Extracellular vesicles from thyroid cancer harbor a functional machinery involved in extracellular matrix remodeling

Bravo-Miana, Rocío del Carmen; Soler, María Florencia; Ceschin, Danilo Guillermo; Royo, Félix; Negretti-Borga, Dana María; Azkargorta, Mikel; Elortza, Félix; Montesinos, María del Mar; Pellizas, Claudia G.; Falcón‐Pérez, Juan Manuel; Donadío, Ana Carolina

doi:10.1016/j.ejcb.2022.151254

Cited by 6 publications

(4 citation statements)

References 51 publications

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“…This distinctive pattern suggests a relationship between endocytosis and vesicle uptake, a phenomenon consistent with observations in other studies. [29][30][31] We also explored the anti-cancer effects of the NKderived vesicles. Caspase-mediated apoptosis, particularly via the perforin/granzyme and death receptor signaling (Fas/FasL) pathways, was identified as a crucial mechanism of vesicle-induced cell death in the results.…”

Section: Discussionmentioning

confidence: 99%

Cytochalasin B‐Induced Membrane Vesicles of NK Cells for Efficient Tumor Immunotherapy

Lee,

Kwon

2024

Advanced Therapeutics

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Extracellular vesicles (EVs) are crucial mediators of cell‐to‐cell communication and contain biological components such as lipids, proteins, and nucleic acids. EVs are studied for their crucial immunomodulatory roles, particularly in natural killer (NK) cells. NK vesicles, which share surface markers and biological components with the parental NK cells, are developed as anti‐cancer agents. However, their clinical application is hindered by their low productivity and an incomplete understanding of their functional mechanisms. In this study, EV‐mimicking vesicles are artificially induced from NK cells by cytochalasin B treatment. These cytochalasin B‐induced membrane vesicles (CIMVs) are 1.21‐fold more concentrated than isolated natural EVs from NK cells (NK‐EVs) and contain 1.66‐fold more proteins, including those with immunological activity against tumors. The induced ARF6‐positive microvesicles possess an immunological phenotype similar to that of the parental cells, while perforin, granzyme, and FasL proteins are more abundant compared to NK‐EVs. Administrating NK‐EVs and CIMVs to K562 and MCF‐7 tumor cells induces caspase‐dependent apoptosis, which leads to tumor cell cytotoxicity. These results significantly contribute to the understanding of the role of NK vesicles in cellular communication and immunity, and highlight the therapeutic potential of engineered NK‐EVs via their specific action on tumor cells.

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Section: Discussionmentioning

confidence: 99%

Cytochalasin B‐Induced Membrane Vesicles of NK Cells for Efficient Tumor Immunotherapy

Lee,

Kwon

2024

Advanced Therapeutics

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“…CD147, initially known as EMMPRIN (extracellular matrix metalloproteinase inducer), is a glycoprotein overexpressed in many types of cancers and associated with a poor prognosis. The same group recently confirmed the regulation of extracellular matrix remodeling by EVs isolated from thyroid cancer cell-fibroblast co-culture [ 35 ], illustrating EVs’ dynamic role.…”

Section: Evs In Tcmentioning

confidence: 90%

Role of Extracellular Vesicles in Thyroid Physiology and Diseases: Implications for Diagnosis and Treatment

2022

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Extracellular vesicles are spherical subcellular structures delimited by a lipid bilayer and released by most cells in the human body. They are loaded with a myriad of molecules (i.e., nucleic acids and proteins) depending on their cell of origin and provide the ability to transmit a message to surrounding or distant target cells. In several organs, including the thyroid, abundant recent literature reports that extracellular vesicles are responsible for intercellular communication in physiological and pathological processes, and that their utilization as a potential biomarker of pathological states (i.e., cancer, autoimmune diseases) or as therapeutic delivery vehicles promise clinical options. In this review, we present the current knowledge and understanding regarding the role of extracellular vesicles in developing thyroid diseases and diagnosis.

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“…Particularly, matrix metalloproteinase 2 is known to be a vital enzyme for the degradation of the ECM. Tumor invasion is the clinically tangible outcome of the process [ 253 ]. TAMs are also manifested as cooperating in the ECM remodeling process.…”

Section: Extracellular Vesicles and Cancer Stem Cells In The Tumor Mi...mentioning

confidence: 99%

Extracellular vesicles and cancer stem cells: a deadly duo in tumor progression

Tayanloo-Beik,

Eslami,

Sarvari

et al. 2024

Oncol.Rev.

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The global incidence of cancer is increasing, with estimates suggesting that there will be 26 million new cases and 17 million deaths per year by 2030. Cancer stem cells (CSCs) and extracellular vesicles (EVs) are key to the resistance and advancement of cancer. They play a crucial role in tumor dynamics and resistance to therapy. CSCs, initially discovered in acute myeloid leukemia, are well-known for their involvement in tumor initiation, progression, and relapse, mostly because of their distinct characteristics, such as resistance to drugs and the ability to self-renew. EVs, which include exosomes, microvesicles, and apoptotic bodies, play a vital role in facilitating communication between cells within the tumor microenvironment (TME). They have a significant impact on cellular behaviors and contribute to genetic and epigenetic changes. This paper analyzes the mutually beneficial association between CSCs and EVs, emphasizing their role in promoting tumor spread and developing resistance mechanisms. This review aims to investigate the interaction between these entities in order to discover new approaches for attacking the complex machinery of cancer cells. It highlights the significance of CSCs and EVs as crucial targets in the advancement of novel cancer treatments, which helps stimulate additional research, promote progress in ideas for cancer treatment, and provide renewed optimism in the effort to reduce the burden of cancer.

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Extracellular vesicles from thyroid cancer harbor a functional machinery involved in extracellular matrix remodeling

Cited by 6 publications

References 51 publications

Cytochalasin B‐Induced Membrane Vesicles of NK Cells for Efficient Tumor Immunotherapy

Cytochalasin B‐Induced Membrane Vesicles of NK Cells for Efficient Tumor Immunotherapy

Role of Extracellular Vesicles in Thyroid Physiology and Diseases: Implications for Diagnosis and Treatment

Extracellular vesicles and cancer stem cells: a deadly duo in tumor progression

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