Extracellular Silica Nanocoat Confers Thermotolerance on Individual Cells: A Case Study of Material‐Based Functionalization of Living Cells

Wang, Guangchuan; Wang, Lijun; Liu, Peng; Yang, Yan; Xu, Xurong; Tang, Ruikang

doi:10.1002/cbic.201000494

Cited by 73 publications

(67 citation statements)

References 79 publications

(43 reference statements)

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“…As shown in Figure 2C and E, MSNs and A-MSNs could readily aggregate on the surfaces of the plant cells [28]. Compared with the interior of the plant cells alone (shown in Figure 2B), the concentration of silicon significantly increased from 0.01% to 1.20% (or 6.85%) in the plant cells after the uptake of MSNs or A-MSNs ( Figure 2D and F), which directly confirmed the passage of MSNs into the walled plant cells.…”

Section: Uptake Of Msns By Liriodendron Hybrids Suspension Cellsmentioning

confidence: 95%

Highly efficient uptake of ultrafine mesoporous silica nanoparticles with excellent biocompatibility by Liriodendron hybrid suspension cells

Xia

Dong

Zhang

et al. 2012

Sci. China Life Sci.

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The characteristics of the interactions co-cultures of ultrafine mesoporous silica nanoparticles (MSNs) and the Liriodendron hybrid suspension cells were systematically investigated using laser scanning confocal microscope (LSCM) and scanning electron microscopy (SEM). Using fluorescein isothiocyanate (FITC) labeling, the LSCM observations demonstrated that MSNs (size, 5-15 nm) with attached FITC molecules efficiently penetrated walled plant cells through endocytic pathways, but free FITC could not enter the intact plant cells. The SEM measurements indicated that MSNs readily aggregated on the surface of intact plant cells, and also directly confirmed that MSNs could enter intact plant cells; this was achieved by determining the amount of silicon present. After 24 h of incubation with 1.0 mg mL 1 of MSNs, the viability of the plant cells was analyzed using fluorescein diacetate staining; the results showed that these cells retained high viability, and no cell death was observed. Interestingly, after the incubation with MSNs, the Liriodendron hybrid suspension cells retained the capability for plant regeneration via somatic embryogenesis. Our results indicate that ultrafine MSNs hold considerable potential as nano-carriers of extracellular molecules, and can be used to investigate in vitro gene-delivery in plant cells. Along with developments in the application of nanotechnology from animal science and medical research to plant science research, the impact of engineered nanomaterials on plant systems has attracted increasing attention, the areas including (i) the delivery of fertilizers, herbicides, pesticides and exogenous genes, (ii) the improvement of the growth of plants, and (iii) nanotoxicity research for plant cells [1][2][3][4][5][6][7][8][9][10]. However, compared with mammalian cells, the plant cell wall which is composed of cross-linked polysaccharides (cellulose, hemicelluloses, and pectin) represents an extra barrier surrounding the cell membrane that hinders the passage of nanoparticles into plant cells. To avoid the inhibiting effects of the plant cell wall, free protoplasts (which are prepared via the removal of the cell wall using cellulase treatments) have been used previously to study the internalization of various nanoparticles (e.g., CdSe/ZnS quantum dots (QDs), polystyrene nanospheres, poly(phenylene ethynelene) nanoparticles, and carbon nanomaterials) [11][12][13]. However, protoplast-based transformation methods hold disadvantages in that the viability of the protoplasts and their ability to divide are strongly reduced by the chemicals that are applied to disorganize the cell wall. For this reason, recent studies have focused on intact plant cells; it was found to that they are able to achieve endocytosis to directly internalize single-walled carbon nanotubes (CNTs), CdSe/ZnS QDs, or poly (amidoamine) dendrimer from the

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Section: Uptake Of Msns By Liriodendron Hybrids Suspension Cellsmentioning

confidence: 95%

Highly efficient uptake of ultrafine mesoporous silica nanoparticles with excellent biocompatibility by Liriodendron hybrid suspension cells

Xia

Dong

Zhang

et al. 2012

Sci. China Life Sci.

