Extracellular secretion of pectate lyase by the Erwinia chrysanthemi out pathway is dependent upon Sec-mediated export across the inner membrane

Stable periplasmic secretion intermediate in the general secretory pathway of Escherichia coli.

“…For example, secretion of proaerolysin by A.hydrophila (Jiang and Howard, 1992) is almost complete within 4-10 min depending on the experimental conditions (Howard and Buckley, 1985), while PelE pectate lyase of E. chrysanthemi is secreted within 1 min of E. coli carrying the cloned pelE and its cognate secretion genes (out;He et al, 1991a,b). Indeed, PelE secretion occurs so quickly that it is unlikely to transit via a free periplasmic intermediate similar to that demonstrated here with overproduced MalE-PulA, although periplasmic accumulation of PelE can be imposed by high level production (He et al, 1991b) or by mutations in the out genes (Andro et al, 1984). Similarly, when the P.aeruginosa PAK toxin A gene is expressed in the hypotoxigenic P.aeruginosa mutant PAO-T1, 80% of the toxin is secreted but the remaining 20% could not be detected in the periplasm (Hamood et al, 1989 Several hypotheses can be envisioned for the comparatively slow secretion of overproduced MalE -PulA.…”

Protein secretion and the pathogenesis of bacterial infections

“…Studies on many different type II substrates have identified a large variety of domains and amino acids that are required for secretion (Hamood et al 1989;Kornacker and Pugsley 1990;He et al 1991b;Wong and Buckley 1991;Py et al 1993;Palomaki and Saarilahti 1995;Sauvonnet et al 1995;Lu and Lory 1996). For example, two spatially separated domains of pullulanase (PulA) seem required for substrate recognition (Sauvonnet and Pugsley 1996).…”

Interaction domains in the Pseudomonas aeruginosa type II secretory apparatus component XcpS (GspF)

“…First, signal sequence-bearing exoproteins are translocated across the cytoplasmic membrane via the Sec or the Tat machinery (He et al, 1991;Voulhoux et al, 2001). After release in the periplasm, unfolded exoproteins adopt their tertiary conformation.…”