Extracellular Vesicle Mediated Vascular Pathology in Glioblastoma

Spinelli, Cristiana; Tawil, Nadim; Adnani, Lata; Rak, Janusz; Choi, Dong-Sic

doi:10.1007/978-3-030-67171-6_10

Cited by 6 publications

(7 citation statements)

References 147 publications

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“…Cell lysates for Western blot were obtained by incubating HEK293 (for uEV Western blot) and PC3 or DU145 (for cEV Western blot) cell pellets at a concentration of 2 × 10 (Hoshino et al., 2020) cells in 25 ul of lysis buffer (50 mM Tris, pH 7.5, 0.15 M NaCl, 1% Triton X‐100) with 2% complete protease inhibitor (Roche) for 20 min on ice, followed by an 18,516× g centrifugation for 15 min at 4 °C to recover the supernatant. Both cell lysates and EV pellets (10 (Spinelli et al., 2021) particles) were mixed with Laemmli sample buffer (BioRad), without reducing agent. After boiling for 5 min at 95 °C, samples were loaded on a 4–15% Miniprotean TGX stain‐free gels (BioRad).…”

Section: Methodsmentioning

confidence: 99%

“…Since EVs work as a safe way to transport biological information through the whole body, they are now recognized as an important source of biomarkers in clinics but also as a mechanism of cell‐cell communication within tumour cells and their distant environment (Becker et al., 2016; Tkach & Théry, 2016). Multiple studies in different cancer types have demonstrated that EVs play an important role in cell proliferation, migration, invasion, angiogenesis, epithelial‐to‐mesenchymal transition (EMT), metastasis and immune response (Kalluri & Lebleu, 2020; Pelissier Vatter et al., 2021; Spinelli et al., 2021; Tkach & Théry, 2016). EVs also act as a source of antigenic peptides for the activation of T and B cells (Chaput et al., 2004; Wolfers et al., 2001).…”

Section: Introductionmentioning

confidence: 99%

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Urinary extracellular vesicles contain mature transcriptome enriched in circular and long noncoding RNAs with functional significance in prostate cancer

Almeida

Gabriel

Firlej

et al. 2022

J of Extracellular Vesicle

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Long noncoding (lnc)RNAs modulate gene expression alongside presenting unexpected source of neoantigens. Despite their immense interest, their ability to be transferred and control adjacent cells is unknown. Extracellular Vesicles (EVs) offer a protective environment for nucleic acids, with pro and antitumourigenic functions by controlling the immune response. In contrast to extracellular nonvesicular RNA, few studies have addressed the full RNA content within human fluids' EVs and have compared them with their tissue of origin. Here, we performed Total RNA-Sequencing on six Formalin-Fixed-Paraffin-Embedded (FFPE) prostate cancer (PCa) tumour tissues and their paired urinary (u)EVs to provide the first whole transcriptome comparison from the same patients. UEVs contain simplified transcriptome with intronfree cytoplasmic transcripts and enriched lnc/circular (circ)RNAs, strikingly common to an independent 20 patients' urinary cohort. Our full cellular and EVs transcriptome comparison within three PCa cell lines identified a set of overlapping 14 uEV-circRNAs characterized as essential for prostate cell proliferation in vitro and 28 uEV-lncRNAs belonging to the cancer-related lncRNA census (CLC2). In addition, we found 15 uEV-lncRNAs, predicted to encode 768 high-affinity neoantigens, and for which three of the encoded-ORF produced detectable unmodified peptidesThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Urinary extracellular vesicles contain mature transcriptome enriched in circular and long noncoding RNAs with functional significance in prostate cancer

Almeida

Gabriel

Firlej

et al. 2022

J of Extracellular Vesicle

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“…Exosome is a kind of spherical nano‐sized vesicles comprising phospholipid bilayer membrane with a diameter ranging from 30 nm to 200 nm and a density of 1.13–1.19 g/mL [1] . Exosomes are released by parent cells through the endocytic pathway, carrying important information in regulating physiological and pathological conditions of target cells [2] .…”

