Extracellular Recordings From Patterned Neuronal Networks Using Planar Microelectrode Arrays

Abstract

Neuronal cell networks have been reconstructed on planar microelectrode arrays (MEAs) from dissociated hippocampal pyramidal neurons. Microcontact printing (microCP) and a photoresist-liftoff method were used to selectively localize poly-L-lysine (PLL) on the surface of MEAs. Haptotaxis led to the organization of the neurons into networks localized adjacent to microelectrodes. Various grids of PLL with 2-25-microm-wide lines spaced by 50-200 microm with 15-25-microm nodes at intersection points were used to guide cell body attachment and neurite outgrowth. Bursting activity with spike amplitude attenuation was observed, and multichannel recordings detected instances of coincident firing activity. Finally, we present here an extracellular recording from a approximately 2 microm bundle of guided neurites.

Action potential recording from dielectrophoretically positioned neurons inside micro-wells of a planar microelectrode array

“…Platinum black deposition (Novak and Wheeler, 1988;Bove et al, 1995;Oka et al, 1999;James et al, 2004) is being considered for lowering the electrode impedance, and silicon nitride (Kovacs et al, 1994;Nisch et al, 1994;Nam et al, 2004) for insulating the tracks and the electrode site areas beneath the entrance of microtrenches. These features were absent from our pMEAs due to lack of necessary 23 resources.…”

“…Techniques involving the implementation of three-dimensional microstructures over the pMEA electrodes (Jimbo et al, 1993;Maher et al, 1999;Griscom et al, 2002;Suzuki et al, 2004;Morin et al, 2006), and chemically patterned growth substrates (Wyart et al, 2002;Nam et al, 2004;James et al, 2004) were used successfully. In the case of growth substrates, the pMEA is micro-stamped with proteins that promote neural adhesion to the substrate in order to organise them in a predetermined manner; however, it has been reported that cell survival is decreased due to the chemical modification of the substrate (Branch et al, 2000).…”

Low-density neuronal networks cultured using patterned poly-l-lysine on microelectrode arrays

“…Planar microelectrode arrays (MEAs) provide a means for long-term, simultaneous recording of electrical activities from neural networks (Jimbo et al, 1992;James at al., 2004;Chang et al, 2001;Maher et al, 1999). Previous studies of neuronal networks on MEAs have used a large numbers of cells that are generally randomly organized on culture surfaces.…”

Applying Information Theory to Neuronal Networks: From Theory to Experiments

“…MEAs are non-cell-invasive and can be used in vitro [36] as well as in vivo (with so called polytrodes) to make multi-channel recordings with a very high temporal resolution [37]. In vitro MEAs can contain up to 10,000 electrodes and in vivo the polytrode is limited to about 100 electrodes.…”