Extracellular vesicles derived from Plasmodium-infected and non-infected red blood cells as targeted drug delivery vehicles

Borgheti-Cardoso, Lívia Neves; Kooijmans, Sander A.A.; Chamorro, Lucía Gutiérrez; Biosca, Arnau; Lantero, Elena; Ramírez, Miriam; Avalos-Padilla, Yunuen; Crespo, Isabel; Fernández, Irene; Fernández-Becerra, Carmen; Portillo, Hernando A. del; Fernàndez‐Busquets, Xavier

doi:10.1016/j.ijpharm.2020.119627

Cited by 30 publications

(42 citation statements)

References 75 publications

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“…Altogether, these findings implicate iREVs as facilitators of immunopathology and vascular dysfunction in malaria by virtue of the biomolecular cargo they carry. Several P. falciparum in vitro studies have comprehensively analysed the protein [ 100 , 113 , 114 ], nucleic acid [ 100 , 102 , 112 , 115 , 116 ], and lipid content of iREVs [ 117 , 118 ] (Table 3 ). Independent proteomic analyses of EVs from rodent P. yoelii infections [ 54 , 55 ] and plasma derived EVs from P. vivax infections of liver-chimeric humanized mice [ 56 ] have also been performed (Table 2 ).…”

Section: The Pathogenesis Of Malaria: a Potential Role For Evsmentioning

confidence: 99%

“…When monocytes were treated with PfEMP1-carrying iREVs, genes involved in response to stress and cytokine stimulus, as well as response to defence, were not upregulated, whereas following treatment with PfEMP1-deficient iREVs, monocytes showed a significant upregulation of these genes [ 114 ]. In P. berghei rodent infection, compared to iREVs from HRF and Ef-1α knock-out mice, wild type sourced iREVs efficiently inhibited T cell proliferation in vitro and in vivo [ 118 ]. Over 3 decades ago, malaria antigens were reported to evade immune responses by inducing humoral and cell-mediated immunosuppression directly, albeit through poorly understood mechanisms [ 125 , 126 ].…”

Section: The Biology Of Malaria: Novel Features From An Ev Perspectivementioning

confidence: 99%

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Extracellular vesicles in malaria: an agglomeration of two decades of research

Opadokun

Rohrbach

2021

Malar J

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Malaria is a complex parasitic disease, caused by Plasmodium spp. More than a century after the discovery of malaria parasites, this disease continues to pose a global public health problem and the pathogenesis of the severe forms of malaria remains incompletely understood. Extracellular vesicles (EVs), including exosomes and microvesicles, have been increasingly researched in the field of malaria in a bid to fill these knowledge gaps. EVs released from Plasmodium-infected red blood cells and other host cells during malaria infection are now believed to play key roles in disease pathogenesis and are suggested as vital components of the biology of Plasmodium spp. Malaria-derived EVs have been identified as potential disease biomarkers and therapeutic tools. In this review, key findings of malaria EV studies over the last 20 years are summarized and critically analysed. Outstanding areas of research into EV biology are identified. Unexplored EV research foci for the future that will contribute to consolidating the potential for EVs as agents in malaria prevention and control are proposed.

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Section: The Pathogenesis Of Malaria: a Potential Role For Evsmentioning

confidence: 99%

Section: The Biology Of Malaria: Novel Features From An Ev Perspectivementioning

confidence: 99%

Extracellular vesicles in malaria: an agglomeration of two decades of research

Opadokun

Rohrbach

2021

Malar J

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“…Furthermore, EVs can be secreted from all tissues and organs in both health and disease conditions, and these include exosomes, epididymosomes, prostasomes, ectosomes, apoptotic bodies, microvesicles, and more recently oncosomes [ 96 , 97 , 108 ], and analysis of the EV contents can provide information about differentiation/functional state of parental cells [ 116 ]. These EVs have been found in all biological fluids including plasma, urine, saliva, semen, bronchoalveolar lavage, bile, ascitic fluid, breast milk, cerebrospinal fluid, and RBCs [ 117 , 118 , 119 ]. The first observation of EVs was in platelet-free serum in 1946 [ 120 ].…”

Section: Red Blood Cells Extracellular Vesiclementioning

confidence: 99%

“…Several investigators have reported about the isolation and the characterization of EVs or microparticles from red blood cells [ 136 , 137 , 138 , 139 ] and the release mechanism of MVs in RBCs, and their properties in response to ATP depletion, oxidative stress, and storage were also suggested [ 140 , 141 , 142 ]. More recently, isolation of EVs derived from plasmodium-infected and non-infected RBCs as targeted drug delivery vehicles has been reported [ 117 ]. In addition, an increased level of RBCs-derived MVs in the circulating blood of patients with SCA, thalassemia, and glucose-6-phosphate-deficiency (G6PD) have been observed [ 143 , 144 ].…”

