Extracellular vesicles in food: Experimental evidence of their secretion in grape fruits

Pérez‐Bermúdez, Pedro; Blesa, Jesús; Castillo, José Miguel Soriano del; Marcilla, Antonio

doi:10.1016/j.ejps.2016.09.022

Cited by 87 publications

(74 citation statements)

References 61 publications

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“…These issues motivated researchers to explore alternative EV sources, in particular, food such as bovine milk and plants, which have the main advantage of being easily accessible, cost‐effective, and scalable . Since they are common components of our diet, milk and plants are also considered biocompatible and safe sources of vesicles .…”

Section: Methods For Improvement Of Upstream Ev Yieldmentioning

confidence: 99%

Scalable Production and Isolation of Extracellular Vesicles: Available Sources and Lessons from Current Industrial Bioprocesses

Paganini

Palmiero

Pócsfalvi

et al. 2019

Biotechnology Journal

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Potential applications of extracellular vesicles (EVs) are attracting increasing interest in the fields of medicine, cosmetics, and nutrition. However, the manufacturing of EVs is currently characterized by low yields. This limitation severely hampers progress in research at the laboratory and clinical scales, as well as the realization of successful and cost-effective EV-based products. Moreover, the high level of heterogeneity of EVs further complicates reproducible manufacturing on a large scale. In this review, possible directions toward the scalable production of EVs are discussed. In particular, two strategies are considered: i) the optimization of upstream unit operations and ii) the exploitation of well-established and mature technologies already in use in other industrial bioprocesses.

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Section: Methods For Improvement Of Upstream Ev Yieldmentioning

confidence: 99%

Scalable Production and Isolation of Extracellular Vesicles: Available Sources and Lessons from Current Industrial Bioprocesses

Paganini

Palmiero

Pócsfalvi

et al. 2019

Biotechnology Journal

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“…The choice of starting material and quantity should be carefully considered when purifying EVs. For example, medium should be collected from bacterial cultures in the exponential versus the stationary growth phase to reduce the amount of debris from dying cells, and extracellular plant (apoplastic) fluids should be used instead of plant fluids obtained by mechanical disruption of tissues as these will contain a mixture of extracellular and intracellular vesicles (Pérez-Bermúdez et al, 2017). EVs can also be isolated based on size by membrane filtration, i.e.…”

Section: Techniques For Ev Isolationmentioning

confidence: 99%

Functions of Extracellular Vesicles in Immunity and Virulence

Rybak

Robatzek

2019

Plant Physiol.

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“…These extracellular vesicles often contain DNA and regulatory RNA and proteins that can influence the function of recipient cells (Yáñez-Mó et al, 2015;Bitto et al, 2017;Pérez-Bermúdez et al, 2017). The abundant cyanobacterium Prochlorococcus as well as other photosynthetic bacteria have been observed to release vesicles into the ocean (Biller et al, 2014(Biller et al, , 2017.…”

Section: Stromules-a Source Of Plastid-derived Vesicles?mentioning

confidence: 99%