Section: Introductionmentioning

confidence: 99%

“…Exosome is a kind of spherical nano-sized vesicles comprising phospholipid bilayer membrane with a diameter ranging from 30 nm to 200 nm and a density of 1.13-1.19 g/mL. [1] Exosomes are released by parent cells through the endocytic pathway, carrying important information in regulating physiological and pathological conditions of target cells. [2] Exosomes can induce endothelial dysfunction, vascular inflammation, coagulation, thrombosis, and calcification via their components of proteins and noncoding RNAs, which may promote the progression of cerebral venous thrombosis.…”

Section: Introductionmentioning

confidence: 99%

Allosteric Probe Recognition Initiated Cascade Transcription Amplification for Sensitive Analysis of Exosomes

Lei

Zhang

et al. 2022

ChemBioChem

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Sensitive and accurate analysis of exosomes is important for many biological processes including as a biomarker for cerebral venous thrombosis (CVT) diagnosis and therapy. Herein, we established a sensitive and specific exosome detection approach based on target recognition initiated cascade signal amplification. In this method, an allosteric probe was designed with a hairpin structure for specific recognition of the exosome followed by signal amplification. After the cascade signal amplification process, spinach RNA sequences bind to DFHBI ((Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-1,2-dimethyl-1H-imidazol-5(4H)-one) to produce enhanced fluorescence signal (approximate 2000 fold than that of inactive DFHBI). Compared with former proposed exosome detection techniques, this method exhibited a comparable detection range, but with an easy-to-design toolbox. Therefore, we believe that the proposed approach holds great potential for exosome based early diagnosis and prognosis of disease.

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Section: Introductionmentioning

confidence: 99%

Prostate cancer urinary Extracellular Vesicles are enriched in cytoplasmic intron-free mRNAs and circular/long noncoding RNAs with functional significance

Almeida¹,

Gabriel²,

Firlej³

et al. 2021

Preprint

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Long noncoding (lnc)RNAs modulate gene expression alongside presenting unexpected source of neoantigens. Despite their immense interest, their ability to be transferred and control adjacent cells is unknown. Extracellular Vesicles (EVs) offer a protective environment for nucleic acids, with pro and anti-tumorigenic functions by controlling the immune response. In contrast to extracellular non-vesicular RNA, few studies have addressed the full RNA content within human fluids’ EVs and none have compared them with their tissue of origin. Here, we performed Total RNA-Sequencing on 6 Formaldehyde-Fixed-Parafilm-Embedded (FFPE) prostate cancer (PCa) tumor tissues and their paired urinary (u)EVs to provide the first whole transcriptome comparison from the same patients. UEVs contain simplified transcriptome with intron-free cytoplasmic transcripts and specific lnc/circular (circ)RNAs, strikingly common to all patients. Our full cellular and EVs transcriptome comparison within 3 common PCa cell lines identified a set of overlapping 14 uEV-circRNAs characterized as essential for prostate cell proliferation in vitro and 15 uEV-lncRNAs that we predicted to encode 768 high-affinity neoantigens. Our dual analysis of EVs-lnc/circRNAs both in urines’ and in vitro’s EVs provides a fundamental resource for future uEV-lnc/circRNAs phenotypic characterization involved in PCa.

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Extracellular Vesicle Mediated Vascular Pathology in Glioblastoma

Cited by 6 publications

References 147 publications

Urinary extracellular vesicles contain mature transcriptome enriched in circular and long noncoding RNAs with functional significance in prostate cancer

Urinary extracellular vesicles contain mature transcriptome enriched in circular and long noncoding RNAs with functional significance in prostate cancer

Allosteric Probe Recognition Initiated Cascade Transcription Amplification for Sensitive Analysis of Exosomes

Prostate cancer urinary Extracellular Vesicles are enriched in cytoplasmic intron-free mRNAs and circular/long noncoding RNAs with functional significance

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