Extracellular vesicles are key intercellular mediators in the development of immune dysfunction to allergens in the airways

Shin, T.-S.; Kim, J H; Ys, Kim; Jeon, Seong Gyu; Zhu, Zhaohui; Gho, Yong Song; Kim, Eui Chong

doi:10.1111/j.1398-9995.2010.02359.x

Cited by 25 publications

(31 citation statements)

References 30 publications

(43 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Previous research on BAL MVs has suggested that this MV population has a role in immunity and acts as a conveyor of signals in allergen and infectious immune response 36,37 . Supporting their role in immunity was the finding that BAL MVs are shown to express CD36, which has been implicated in bacterial recognition 38 .…”

Section: Discussionmentioning

confidence: 99%

Bronchoalveolar Lavage Microvesicles Protect Burn-Injured Mice from Pulmonary Infection

Rice

Pugh

Xia

et al. 2017

Journal of the American College of Surgeons

View full text Add to dashboard Cite

Background Pseudomonas aeruginosa (PA) is a major cause of morbidity and mortality among burn patients, despite antibiotic therapy. There is a need to identify innate immune defenses that prevent PA infection in injured adults in an effort to find therapeutic alternatives to antibiotics. Here, we tested our hypothesis that Microvesicles (MVs) in bronchoalveolar (BAL) fluid have a role in the immunity of the lung in response to pathogens. Study Design MVs were isolated from murine BAL fluid, quantified using nanoparticle tracking analysis, and injected into burn injured mice prior to PA infection. Survival was assessed and BAL bacterial loads enumerated. Neutrophil number and IL-6 activity were determined. Lungs were harvested and sphingosine (SPH) content analyzed via immunohistochemistry. Antimicrobial effects of MV and SPH enriched MVs were assessed in an in vitro assay. Results Burn injured mice have reduced BAL MV number and SPH content as compared to sham. When BAL MVs from healthy mice are administered to injured mice, survival and bacterial clearance are robustly improved. We further observed that intranasal administration of MVs restores SPH levels after burn injury, MVs directly kill bacteria, and this bacterial killing is increased when the MVs supplemented with SPH. Conclusion Using a pre-clinical model, BAL MVs are reduced after scald injury and BAL MV restoration to injured mice improves survival and bacterial clearance. The antimicrobial mechanisms leading to improved survival includes the quantity and SPH content of BAL MVs.

show abstract

Section: Discussionmentioning

confidence: 99%

Bronchoalveolar Lavage Microvesicles Protect Burn-Injured Mice from Pulmonary Infection

Rice

Pugh

Xia

et al. 2017

Journal of the American College of Surgeons

View full text Add to dashboard Cite

show abstract

“…BAL fluid was collected and neutrophils were counted as previously described [41]. Lungs were harvested after whole body perfusion.…”

Section: Methodsmentioning

confidence: 99%

Outer Membrane Vesicles Derived from Escherichia coli Up-Regulate Expression of Endothelial Cell Adhesion Molecules In Vitro and In Vivo

et al. 2013

View full text Add to dashboard Cite

Escherichia coli, as one of the gut microbiota, can evoke severe inflammatory diseases including peritonitis and sepsis. Gram-negative bacteria including E. coli constitutively release nano-sized outer membrane vesicles (OMVs). Although E. coli OMVs can induce the inflammatory responses without live bacteria, the effect of E. coli OMVs in vivo on endothelial cell function has not been previously elucidated. In this study, we show that bacteria-free OMVs increased the expression of endothelial intercellular adhesion molecule-1 (ICAM-1), E-selectin and vascular cell adhesion molecule-1, and enhanced the leukocyte binding on human microvascular endothelial cells in vitro. Inhibition of NF-κB and TLR4 reduced the expression of cell adhesion molecules in vitro. OMVs given intraperitoneally to the mice induced ICAM-1 expression and neutrophil sequestration in the lung endothelium, and the effects were reduced in ICAM-1-/- and TLR4-/- mice. When compared to free lipopolysaccharide, OMVs were more potent in inducing both ICAM-1 expression as well as leukocyte adhesion in vitro, and ICAM-1 expression and neutrophil sequestration in the lungs in vivo. This study shows that OMVs potently up-regulate functional cell adhesion molecules via NF-κB- and TLR4-dependent pathways, and that OMVs are more potent than free lipopolysaccharide.

show abstract

“…Interestingly, in BALF from patients with sarcoidosis, a systemic chronic inflammatory disease predominantly affecting the lung, phenotypically and functionally different vesicles are present compared to healthy individuals [30]. Also intranasal administration of LPS, causing increased infiltration of inflammatory cells and Th1 and Th17 dominated lung inflammation, resulted in quantitative and qualitative changes in the vesicle population isolated from BALF [136].…”

Section: The Role Of Immune Cell-derived Vesicles In Allergic Inflammmentioning

confidence: 97%

Immune Cell-derived Vesicles: Modulators and Mediators of Inflammation

Hoen¹,

Wauben

2012

CPD

View full text Add to dashboard Cite

Most cell types can release vesicles. Cell-derived vesicles are increasingly recognized as an evolutionary wide-spread mechanism of intercellular communication. The paracrine and long range activity of vesicles and their regulated cargo-composition endows these vesicles with regulatory properties beyond that of the parental cell. The release and biogenesis of cell-derived vesicles is a dynamic and tightly controlled process. In the past years it has become clear that these vesicles exert a plethora of biological effects. This has sparked the intense interest in these vesicles in relation to (patho)physiological processes. This review focuses on the role of cell-derived vesicles in inflammation, with emphasis on the immune modulating capacity of immune cell-derived vesicles. The biological activity of different leukocyte-derived vesicles is compared, and potential explanations for the strong biological effects exhibited by vesicles are provided. The role of cell-derived vesicles in inflammatory processes is discussed by speculating how these vesicles can contribute to allergic inflammation.

show abstract

Extracellular vesicles are key intercellular mediators in the development of immune dysfunction to allergens in the airways

Cited by 25 publications

References 30 publications

Bronchoalveolar Lavage Microvesicles Protect Burn-Injured Mice from Pulmonary Infection

Bronchoalveolar Lavage Microvesicles Protect Burn-Injured Mice from Pulmonary Infection

Outer Membrane Vesicles Derived from Escherichia coli Up-Regulate Expression of Endothelial Cell Adhesion Molecules In Vitro and In Vivo

Immune Cell-derived Vesicles: Modulators and Mediators of Inflammation

Contact Info

Product

Resources

About