Extracellular vesicles as a source for non-invasive biomarkers in bladder cancer progression

Andreu, Zoraida; Oshiro, Renan Otta; Redruello, Alberto; López-Martín, Sara; Gutiérrez-Vázquez, Cristina; Morato, Esperanza; Marina, Ana Isabel; Gómez, C. Olivier; Yáñez-Mó, Marı́a

doi:10.1016/j.ejps.2016.10.008

Cited by 84 publications

(61 citation statements)

References 69 publications

(72 reference statements)

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“…ApoB is another BCEV protein with a functional link to the inflammation process [ 48 ]. ApoB is another BCEV protein with a functional link to the inflammation process [ 49 ]. Andreu et al compared the urinary EV protein profiles of BC patients versus healthy non-smokers and found that ApoB expression was significantly increased in BC patient-derived EVs.…”

Section: Regulation Of Immune Responses By Bcevsmentioning

confidence: 99%

Extracellular Vesicles in Bladder Cancer: Biomarkers and Beyond

Liu

Ortiz-Bonilla

Lee

2018

IJMS

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Tumor-derived extracellular vesicles (TEVs) are membrane-bound, nanosized vesicles released by cancer cells and taken up by cells in the tumor microenvironment to modulate the molecular makeup and behavior of recipient cells. In this report, we summarize the pivotal roles of TEVs involved in bladder cancer (BC) development, progression and treatment resistance through transferring their bioactive cargos, including proteins and nucleic acids. We also report on the molecular profiling of TEV cargos derived from urine and blood of BC patients as non-invasive disease biomarkers. The current hurdles in EV research and plausible solutions are discussed.

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Section: Regulation Of Immune Responses By Bcevsmentioning

confidence: 99%

Extracellular Vesicles in Bladder Cancer: Biomarkers and Beyond

Liu

Ortiz-Bonilla

Lee

2018

IJMS

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“…Being naturally present in every living organism, endogenous EVs at physiological concentrations are also intrinsically characterized by low toxicity, high stability, biocompatibility, and permeability to biological barriers . Hence, increasing research efforts have been devoted to evaluate the outstanding potential of EVs as therapeutic agents, diagnostic tools for liquid biopsy, and delivery systems for drugs, cosmetics, or nutraceuticals …”

Section: Introductionmentioning

confidence: 99%

Scalable Production and Isolation of Extracellular Vesicles: Available Sources and Lessons from Current Industrial Bioprocesses

Paganini

Palmiero

Pócsfalvi

et al. 2019

Biotechnology Journal

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Potential applications of extracellular vesicles (EVs) are attracting increasing interest in the fields of medicine, cosmetics, and nutrition. However, the manufacturing of EVs is currently characterized by low yields. This limitation severely hampers progress in research at the laboratory and clinical scales, as well as the realization of successful and cost-effective EV-based products. Moreover, the high level of heterogeneity of EVs further complicates reproducible manufacturing on a large scale. In this review, possible directions toward the scalable production of EVs are discussed. In particular, two strategies are considered: i) the optimization of upstream unit operations and ii) the exploitation of well-established and mature technologies already in use in other industrial bioprocesses.

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“…EVs, analyzed by MS based proteomics, demonstrated specific protein and miRNAs pattern in BC patients ( 116 ). By using a microarray platform and RT-PCR analysis, miR-375, and miR146a have been found to specifically identify high-grade and low-grade BC, respectively ( 100 ). The application of nanowires anchored into a microfluidic substrate will enable the efficiency of EV collection, thus permitting to identify EV harboring miRNAs ( 117 ).…”

Section: Extracellular Vesicles (Evs) and Exosomesmentioning

confidence: 99%

Urinary Markers in Bladder Cancer: An Update

et al. 2018

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Bladder cancer (BC) is ones of the most common cancer worldwide. It is classified in muscle invasive (MIBC) and muscle non-invasive (NMIBC) BC. NMIBCs frequently recur and progress to MIBCs with a reduced survival rate and frequent distant metastasis. BC detection require unpleasant and expensive cystoscopy and biopsy, which are often accompanied by several adverse effects. Thus, there is an urgent need to develop novel diagnostic methods for initial detection and surveillance in both MIBCs and NMIBCs. Multiple urine-based tests approved by FDA for BC detection and surveillance are commercially available. However, at present, sensitivity, specificity and diagnostic accuracy of these urine-based assays are still suboptimal and, in the attend to improve them, novel molecular markers as well as multiple-assays must to be translated in clinic. Now there are growing evidence toward the use of minimally invasive “liquid biopsy” to identify biomarkers in urologic malignancy. DNA- and RNA-based markers in body fluids such as blood and urine are promising potential markers in diagnostic, prognostic, predictive and monitoring urological malignancies. Thus, circulating cell-free DNA, DNA methylation and mutations, circulating tumor cells, miRNA, IncRNA and mRNAs, cell-free proteins and peptides, and exosomes have been assessed in urine specimens. However, proteomic and genomic data must to be validated in well-designed multicenter clinical studies, before to be employed in clinic oncology.

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Extracellular vesicles as a source for non-invasive biomarkers in bladder cancer progression

Cited by 84 publications

References 69 publications

Extracellular Vesicles in Bladder Cancer: Biomarkers and Beyond

Extracellular Vesicles in Bladder Cancer: Biomarkers and Beyond

Scalable Production and Isolation of Extracellular Vesicles: Available Sources and Lessons from Current Industrial Bioprocesses

Urinary Markers in Bladder Cancer: An Update

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