Extracellular Vesicles Carry HIV Env and Facilitate Hiv Infection of Human Lymphoid Tissue

Arakelyan, Anush; Fitzgerald, Wendy; Zicari, Sonia; Vanpouille, Christophe; Margolis, Leonid

doi:10.1038/s41598-017-01739-8

Cited by 74 publications

(64 citation statements)

References 38 publications

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“…E-mail: sjp10@ st-andrews.ac.uk. 2 The abbreviations used are: EV, extracellular vesicle; MVB, multivesicular bodies; NTA, nanoparticle tracking analysis; MAE, mild acid elution.…”

Section: Resultsmentioning

confidence: 99%

“…Extracellular vesicles (EVs), 2 including those known as exosomes, are released by many types of cells, and have been ascribed numerous functions in cellular communication, including the propagation of niche sites for tumor metastases (1), facilitating viral infection (2), and in angiogenesis (3), among many others. EVs are thought to originate from multivesicular bodies and are secreted following fusion of multivesicular bodies (MVB) with the plasma membrane (4,5).…”

mentioning

confidence: 99%

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The major histocompatibility complex class I immunopeptidome of extracellular vesicles

Synowsky

Shirran

Cooke

et al. 2017

Journal of Biological Chemistry

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Extracellular vesicles (EVs) are released by most cell types and have been associated with multiple immunomodulatory functions. MHC class I molecules have crucial roles in antigen presentation and in eliciting immune responses and are known to be incorporated into EVs. However, the MHC class I immunopeptidome of EVs has not been established. Here, using a small-scale immunoisolation of the antigen serotypes HLA-A*02:01 and HLA-B*27:05 expressed on the Epstein-Barr virus-transformed B cell line Jesthom and MS of the eluted peptides from both cells and EVs, we identified 516 peptides that bind either HLA-A*02:01 or HLA-B*27:05. Of importance, the predicted serotype-binding affinities and peptide-anchor motifs did not significantly differ between the peptide pools isolated from cells or EVs, indicating that during EV biogenesis, no obvious editing of the MHC class I immunopeptidome occurs. These results, for the first time, establish EVs as a source of MHC class I peptides that can be used for the study of the immunopeptidome and in the discovery of potential neoantigens for immunotherapies.

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“…E-mail: sjp10@ st-andrews.ac.uk. 2 The abbreviations used are: EV, extracellular vesicle; MVB, multivesicular bodies; NTA, nanoparticle tracking analysis; MAE, mild acid elution.…”

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

The major histocompatibility complex class I immunopeptidome of extracellular vesicles

Synowsky

Shirran

Cooke

et al. 2017

Journal of Biological Chemistry

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“…Interestingly, reducing the expression of VSP4 and ALIX affects the release of eHAVs, but not other ESCRT components (Feng et al, 2013). Moreover, the human immunodeficiency virus (HIV) and herpes simplex virus 1 (HSV-1) exploit extracellular vesicles to enhance their spread into uninfected cells (Arakelyan et al, 2017). Conceptually, an emerging paradigm is that viruses may hijack the extracellular vesicle machinery to invade mammalian cells.…”

Section: Viral Invasionmentioning

confidence: 99%

Message in a vesicle – trans-kingdom intercommunication at the vector–host interface

Chávez

O’Neal

Santambrogio

et al. 2019

Journal of Cell Science

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Vector-borne diseases cause over 700,000 deaths annually and represent 17% of all infectious illnesses worldwide. This public health menace highlights the importance of understanding how arthropod vectors, microbes and their mammalian hosts interact. Currently, an emphasis of the scientific enterprise is at the vector-host interface where human pathogens are acquired and transmitted. At this spatial junction, arthropod effector molecules are secreted, enabling microbial pathogenesis and disease. Extracellular vesicles manipulate signaling networks by carrying proteins, lipids, carbohydrates and regulatory nucleic acids. Therefore, they are well positioned to aid in cell-to-cell communication and mediate molecular interactions. This Review briefly discusses exosome and microvesicle biogenesis, their cargo, and the role that nanovesicles play during pathogen spread, host colonization and disease pathogenesis. We then focus on the role of extracellular vesicles in dictating microbial pathogenesis and host immunity during transmission of vector-borne pathogens.

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“…In addition to stimulating EV production by host cells, HIV can utilize exosomes to enter immune cells, notably because exosomes released by HIV‐1‐infected cells contain gp120 (Env), a viral protein that mediates virus attachment and fusion to target cells …”

Section: Examples Of Infectious Diseases With Ev Involvement In Theirmentioning

confidence: 99%

Extracellular vesicles as mediators of immunopathology in infectious diseases

Hosseini‐Beheshti

Grau

2018

Immunol Cell Biol

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In the last decades, extracellular vesicles have emerged as important elements in cell-cell communication and as key players in disease pathogenesis via transmission of their cargo between different cells. Various works have described different subpopulations of these membrane structures, based on their cell of origin, biogenesis, size, biophysical properties and cargo. In addition to their pathophysiological role in the development and progression of different diseases including infectious diseases, neurodegenerative disorders and cancer, extracellular vesicles are now recognized for their potential as novel therapeutic targets and intelligent drug delivery system. Here, we have reviewed the most recent data on different subtypes of extracellular vesicles, focusing on microvesicles and exosomes and their subpopulations, their involvement in immune-mediated pathogenesis of various infectious diseases and their role as potential therapeutic targets.

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Extracellular Vesicles Carry HIV Env and Facilitate Hiv Infection of Human Lymphoid Tissue

Cited by 74 publications

References 38 publications

The major histocompatibility complex class I immunopeptidome of extracellular vesicles

The major histocompatibility complex class I immunopeptidome of extracellular vesicles

Message in a vesicle – trans-kingdom intercommunication at the vector–host interface

Extracellular vesicles as mediators of immunopathology in infectious diseases

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