Extracellular tRNAs and tRNA-derived fragments

Tosar, Juan Pablo; Cayota, Alfonso

doi:10.1080/15476286.2020.1729584

Cited by 57 publications

(68 citation statements)

References 175 publications

(313 reference statements)

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“…The transcriptomics of EVs, including signature of EV-derived microRNAs and RNA-derived fragments, is disputed as a promising source of biomarkers in liquid biopsies (142). Future studies that could clearly explain the potency of EVs as biomarkers for personalized care of HF are required.…”

Section: Future Directions and Challenges In Ev Researchmentioning

confidence: 99%

Extracellular Endothelial Cell-Derived Vesicles: Emerging Role in Cardiac and Vascular Remodeling in Heart Failure

Berezin

2020

Front. Cardiovasc. Med.

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Extracellular vesicles play a pivotal role in numerous physiological (immune response, cell-to-cell cooperation, angiogenesis) and pathological (reparation, inflammation, thrombosis/coagulation, atherosclerosis, endothelial dysfunction) processes. The development of heart failure is strongly associated with endothelial dysfunction, microvascular inflammation, alteration in tissue repair, and cardiac and vascular remodeling. It has been postulated that activated endothelial cell-derived vesicles are not just transfer forms of several active molecules (such as regulatory peptides, coagulation factors, growth factors, active molecules, hormones that are embedded onto angiogenesis, tissue reparation, proliferation, and even prevention from ischemia/hypoxia), but are instead involved in direct myocardial and vascular damage due to regulation of epigenetic responses of the tissue. These responses are controlled by several factors, such as micro-RNAs, that are transferred inside extracellular vesicles from mother cells to acceptor cells and are transductors of epigenetic signals. Finally, it is not a uniform opinion whether different phenotypes of heart failure are the result of altered cardiac and vascular reparation due to certain epigenetic responses, which are yielded by co-morbidities, such as diabetes mellitus and obesity. The aim of the review is to summarize knowledge regarding the role of various types of extracellular endothelial cell-derived vesicles in the regulation of cardiac and vascular remodeling in heart failure.

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Section: Future Directions and Challenges In Ev Researchmentioning

confidence: 99%

Extracellular Endothelial Cell-Derived Vesicles: Emerging Role in Cardiac and Vascular Remodeling in Heart Failure

Berezin

2020

Front. Cardiovasc. Med.

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“…Non-coding RNAs (ncRNAs) can be released to circulate in the peripheral blood. Circulating levels of tRFs could be useful as biomarkers of some diseases and surrogate measures of disease progression [13,14], indicating their potential use in diagnosis and assessment of I/R in pediatric intussusception. In our previous studies, we found I/R in both our animal models and our pediatric intussusception patients.…”

Section: Discussionmentioning

confidence: 99%

“…Levels of tRFs can uctuate in response to stimuli. Circulating levels of tRFs might be useful as the surrogate measurements of disease progression [13,14].…”

Section: Introductionmentioning

confidence: 99%

Serum tRNA-Derived Fragments as Potential Biomarkers in Children with Acute Intussusception

Chen

Zhao

Guo

2020

Preprint

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Background: The aim of the present study was to investigate whether transfer ribonucleic acid (tRNA)–derived fragments (tRFs) can serve as candidate biomarkers for pediatric intussusception.Methods: Using high-throughput sequencing technology, we identified differentially expressed tRFs, and ultimately selected three tRFs to establish a signature as a predictive biomarker of pediatric intussusception. Selection of these three upregulated genes was veriﬁed using quantitative reverse-transcription polymerase chain reaction (qRT-PCR). We conducted receiver operator characteristic (ROC) curve analysis to evaluate the predictive accuracy of the selected genes for pediatric intussusception.Results: We detected 732 tRFs and tRNA-derived stress-induced RNA (tiRNAs), 1705 micro-RNAs (miRNAs), 52 differentially expressed miRNAs, and 34 differentially expressed tRFs and tiRNAs between patients and controls. Compared with controls, we found 33 upregulated miRNAs, 24 upregulated tRFs and tiRNAs, 19 downregulated miRNAs, and 10 downregulated tRFs and tiRNAs in children with intussusception. Using qPCR, the expression trends of tRF-Leu-TAA-006, tRF-Gln-TTG-033 and tRF-Lys-TTT-028 were consistent with the sequencing results. AUCs of tRF-Leu-TAA-006, tRF-Gln-TTG-033 and tRF-Lys-TTT-028 were 0.984, 0.970 and 0.837, respectively.Conclusion: Circulating tRF-Leu-TAA-006, tRF-Gln-TTG-033 and tRF-Lys-TTT-028 expression might be a novel potential biomarker for diagnosis of pediatric intussusception.

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“…When combined with cytochrome C, tRFs inhibit the formation of apoptotic bodies. Finally, studies have shown that tRF exists in hematopoietic cells, lymphocytes, and the blood circulation system, suggesting that these fragments may play a role in immune response [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

A Comprehensive Expression Profile of tRNA-Derived Fragments in Papillary Thyroid Cancer

Shan

Wang

Zhu

2020

JSO

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Objective: In recent years, the incidence of thyroid cancer has been increasing. Papillary thyroid cancer (PTC) is the most common type of malignant thyroid tumor, accounting for approximately 85% of thyroid cancer cases. Although the genetic background of PTC has been studied extensively, relatively little is known about the role of small non-coding RNA (sncRNA) in this disease. tRNA-derived fragments (tRFs) represent a newly discovered class of sncRNAs that exist in many species and play a role in many biological processes. Methods: In this study, we used RNA sequencing to analyse the expression of tRFs in fresh frozen specimens from PTC tissues and normal tissues adjacent to the tumors. Through this analysis, we identified 49 unique tRFs and transfer RNA halves and then performed quantitative PCR to determine the expression levels of these molecules and to make bioinformatic predictions. Conclusion: In this report, we provide a comprehensive catalog of tRFs in PTC and assess the abnormal expression of these fragments. These preliminary findings can be used as the basis for further research regarding the functional role of tRFs in patients with PTC.

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Extracellular tRNAs and tRNA-derived fragments

Cited by 57 publications

References 175 publications

Extracellular Endothelial Cell-Derived Vesicles: Emerging Role in Cardiac and Vascular Remodeling in Heart Failure

Extracellular Endothelial Cell-Derived Vesicles: Emerging Role in Cardiac and Vascular Remodeling in Heart Failure

Serum tRNA-Derived Fragments as Potential Biomarkers in Children with Acute Intussusception

A Comprehensive Expression Profile of tRNA-Derived Fragments in Papillary Thyroid Cancer

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