Extracellular Vesicles Mediate the Embryonic-Maternal Paracrine Communication

Saadeldin, Islam M.

doi:10.1007/978-981-15-6599-1_3

Cited by 3 publications

(3 citation statements)

References 78 publications

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“…Oviduct EV characterization identified proteins with critical roles in the gamete/embryo-oviduct interactions, such as OVGP1, heat shock protein (HSP) 90, HSPA8, HSP70, gelsolin and ezrin ( 22 ). In addition, spermatozoa undergo several modifications in the female reproductive tract for the capacitation process to acquire a complete fertilizing ability ( 52 , 53 ). Porcine oviduct EVs attach to sperm membranes ( 54 ) and participate in the maintenance of sperm viability and reducing motility, functions associated with the oviduct sperm reservoir ( 36 , 55 ).…”

Section: Discussionmentioning

confidence: 99%

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Oviduct epithelial cell‑derived extracellular vesicles promote the developmental competence of IVF porcine embryos

et al. 2023

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Assisted reproductive technology has increased the efficiency of animal reproduction. However, polyspermy is a significant limitation of porcine in vitro fertilization (IVF). Therefore, reducing the polyspermy rate and improving monospermic embryos is crucial. Recent studies have reported that oviductal fluid, along with its contents of extracellular vesicles (EVs), enhanced the fertilization process and supported embryo development. Consequently, the present study investigated the effects of porcine oviduct epithelial cells (OEC-EVs) on sperm-oocyte interactions during porcine IVF and evaluated in vitro embryo developmental competence outcomes. During IVF embryo development, the cleavage rate was significantly higher in the group treated with 50 ng/ml OEC-EVs compared with the control group (67.6±2.5 vs. 57.3±1.9; P<0.05). Furthermore, the OEC-EV group had significantly more embryos (16.4±1.2 vs. 10.2±0.8; P<0.05), and the polyspermy rate significantly decreased (32.9±2.5 vs. 43.8±3.1; P<0.05) compared with that of the control group. Additionally, the fluorescence intensities of cortical granules (3.56±0.47 vs. 2.15±0.24; P<0.05) and active mitochondria (8.14±0.34 vs. 5.96±0.38; P<0.05) were significantly higher in the OEC-EV group compared with those in the control group. In conclusion, OEC-EV adsorption and penetration crosstalk between sperm and oocytes was observed. OEC-EV treatment was demonstrated to significantly improve the concentration and distribution of cortical granules in oocytes. Furthermore, OEC-EVs also increased oocyte mitochondrial activity, reduced polyspermy and increased the IVF success rate.

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Section: Discussionmentioning

confidence: 99%

“…Oviduct EVs also regulate plasma membrane Ca 2+ -ATPase 1 to promote sperm motility ( 7 ). Moreover, proteins and miRNAs in OEC-EVs may enhance cross-talk in oocyte fertilization ( 53 , 56 ). Ferraz et al ( 57 ) reported that oviductal EV supplementation to fresh epididymal spermatozoa enhanced sperm motility and functions.…”

Section: Discussionmentioning

confidence: 99%

Oviduct epithelial cell‑derived extracellular vesicles promote the developmental competence of IVF porcine embryos

et al. 2023

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“…Recently, several studies have shown that extracellular vesicles (EVs) can act as mediators of reproductive physiology in males ( 6 – 9 ). EVs are tiny vesicles including exosomes and microvesicles and are secreted by many cells that carry nucleic acid and protein cargo to help cell-to-cell communication; they play an important crucial role in reproduction physiology ( 10 – 12 ).…”

Section: Introductionmentioning

confidence: 99%

Shuttle Transfer of mRNA Transcripts via Extracellular Vesicles From Male Reproductive Tract Cells to the Cumulus–Oocyte Complex in Rabbits (Oryctolagus cuniculus)

et al. 2022

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Semen is known to contain an ovulation-inducing factor (identified as a nerve growth factor, NGF) that shows a significant increase in ovulation after semen deposition in induced ovulatory species. However, the interplay between the male reproductive tract cells and oocyte maturation through messenger RNA (mRNA) cargo is yet to be investigated. Extracellular vesicles (EVs) from the primary culture of rabbit prostate (pEVs), epididymis (eEVs), and testis (tEVs) were isolated to examine their contents for several mRNA transcripts through relative quantitative PCR (RT-qPCR). The expressions of NGF, neurotrophin (NTF3), vascular endothelial growth factor A (VEGFA), A disintegrin and metalloprotease 17 (ADAM17), midkine (MDK), kisspeptin (KISS1), and gonadotrophin-releasing hormone (GNRH1) were examined in isolated EVs. EVs were characterized through transmission electron microscopy. EV uptake by cumulus cell culture was confirmed through microscopic detection of PKH26-stained EVs. Furthermore, the effects of pEVs, eEVs, and tEVs were compared with NGF (10, 20, and 30 ng/ml) supplementation on oocyte in vitro maturation (IVM) and transcript expression. KISS1, NTF3, MDK, ADAM17, GAPDH, and ACTB were detected in all EV types. GNRH1 was detected in tEVs. NGF was detected in pEVs, whereas VEGFA was detected in eEVs. pEVs, eEVs, and 20 ng/ml NGF showed the highest grade of cumulus expansion, followed by tEVs and 10 ng/ml NGF. Control groups and 30 ng/ml NGF showed the least grade of cumulus expansion. Similarly, first polar body (PB) extrusion was significantly increased in oocytes matured with eEVs, pEVs, tEVs, NGF20 (20 ng/ml NGF), NGF10 (10 ng/ml NGF), control, and NGF30 (30 ng/ml NGF). Additionally, the expression of NGFR showed a 1.5-fold increase in cumulus cells supplemented with eEVs compared with the control group, while the expression of PTGS2 (COX2) and NTRK showed 3-fold and 5-fold increase in NGF20-supplemented cumulus-oocyte complexes (COCs), respectively. Oocyte PMP15 expression showed a 1.8-fold increase in IVM medium supplemented with eEVs. Additionally, oocyte NGFR and NTRK expressions were drastically increased in IVM medium supplemented with pEVS (3.2- and 1.6-fold, respectively) and tEVs (4- and 1.7-fold, respectively). This is the first report to examine the presence of mRNA cargo in the EVs of male rabbit reproductive tract cells that provides a model for the stimulation of female rabbits after semen deposition.

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