Extracellular Vesicles and the Oviduct Function

Harris, Emily A.; Stephens, Kalli K.; Winuthayanon, Wipawee

doi:10.3390/ijms21218280

Cited by 44 publications

(38 citation statements)

References 92 publications

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“…In the uterus, stromal‐derived IGF1 stimulates epithelial cell proliferation through a paracrine mechanism 11 . It was previously shown that secreted factors present in the extracellular vesicles can diffuse through the zona pellucida and bind to the plasma membrane of bovine preimplantation embryos 73 . Therefore, it is possible that in the mouse oviduct, secreted IGF1 can be diffused in a similar fashion as in the uterus through epithelial cells and promote embryo development in the oviductal lumen.…”

Section: Discussionmentioning

confidence: 99%

Cell‐type specific analysis of physiological action of estrogen in mouse oviducts

et al. 2021

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One of the endogenous estrogens, 17β-estradiol (E 2 ) is a female steroid hormone secreted from the ovary. It is well established that E 2 causes biochemical and histological changes in the uterus. However, it is not completely understood how E 2 regulates the oviductal environment in vivo. In this study, we assessed the effect of E 2 on each oviductal cell type, using an ovariectomized-hormone-replacement mouse model, single-cell RNA-sequencing (scRNA-seq), in situ hybridization, and celltype-specific deletion in mice. We found that each cell type in the oviduct responded to E 2 distinctively, especially ciliated and secretory epithelial cells. The treatment of exogenous E 2 did not drastically alter the transcriptomic profile from that of endogenous E 2 produced during estrus. Moreover, we have identified and validated genes of interest in our datasets that may be used as cell-and region-specific markers in the oviduct. Insulin-like growth factor 1 (Igf1) was characterized as an E 2 -target gene in the mouse oviduct and was also expressed in human fallopian tubes. Deletion of Igf1 in progesterone receptor (Pgr)-expressing cells resulted in female subfertility, partially due to an embryo developmental defect and embryo retention within the oviduct. In summary, we have shown that oviductal cell types, including epithelial, stromal, and muscle cells, are differentially regulated by E 2 and support gene expression changes, such as growth factors that are required for normal embryo development and transport in mouse models. Furthermore, we have identified cell-specific and region-specific gene markers for targeted studies and functional analysis in vivo. K E Y W O R D Sembryo development, embryo transport, estrogen, insulin-like growth factor 1, oviduct, scRNA-seq 2 of 19 | MCGLADE Et AL.

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Section: Discussionmentioning

confidence: 99%

Cell‐type specific analysis of physiological action of estrogen in mouse oviducts

et al. 2021

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“…Many efforts have been made to alter the fertilization conditions to improve monospermy rates. These efforts have included altering the concentration of sperm while varying the duration of exposure between the sperm and oocytes, adding follicular fluid [33] or substances found in oviductal fluid, such as deleted in malignant brain tumors 1 (DMBT1) [34], oviductal glycoprotein 1 (OVGP1) [35], secreted phosphoprotein 1 (SPP1) [36], or plasminogen [37,38], as well as trying to recreate the oviductal environment by adding oviductal fluid [39,40] or oviductal extracellular vesicles [41], among other things [42].…”

Section: In Vitro Fertilizationmentioning

confidence: 99%

Challenges and Considerations during In Vitro Production of Porcine Embryos

Chen

Redel²,

Kerns

et al. 2021

Cells

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Genetically modified pigs have become valuable tools for generating advances in animal agriculture and human medicine. Importantly, in vitro production and manipulation of embryos is an essential step in the process of creating porcine models. As the in vitro environment is still suboptimal, it is imperative to examine the porcine embryo culture system from several angles to identify methods for improvement. Understanding metabolic characteristics of porcine embryos and considering comparisons with other mammalian species is useful for optimizing culture media formulations. Furthermore, stressors arising from the environment and maternal or paternal factors must be taken into consideration to produce healthy embryos in vitro. In this review, we progress stepwise through in vitro oocyte maturation, fertilization, and embryo culture in pigs to assess the status of current culture systems and address points where improvements can be made.

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“…Also, when it comes to the proper communication and regulation between gametes/embryos and the fallopian tube, extracellular vesicles also paly important role in the multi-steps process. For example, proteins including endothelial nitric oxide synthase (eNOS), PMCA1 and PMCA4 can be delivered to sperm by extracellular vesicles via a fusogenic mechanism, and contributing to the sperm viability (37)(38)(39).…”

Section: Female Fertility Healthy Physiology and Extracellular Vesiclesmentioning

confidence: 99%

The Emerging Roles and Therapeutic Potential of Extracellular Vesicles in Infertility

Zhou

et al. 2021

Front. Endocrinol.

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Infertility is becoming much more common and affects more couples. The past years witnessed the rapid development of the diagnosis and treatment upon infertility, which give numerous coupled more opportunities become parents. Extracellular vesicles are known as nano-sized membrane vesicles to play a major role in intracellular communication. In recent years, several basic and clinical studies have tried to investigate the correlation between the reproductive health/disorder and extracellular vesicles. However, the mechanism is still unclear. In this review, we reviewed the relationship between reproductive physiology and extracellular vesicles, and then collectively focused on the recent findings on the relationship between extracellular and infertility, and its consequent influence on the novel insight regarding the therapeutic strategies for infertility in the future clinical practice.

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Extracellular Vesicles and the Oviduct Function

Cited by 44 publications

References 92 publications

Cell‐type specific analysis of physiological action of estrogen in mouse oviducts

Cell‐type specific analysis of physiological action of estrogen in mouse oviducts

Challenges and Considerations during In Vitro Production of Porcine Embryos

The Emerging Roles and Therapeutic Potential of Extracellular Vesicles in Infertility

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