Section: Red Blood Cells Extracellular Vesiclementioning

confidence: 99%

“…The advantages of using EVs over synthetic delivery systems including (i) greater stability in the blood because of their natural surface composition [ 117 , 254 ]; (ii) possibly better protection of the encapsulated cargo due to a proteo-lipid architecture [ 255 ]; (iii) endogenous cell and tissue targeting features afforded by their adhesion molecules and surface ligands [ 254 ]; and (iv) higher biocompatibility allowing improved permeability through biological barriers, including the blood–brain barrier [ 173 ]. It has been suggested that EVs may serve as novel tools for various therapeutic approaches, including (a) anti-tumor therapy; (b) pathogen vaccination; (c) immune-modulatory and regenerative therapies; and (d) drug delivery [ 236 , 256 ].…”

Section: Extracellular Vesicles Clinical Application and Therapeutic Potentialmentioning

confidence: 99%

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The Mystery of Red Blood Cells Extracellular Vesicles in Sleep Apnea with Metabolic Dysfunction

Sanz-Rubio

2021

IJMS

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Sleep is very important for overall health and quality of life, while sleep disorder has been associated with several human diseases, namely cardiovascular, metabolic, cognitive, and cancer-related alterations. Obstructive sleep apnea (OSA) is the most common respiratory sleep-disordered breathing, which is caused by the recurrent collapse of the upper airway during sleep. OSA has emerged as a major public health problem and increasing evidence suggests that untreated OSA can lead to the development of various diseases including neurodegenerative diseases. In addition, OSA may lead to decreased blood oxygenation and fragmentation of the sleep cycle. The formation of free radicals or reactive oxygen species (ROS) can emerge and react with nitric oxide (NO) to produce peroxynitrite, thereby diminishing the bioavailability of NO. Hypoxia, the hallmark of OSA, refers to a decline of tissue oxygen saturation and affects several types of cells, playing cell-to-cell communication a vital role in the outcome of this interplay. Red blood cells (RBCs) are considered transporters of oxygen and nutrients to the tissues, and these RBCs are important interorgan communication systems with additional functions, including participation in the control of systemic NO metabolism, redox regulation, blood rheology, and viscosity. RBCs have been shown to induce endothelial dysfunction and increase cardiac injury. The mechanistic links between changes of RBC functional properties and cardiovascular are largely unknown. Extracellular vesicles (EVs) are secreted by most cell types and released in biological fluids both under physiological and pathological conditions. EVs are involved in intercellular communication by transferring complex cargoes including proteins, lipids, and nucleic acids from donor cells to recipient cells. Advancing our knowledge about mechanisms of RBC-EVs formation and their pathophysiological relevance may help to shed light on circulating EVs and to translate their application to clinical practice. We will focus on the potential use of RBC-EVs as valuable diagnostic and prognostic biomarkers and state-specific cargoes, and possibilities as therapeutic vehicles for drug and gene delivery. The use of RBC-EVs as a precision medicine for the diagnosis and treatment of the patient with sleep disorder will improve the prognosis and the quality of life in patients with cardiovascular disease (CVD).

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Potential of extracellular vesicles in the pathogenesis, diagnosis and therapy for parasitic diseases

Pinheiro,

Torrecilhas,

Souza

et al. 2024

J of Extracellular Vesicle

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Parasitic diseases have a significant impact on human and animal health, representing a major hazard to the public and causing economic and health damage worldwide. Extracellular vesicles (EVs) have long been recognized as diagnostic and therapeutic tools but are now also known to be implicated in the natural history of parasitic diseases and host immune response modulation. Studies have shown that EVs play a role in parasitic disease development by interacting with parasites and communicating with other types of cells. This review highlights the most recent research on EVs and their role in several aspects of parasite‐host interactions in five key parasitic diseases: Chagas disease, malaria, toxoplasmosis, leishmaniasis and helminthiases. We also discuss the potential use of EVs as diagnostic tools or treatment options for these infectious diseases.

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Extracellular vesicles derived from Plasmodium-infected and non-infected red blood cells as targeted drug delivery vehicles

Cited by 30 publications

References 75 publications

Extracellular vesicles in malaria: an agglomeration of two decades of research

Extracellular vesicles in malaria: an agglomeration of two decades of research

The Mystery of Red Blood Cells Extracellular Vesicles in Sleep Apnea with Metabolic Dysfunction

Potential of extracellular vesicles in the pathogenesis, diagnosis and therapy for parasitic diseases

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