Extracellular Simian Virus 40 Induces an ERK/MAP Kinase-independent Signalling Pathway that Activates Primary Response Genes and Promotes Virus Entry

Dangoria, Nandita S.; Breau, Walter C.; Anderson, Howard A.; Cishek, Dawn M.; Norkin, Leonard C.

doi:10.1099/0022-1317-77-9-2173

Cited by 71 publications

(70 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Relatively large macromolecules enter cells through either macropinosomes or phagosomes, whereas small molecules (as described earlier) typically enter by clathrin-, caveolarmediated processes, or micropinocytosis. Macropinocytosis is inhibited by amiloride, which blocks the Na + /H + exchanger and inhibits ruffling 85,86 (Figure 2). Macropinocytosis is also dependent on PI3K and Rho GTPase activities, which regulate actin reorganization.…”

Section: Macropinocytosismentioning

confidence: 99%

Intracellular trafficking of nonviral vectors

2005

View full text Add to dashboard Cite

Section: Macropinocytosismentioning

confidence: 99%

Intracellular trafficking of nonviral vectors

2005

View full text Add to dashboard Cite

“…The protein tyrosine kinase inhibitor, genistein, prevents caveolar endocytosis of SV40 34 and macromolecules, 35 but did not affect perinuclear accumulation of PB although nuclear uptake was inhibited nearly 50%. It is possible that signal modulation through phosphorylation is a partial requirement for nuclear entry.…”

Section: Penton Base Trafficking a Rentsendorj Et Almentioning

confidence: 99%

“…32,33 Perinuclear accumulation was dramatically inhibited by nearly 90% and nuclear accumulation was nearly completely abolished compared to PB uptake without MBCD (Figure 8). To better delineate uptake routes, we treated cells with the protein tyrosine kinase inhibitor, genistein, which prevents caveolae-mediated uptake of SV40 34 and macromolecules. 35 Genistein at 0.1 mM affected PB uptake and perinuclear accumulation a little, whereas nuclear accumulation was inhibited by nearly 50% compared to uptake without the drug ( Figure 8).…”

Section: Effect Of Traffic Inhibitorsmentioning

confidence: 99%

Typical and atypical trafficking pathways of Ad5 penton base recombinant protein: implications for gene transfer

et al. 2006

View full text Add to dashboard Cite

The adenovirus (Ad) penton base protein facilitates viral infection by binding cell surface integrins, triggering receptormediated endocytosis and mediating endosomal penetration. Given these multiple functions, recombinant penton base proteins have been utilized as non-viral vehicles for gene transfer by our lab and others. Although we have previously demonstrated that penton base-derived vectors undergo integrin-specific binding and cell entry, less than desirable levels of gene expression have led us to re-evaluate the recombinant penton base as an agent for gene delivery. To do so, we have examined here the intracellular trafficking of an Ad serotype 5 (Ad5) recombinant penton base protein (PB). Here, we not only observed that PB utilizes a similar, typical trafficking pathway of whole Ad, but also found that PB entered HeLa cells through pathways not yet identified as contributing to cell entry by the whole virus. We show by high-resolution confocal microscopy and biochemical methods that binding to a v -integrins is a requirement for cell entry, but that early internalization stages did not substantially pass through clathrin-positive and early endosomal compartments. Moreover, a subpopulation of internalized protein localized with caveolin-positive compartments and Golgi markers, suggesting that a certain percentage of proteins pass through nonclathrin-mediated pathways. Similar to the virus, trafficking toward the nucleus was affected by disruption of microtubules and dynein. The majority of penton base molecules avoided the lysosome while facilitating early vesicle release of low molecular weight dextran molecules. In further support of a vesicle escape capacity, a subpopulation of internalized penton base appeared to enter the nucleus, as observed by high-resolution confocal microscopy and cell fractionation. As a confirmation of these findings, we demonstrate that a recombinant penton base facilitated cytosolic entry of an siRNA molecule as observed by RNA interference of a marker gene. Based on our findings here, we suggest that whereas soluble penton base proteins may enter cells through clathrinand non-clathrin-mediated pathways, vesicle escape and nuclear delivery appear to be supported by a clathrin-mediated pathway. As our previous efforts have focused on utilizing recombinant penton base proteins as delivery agents for therapeutics, these findings allow us to evaluate the use of the penton base as a cell entry and intracellular trafficking agent, and may be of interest concerning the development of vectors for efficient delivery of therapeutics to cells. Gene Therapy (2006) 13, 821-836.

show abstract

“…We recently reported that SV40 entry is promoted by an intracellular signal that SV40 induces from the cell surface (Dangoria et al, 1996). More recently, we found that signal transmission by SV40 requires the functional integrity of the low-density membrane microdomain that surrounds the caveolae (Y. Chen & L. C. Norkin, unpublished results).…”

Section: Discussionmentioning

confidence: 99%

MHC class I molecules are enriched in caveolae but do not enter with simian virus 40.

Anderson

Chen

Norkin

1998

Journal of General Virology

View full text Add to dashboard Cite

Simian virus 40 (SV40) binds to MHC class I molecules anywhere on the cell surface and then enters through caveolae. The fate of class I molecules after SV40 binding is not known. Sensitivity of 125 I-surface-labelled class I molecules to papain cleavage was used to distinguish internalized class I molecules from class I molecules remaining at the cell surface. Whereas the caveolae-enriched membrane microdomain was found to also be enriched for class I molecules, no internalized papain-resistant 125 I-surface-labelled class I molecules could be detected at any time in either control cells or in

show abstract

Extracellular Simian Virus 40 Induces an ERK/MAP Kinase-independent Signalling Pathway that Activates Primary Response Genes and Promotes Virus Entry

Abstract: Simian virus

Cited by 71 publications

References 56 publications

Intracellular trafficking of nonviral vectors

Intracellular trafficking of nonviral vectors

Typical and atypical trafficking pathways of Ad5 penton base recombinant protein: implications for gene transfer

MHC class I molecules are enriched in caveolae but do not enter with simian virus 40.

Contact Info

Product

Resources

About