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“…Several examples have recently been reported, including introducing superparamagnetic nanoparticles for magnetic eld responses, 24,25 silica to enhance thermal durability, 26 and graphene to impart electrical conductivity. 27,28 Another potential encapsulating material for microorganisms is graphene oxide (GO) which has a number of advantageous properties such as biodegradability, 29 exibility, 30 robustness, 30 transparency 30 and amphiphilicity.…”

Section: 23mentioning

confidence: 99%

Microencapsulation of bacterial strains in graphene oxide nano-sheets using vortex fluidics

et al. 2015

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Wrapping bacterial cells with graphene oxide sheets using a vortex fluidic device (VFD) effectively limits cellular growth for a certain time period whilst sustaining biological activity. This simple and benign method in preparing such a composite material relies on the shear within the film in the device without compromising the cellular viability. In principle, the process is scalable for large volumes, for operating the VFD(s) under continuous flow mode. Moreover, acquiring SEM images was possible without precoating the composite material with a metallic film, with limited charging effects. This establishes the potential for interfacing material with graphene oxide, which could be extended to more conductive graphene layers, as an effective approach for simplifying characterization using SEM.

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“…A natural virus cannot induce biomineralization by itself during its normal life cycle. In contrast, biomineralization in organisms is generally controlled using biomacromolecules in a mild biological system, and some polypeptides and macromolecules have been used to induce biomineralization (20)(21)(22)(23)(24). For example, several peptides have been genetically incorporated into the M13 bacteriophage and plant viruses as nucleators to direct nanomaterial synthesis (25,26) or as specific nucleators for CaP mineralization in living systems (23,(27)(28)(29).…”

mentioning

confidence: 99%

Rational design of thermostable vaccines by engineered peptide-induced virus self-biomineralization under physiological conditions

Wang

Cao

Chen

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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142

130

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The development of vaccines against infectious diseases represents one of the most important contributions to medical science. However, vaccine-preventable diseases still cause millions of deaths each year due to the thermal instability and poor efficacy of vaccines. Using the human enterovirus type 71 vaccine strain as a model, we suggest a combined, rational design approach to improve the thermostability and immunogenicity of live vaccines by self-biomineralization. The biomimetic nucleating peptides are rationally integrated onto the capsid of enterovirus type 71 by reverse genetics so that calcium phosphate mineralization can be biologically induced onto vaccine surfaces under physiological conditions, generating a mineral exterior. This engineered self-biomineralized virus was characterized in detail for its unique structural, virological, and chemical properties. Analogous to many exteriors, the mineral coating confers some new properties on enclosed vaccines. The self-biomineralized vaccine can be stored at 26°C for more than 9 d and at 37°C for approximately 1 wk. Both in vitro and in vivo experiments demonstrate that this engineered vaccine can be used efficiently after heat treatment or ambient temperature storage, which reduces the dependence on a cold chain. Such a combination of genetic technology and biomineralization provides an economic solution for current vaccination programs, especially in developing countries that lack expensive refrigeration infrastructures.genetic engineering | vaccine design | shell

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Extracellular Silica Nanocoat Confers Thermotolerance on Individual Cells: A Case Study of Material‐Based Functionalization of Living Cells

Cited by 73 publications

References 79 publications

Highly efficient uptake of ultrafine mesoporous silica nanoparticles with excellent biocompatibility by Liriodendron hybrid suspension cells

Highly efficient uptake of ultrafine mesoporous silica nanoparticles with excellent biocompatibility by Liriodendron hybrid suspension cells

Microencapsulation of bacterial strains in graphene oxide nano-sheets using vortex fluidics

Rational design of thermostable vaccines by engineered peptide-induced virus self-biomineralization under physiological conditions

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