Extracellular protease in Actinomycetes culture supernatants inhibits and detaches Staphylococcus aureus biofilm formation

Abstract

Bacterial biofilms are associated with chronic infections due to their resistance to antimicrobial agents. Staphylococcus aureus is a versatile human pathogen and can form biofilms on human tissues and diverse medical devices. To identify novel biofilm inhibitors of S. aureus, the supernatants from a library of 458 Actinomycetes strains were screened. The culture supernatants (1% v/v) of more than 10 Actinomycetes strains inhibited S. aureus biofilm formation by more than 80% without affecting the growth. The culture supernatants of these biofilm-reducing Actinomycetes strains contained a protease (equivalent to 0.1 μg proteinase K ml(-1)), which both inhibited S. aureus biofilm formation and detached pre-existing S. aureus biofilms. This study suggests that protease treatment could be a feasible tool to reduce and eradicate S. aureus biofilms.

Bdellovibrio bacteriovorus Inhibits Staphylococcus aureus Biofilm Formation and Invasion into Human Epithelial Cells

“…S. aureus biofilm formation, however, is susceptible to proteinase K28. With HI B. bacteriovorus HD100 cultures releasing proteases into the media, we were intrigued by the idea that this predator and its secreted hydrolytic enzymes may block biofilm formation by S. aureus .…”

Role of Extracellular Proteases in Biofilm Disruption of Gram Positive Bacteria with Special Emphasis on Staphylococcus aureus Biofilms

“…The culture supernatants of these biofilm reducing actinomycetes were found to contain a protease which in addition to inhibiting biofilm formation itself also disrupted preestablished biofilm structures. This study by Park et al [31] suggests that protease treatment could be a feasible tool to reduce or eradicate biofilm mediated persistent S. aureus infections [31].…”

“…Park et al [31] identified some novel biofilm inhibitors of S. aureus, by screening culture supernatant of 458 actinomycete isolates. The culture supernatants in the ratio of 1% (v/v) of more than 10 Actinomycete isolates inhibited S. aureus biofilm formation by more than 80% without inhibiting growth of the test culture.…”

“…BFI 250 and the strain Kribbella sp. BFI 1562 [31]. The culture supernatants of these biofilm reducing actinomycetes were found to contain a protease which in addition to inhibiting biofilm formation itself also disrupted preestablished biofilm structures.…”

Antimicrobial activity and FTIR characterization of lapachol derivatives against Staphylococcus aureus

“…This is due, for example, to the production of toxins (Wu et al, 2016). However, the formation of biofilm, a structure formed by a mucopolysaccharide matrix, is still considered one of the main forms of pathogenesis (Paharik e Horswill, 2016;Park et al, 2012).…”