compared to the whole lysates. The EV proteins were involved in the EGFR, Rap1, integrin, and microRNA signaling associated with metastasis and cancer progression. An EV protein-based assay could be developed as a liquid biopsy tool.

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Extracellular vesicles of blood plasma: content, origin, and properties

Cited by 10 publications

References 45 publications

A novel three step protocol to isolate extracellular vesicles from plasma or cell culture medium with both high yield and purity

A novel three step protocol to isolate extracellular vesicles from plasma or cell culture medium with both high yield and purity

Proteomic analysis of circulating extracellular vesicles identifies potential markers of breast cancer progression, recurrence, and response

Proteomic Approach for Searching for Universal, Tissue-Specific, and Line-Specific Markers of Extracellular Vesicles in Lung and Colorectal Adenocarcinoma Cell Lines

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