Extracellular proteinase inhibitor-accelerated apoptosis is associated with B cell activating factor in mammary epithelial cells

The Novel Activated Microglia/Macrophage WAP Domain Protein, AMWAP, Acts as a Counter-Regulator of Proinflammatory Response

“…Our ChIP-Chip and ChIP-PCR assays have revealed that the AMWAP promoter, but not the Expi region are bound by PU.1 and the coactivators Cbp and p300. These findings are consistent with microglia/macrophagerestricted expression of AMWAP and the ubiquitous expression of Expi (58). Likely, the different expression patterns and regulation of both genes coincides with different cellular functions.…”

“…Likely, the different expression patterns and regulation of both genes coincides with different cellular functions. In line with this, Expi was identified as a protein overexpressed in nonmetastatic mammary cancer cells (59,60) and ectopic expression of Expi resulted in induced apoptosis of mammary epithelial cells (58).…”

Induction of Serum Amyloid A Genes Is Associated with Growth and Apoptosis of HC11 Mammary Epithelial Cells

“…[4][5][6] HC11 cells were grown to confluence in growth medium (GM) containing 10% FBS and EGF for 2 d, and then cultured in differentiation medium (DM) and/or serum-free medium (SFM). In HC11 cells undergoing differentiation, expression of SAA1, SAA2, and SAA3 mRNAs was induced, and the levels of induction were maintained when the cells were switched to SFM (Fig.…”

“…The HC11 cell line, a clonal mouse mammary epithelial cell line derived from spontaneously immortalized COMMA1D epithelial cells, [4][5][6] was cultured in growth medium at 37 C in 5% CO 2 . The growth medium (GM) consisted of RPMI 1640 (Gibco BRL, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS; Gibco BRL), 1% glutamine (Gibco BRL), 50 mg/ml gentamycin (Gibco BRL), 5 mg/ml insulin (Sigma, St. Louis, MO), and 10 mg/ml EGF (Sigma).…”

“…2,3) Previously, we identified several involution-induced genes, including SAA3, WDNM1, and sulfated glycoprotein-2, in bovine and mouse mammary glands. [4][5][6][7] SAA has been classified into two sub-families: acutephase SAAs (SAA1 and SAA2), which are dramatically up-regulated as acute-phase reactants during inflammation, 8) and constitutive SAA, which is constitutively present in serum and is not significantly increased during inflammation. 9) SAA1 and SAA2 are 95% homologous in both their coding and noncoding regions, and are coordinately induced in response to inflammation.…”